The support of bone marrow stromal cell differentiation by airbrushed nanofiber scaffolds

Airbrushed Composite Polymer Zr-ACP Nanofiber Scaffolds with Improved Cell Penetration for Bone Tissue Regeneration

“…32 To assess the biorelevance of this new network macromorphology, hBMSCs were seeded on airbrushed and electrospun PCL scaffolds. The depths of cell penetration were greater in the airbrushed polymer PCL networks than in the electrospun scaffolds.…”

“…Our observations and results reported in literature indicated that the type of polymer used had an effect on the final polymer fiber diameter. 32,35 The largest fiber diameter was measured for PMMA polymer (d ave = 0.79 -0.2 mm), followed by P-DL-LA (d ave = 0.28 -0.05 mm) and PCL (d ave = 0.21 -0.07 mm). All three polymers were able to incorporate up to 20% (w/w) dry Zr-ACP powder ( Fig.…”

“…In our previous publication, we reported that the measured relative pore size and porosity were higher in airbrushed scaffolds. 32 The cells were fixed, as described above, and fluorescently stained to image the cell nucleus and determine cell penetration rates (Hoechst 33342) at day 1. Four PCL specimens of both airbrushed and electrospun scaffolds were scanned through confocal microscopy (TCS SP5 broadband, 10 · /0.3 NA objective; Leica).…”

“…13,31 As shown in this and our earlier studies, nanofiber airbrushing provides a solution to many of these limitations. 32 Certain bulk material properties and scaffold morphology, including the Young's modulus, network pore size and porosity were reported to be different between electrospun and airbrushed scaffolds and potentially advantageous for tissue regeneration applications. 32 Therefore, the objective of this study was to (a) evaluate the use of an airbrush device as a method of synthesizing Zr-ACP composite nanofiber scaffolds from various polymers; (b) evaluate the extent of cell penetration within more open structure of the nanofiber networks; (c) investigate the ability of a composite fiber to release Zr-ACP biomolecules; and (d) examine the effect of Zr-ACP on hBMSC growth and differentiation.…”

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

“…[1][2][3][4][5][6] Biomimetic, nonwoven, nanofibrous scaffolds composed of large networks of interconnected fibers and pores may be fabricated via electrospinning. 7 Electrospun nanofibrous scaffolds of synthetic and natural polymers, such as polycaprolactone (PCL) and gelatin, 8 have been fabricated to regenerate organs or tissues, including bone, 9 cartilage, 10 skin, 11 neurons, 12 and blood vessels. 13 A major limitation of traditional electrospun fibers is their small pore size, which restricts cell growth to the surface of the scaffold without any cell infiltration.…”