The sulfated O-specific polysaccharide from the marine bacterium Cobetia pacifica KMM 3879T

Lipid A Structure and Immunoinhibitory Effect of the Marine Bacterium Cobetia pacifica KMM 3879T

“…The LPS was extracted from the dried bacterial pellet through the hot phenol/water procedure . The nature and relative purity of the extracted material was established by SDS‐PAGE after silver‐nitrate‐gel staining, which showed a ladder‐like profile in the upper part of the gel, typical of an S‐LPS (Figure S1) . To remove cell contaminants such as proteins and nucleic acids, the extracted LPS was subjected to an extensive enzymatic digestion, followed by dialysis and ultracentrifugation.…”

“…The work described in this paper is focussed on the LPS from Cobetia pacifica KMM 3879 T , a marine bacterium isolated from a sandy sediment (depth 1 m) sample collected from the shore of the Sea of Japan (Russia). We have previously established that this bacterium expresses an S‐LPS with an uncommon O‐chain structure made up of a trisaccharide repeating unit containing rhamnose, d ‐glucose 3‐sulfate, and d ‐galactose 3‐sulfate . In this paper we describe the structural characterization of the lipid A moiety, which turned out to be a complex mixture of variously acylated species.…”

Gram‐Negative Extremophile Lipopolysaccharides: Promising Source of Inspiration for a New Generation of Endotoxin Antagonists

“…The authors hypothesized that the presence of the sulfate groups might provide protection to the bacteria by facilitating their adaptation toward the highly variable marine environment conditions. This hypothesis was supported by the discovery of a further sulfated O‐chain structure making up the LPS from another marine bacterium, Cobetia pacifica , isolated from a sandy sediment sample collected at a depth of 1 m from the shore of the Sea of Japan, Russia , …”

Structure of the O-specific polysaccharide from the deep-sea marine bacterium Idiomarina abyssalis КММ 227T containing a 2-O-sulfate-3-N-(4-hydroxybutanoyl)-3,6-dideoxy-d-glucose

“…abyssalis KMM 227 T has the following structure: [18][19][20] Interesting to note that 2-acetamido-2-deoxy-hexuronic acids with galacto-and mannoconfigurations were found in the OPS from other deep-sea marine gram-negative bacteria. 21,22 Comparative study of cell surface structures of some marine bacteria will help to define the …”