The succinic dehydrogenase and cytochrome c oxidase activities of guinea-pig skin after mild heat damage

Carney, Shirley A.; Hall, Mark; Ricketts, C. R.

doi:10.1111/j.1365-2133.1976.tb04386.x

Cited by 8 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data presented in this paper demonstrate that the respiration of heat-damaged skin can be protected if a substrate is supplied which is directly oxidizable by the respiratory chain. These findings also narrow the area in which the origin of heat-produced failure of oxygen uptake must be sought, since it has previously been shown that glycolysis is also more resistant to heat inhibition than is oxygen uptake (Carney et al, 1976a). The earliest heat-induced metabolic failure must therefore occur within the Krebs cycle (Fig.…”

Section: Discussionmentioning

confidence: 72%

“…In skin the enzymes of the respiratory chain are relatively resistant to heat damage. Previous work by Carney et al (1976b) demonstrated that pre-treatment temperatures of 45 3 C and 45 9 C were required to produce 50",, inhibition of succinic dehydrogenase and cytochrome C oxidase activities respectively. The data presented in this paper demonstrate that the respiration of heat-damaged skin can be protected if a substrate is supplied which is directly oxidizable by the respiratory chain.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of substrate on the uptake of oxygen and sulphate ions by heat-damaged skin

Carney

Lawarence

1979

Br J Dermatol

Self Cite

View full text Add to dashboard Cite

Respiration and sulphate uptake have been measured in guinea-pig skin subjected to heat damage and maintained on a medium containing sodium succinate. Succinate was found to have a protective effect against loss of oxygen uptake, a higher temperature being required for 50% effect than that reported with glucose as substrate. The protective effect did not extend to sulphate incorporation, suggesting that this process is itself heat-labile and that its failure with heat damage is not directly caused by diminution of the energy supply.

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

The effect of substrate on the uptake of oxygen and sulphate ions by heat-damaged skin

Carney

Lawarence

1979

Br J Dermatol

Self Cite

View full text Add to dashboard Cite

show abstract

Hyperthermic potentiation.Biological aspects and applications to radiation therapy

Leith

Miller

Gerner

et al. 1977

Cancer

110

View full text Add to dashboard Cite

Experimental studies have provided evidence that hyperthermia may be an effective agent, either alone or in combination with ionizing radiation, in the treatment of cancer. Results have shown that temperatures in the range of 42 degrees to 45 degrees C: 1) are cytotoxic, with cell lethality showing little or no dependence on levels of oxygenation; 2) inhibit the recovery of cells from sub-lethal and potentially lethal radiation damage while enhancing the levels of lethal damage; and 3) may be combined with x-irradiation in a manner to improve therapeutic ratios. The observed interaction between hyperthermia and x-rays may in part be due to differences in the Age Response Functions and reassortment of cycling cells to these two agents. Hyperthermia may also greatly change repopulation and re-oxygenation parameters in irradiated tumor and normal tissue volumes. An overall consideration of these and other factors is essential in the design of optimal schedules of combined hyperthermia and x-irradiation treatments in the management of malignant disease.

show abstract

Histochemical model studies of enzyme activity after thermal damage

Prosperi¹,

Raap

Ploeg

1982

Histochemistry

View full text Add to dashboard Cite

The reliability of histochemical determinations of the enzyme activity after thermal damage has been studied with the aid of two model systems. Polyacrylamide films and erythrocyte ghosts containing either beta-glucuronidase or alkaline phosphatase, were submitted to heating and the activities retained were assessed both biochemically and histochemically. For the enzymes studied, the results show that tissue alterations induced by heat can influence histochemical reaction procedures, and that with these model systems, factors which are important for the histochemical quantitation of enzyme activities in thermally damaged tissues can be evaluated quantitatively. Potentialities of these model systems in the study of evaluating thermal damage through histochemical enzyme activity determinations, are discussed.

show abstract

The succinic dehydrogenase and cytochrome c oxidase activities of guinea-pig skin after mild heat damage

Cited by 8 publications

References 12 publications

The effect of substrate on the uptake of oxygen and sulphate ions by heat-damaged skin

The effect of substrate on the uptake of oxygen and sulphate ions by heat-damaged skin

Hyperthermic potentiation.Biological aspects and applications to radiation therapy

Histochemical model studies of enzyme activity after thermal damage

Contact Info

Product

Resources

About