The Suppressive Effects of Oxcarbazepine on Mechanical and Cold Allodynia in a Rat Model of Neuropathic Pain

Jang, Yeon; Kim, Eun S.; Park, Soo S.; Lee, Jaemin; Moon, Dong Eon

doi:10.1213/01.ane.0000167283.80463.d7

Cited by 23 publications

(14 citation statements)

References 22 publications

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“…Therefore, developing novel therapeutic strategies is necessary. Peripheral nerve injury such as CCI leads to mechanical and thermal hyperalgesia [42–45] and also provokes a reaction in peripheral immune and spinal cord glial cells [46]. The current study showed that AS reduced CCI-induced neuropathic pain.…”

Section: Discussionmentioning

confidence: 50%

The nitroxyl donor, Angeli’s salt, reduces chronic constriction injury-induced neuropathic pain

Longhi-Balbinot

Rossaneis

Pinho‐Ribeiro

et al. 2016

Chemico-Biological Interactions

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Chronic pain is a major health problem worldwide. We have recently demonstrated the analgesic effect of the nitroxyl donor, Angeli’s salt (AS) in models of inflammatory pain. In the present study, the acute and chronic analgesic effects of AS was investigated in chronic constriction injury of the sciatic nerve (CCI)-induced neuropathic pain in mice. Acute (7th day after CCI) AS treatment (1 and 3 mg/kg; s.c.) reduced CCI-induced mechanical, but not thermal hyperalgesia. The acute analgesic effect of AS was prevented by treatment with 1H-[1,2, 4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ, a soluble guanylate cyclase inhibitor), KT5823 (an inhibitor of protein kinase G [PKG]) or glibenclamide (GLB, an ATP-sensitive potassium channel blocker). Chronic (7–14 days after CCI) treatment with AS (3 mg/kg, s.c.) promoted a sustained reduction of CCI-induced mechanical and thermal hyperalgesia. Acute AS treatment reduced CCI-induced spinal cord allograft inflammatory factor 1 (known as Iba-1), interleukin-1β (IL-1β), and ST2 receptor mRNA expression. Chronic AS treatment reduced CCI-induced spinal cord glial fibrillary acidic protein (GFAP), Iba-1, IL-1β, tumor necrosis factor-α (TNF-α), interleukin-33 (IL-33) and ST2 mRNA expression. Chronic treatment with AS (3 mg/kg, s.c.) did not alter aspartate aminotransferase, alanine aminotransferase, urea or creatinine plasma levels. Together, these results suggest that the acute analgesic effect of AS depends on activating the cGMP/PKG/ATP-sensitive potassium channel signaling pathway. Moreover, chronic AS diminishes CCI-induced mechanical and thermal hyperalgesia by reducing the activation of spinal cord microglia and astrocytes, decreasing TNF-α, IL-1β and IL-33 cytokines expression. This spinal cord immune modulation was more prominent in the chronic treatment with AS. Thus, nitroxyl limits CCI-induced neuropathic pain by reducing spinal cord glial cells activation.

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Section: Discussionmentioning

confidence: 50%

The nitroxyl donor, Angeli’s salt, reduces chronic constriction injury-induced neuropathic pain

Longhi-Balbinot

Rossaneis

Pinho‐Ribeiro

et al. 2016

Chemico-Biological Interactions

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“…After surgery, the rats were allowed to recover for 7 days before starting the behavioral tests. Those animals that showed a foot withdrawal response to the von Frey filaments (Semmes Weinstein Von Frey Aesthesiometers, Stoelting CO, IL, USA) with an applied bending force of 4 g or less were considered to be neuropathic [20–22], and they were used in the tests. The rats that exhibited motor deficiency (such as paw dragging or limping) or those that failed to exhibit subsequent mechanical allodynia were excluded.…”

Section: Methodsmentioning

confidence: 99%

The antinociceptive effect of acetaminophen in a rat model of neuropathic pain

Im¹,

Jung²,

Kim³

et al. 2012

The Kaohsiung J of Med Scie

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Acetaminophen is one of the most popular and widely used analgesics for the treatment of pain and fever but few studies have evaluated its effects on neuropathic pain. This study examined the effect of acetaminophen on thermal hyperalgesia, mechanical and cold allodynia in a rat model of neuropathic pain. Male Sprague-Dawley rats were prepared by tightly ligating the left L5 and L6 spinal nerves to produce a model of neuropathic pain. Sixty neuropathic rats were assigned randomly into six groups. Normal saline and acetaminophen (25, 50, 100, 200 and 300 mg/kg) were administered intraperitoneally to these individual groups. Thermal hyperalgesia, mechanical and cold allodynia were examined at preadministration and at 15, 30, 60, 90, 120, 180, 240 and 360 min after administering the drug. Mechanical allodynia was quantified by measuring the paw withdrawal threshold to stimuli with von Frey filaments. Cold allodynia was quantified by measuring the frequency of foot lift after applying 100% acetone. Thermal hyperalgesia was quantified by measuring the thermal withdrawal threshold. The rotarod performance was measured to detect any drug-induced adverse effects, such as drowsiness. The hepatic and renal adverse effect was also assessed by measuring the serum levels of aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine. The paw withdrawal thresholds to mechanical stimuli and the thermal withdrawal threshold were increased significantly and withdrawal frequencies to cold stimuli were reduced by acetaminophen administration in a dose-dependent manner. Acetaminophen reduces thermal hyperalgesia, mechanical and cold allodynia in a rat model of neuropathic pain, and might be useful for managing neuropathic pain.

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“…The animals we used in the tests were those that showed a foot withdrawal response to von Frey filaments (Semmes Weinstein von Frey esthesiometer, Stoelting Co., IL, USA), with an applied bending force of B 4 g considered neuropathic. 19 The rats that exhibited motor deficiency, such as paw dragging or limping, or those that failed to exhibit subsequent mechanical allodynia were excluded from any further testing.…”

Section: Spinal Nerve Ligationmentioning

confidence: 99%

Attenuation of neuropathy-induced allodynia following intraplantar injection of pregabalin

Park

Joo

Chang

et al. 2010

Can J Anesth/J Can Anesth

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Purpose Pregabalin exhibits potent anticonvulsant, analgesic, and anxiolytic activity in animal models. However, few studies have evaluated pregabalin's potential peripheral effects on neuropathic pain. The aim of this study was to evaluate the peripheral analgesic effects of pregabalin in a rat model of neuropathic pain. Methods Male Sprague-Dawley rats were prepared by ligating the left L5 and L6 spinal nerves to produce neuropathic pain. Sixty rats with neuropathic pain were randomly assigned to six groups. Normal saline (control) and pregabalin (10,20,30, and 50 mgÁkg -1 ) were administered to the plantar surface of the affected left hind paw. Pregabalin (50 mgÁkg -1 ) was administered into the unaffected contralateral paw in order to determine its systemic effect. Responses to mechanical, cold, and heat stimulation were recorded at 15,30, 60, 90, 120, 150, and 180 min after drug administration. Rotarod performance was measured to detect drug-induced side effects, including sedation and reduced motor coordination. Results Saline injected into the affected paw and a pregabalin dose of 50 mgÁkg -1 injected into the contralateral paw showed no differences for mechanical, cold, and heat allodynia. Administration of pregabalin to the affected left hind paw in the dose range of 10-50 mgÁkg -1 resulted in a dose-dependent increase in thresholds to mechanical, cold, and heat stimulation. Conclusion Peripherally administered pregabalin attenuates mechanical, cold, and heat allodynia in a rat model of neuropathic pain. RésuméObjectif La pre´gabaline de´montre une puissante activiteá nticonvulsivante, analge´sique et anxiolytique dans les mode`les animaux. Ne´anmoins, il n'existe que peu d'e´tudes ayant e´value´les effets pe´riphe´riques potentiels de la pre´gabaline sur la douleur neuropathique. L'objectif de cette e´tude e´tait d'examiner les effets analge´siques pe´riphe´riques de la pre´gabaline dans un mode`le de douleur neuropathique chez le rat. Méthode Des rats Sprague-Dawley maˆles ont e´teṕ re´pare´s en ligaturant les nerfs rachidiens L5 et L6 afin de cre´er une douleur neuropathique. Soixante rats souffrant de douleur neuropathique ont e´te´ale´atoirement re´partis en six groupes. Du se´rum physiologique (te´moin) et de la pre´gabaline (10, 20, 30, et 50 mgÁkg -1 ) ont e´te´administre´s a`la surface plantaire de la patte arrie`re gauche affecte´e. De la pre´gabaline (50 mgÁkg -1 ) a e´te´administre´e à la patte controlate´rale non affecte´e afin de de´terminer son effet syste´mique. Les re´actions aux stimulations me´caniques, au froid et a`la chaleur ont e´te´enregistre´es a1 5, 30, 60, 90, 120, 150 et 180 min apre`s l'administration du me´dicament. La performance au test de la tige tournante a e´te´mesure´e afin de de´pister les effets secondaires provoque´s par le me´dicament, notamment la se´dation et une re´duction de la coordination motrice.This article is accompanied by an editorial. Please see Can J Anesth 2010; 57(7).

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The Suppressive Effects of Oxcarbazepine on Mechanical and Cold Allodynia in a Rat Model of Neuropathic Pain

Cited by 23 publications

References 22 publications

The nitroxyl donor, Angeli’s salt, reduces chronic constriction injury-induced neuropathic pain

The nitroxyl donor, Angeli’s salt, reduces chronic constriction injury-induced neuropathic pain

The antinociceptive effect of acetaminophen in a rat model of neuropathic pain

Attenuation of neuropathy-induced allodynia following intraplantar injection of pregabalin

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