The Surface Activity of Purified Ocular Mucin at the Air-Liquid Interface and Interactions With Meibomian Lipids

Millar, Thomas J.; Tragoulias, Sophia T; Anderton, Philip J.; Ball, Malcolm S.; Miano, Fausto; Dennis, Gary R.; Mudgil, Poonam

doi:10.1097/01.ico.0000164779.87795.3c

Cited by 60 publications

(68 citation statements)

References 31 publications

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“…As increases these thick lipid islands tend to grow and merge and to fulfil the field of view while the black stripes almost disappear. Similar images are already observed with fluorescence microscopy by Millar et al [20] in surface balance studies of MGS, and interpreted as lipid islands with some water channels in it, that gradually close during the film compression. The size of the aggregates and the intensity of the BAM images indicate that MGS actually does not exist as a monolayer even at zero surface pressure but instead forms thicker multilayer structures.…”

Section: Discussionsupporting

confidence: 85%

“…1. The isotherms of the three "pure" compounds, MGS, SM and DPPC coincide well with the previously published results obtained under similar conditions [18][19][20]29,34]. MGS isotherm shows low slope (i.e.…”

Section: Quasi-equilibrium Compression Of Mixed Films Of Mgs and Plssupporting

confidence: 90%

“…MGS isotherm shows low slope (i.e. high compressibility) with characteristic inflexion at 15-17 mN and maximal surface pressure of 25 mN/m as reported in [18][19][20].…”

Section: Quasi-equilibrium Compression Of Mixed Films Of Mgs and Plsmentioning

confidence: 57%

“…As it is easier to obtain sufficient amount of material of consistent composition, bovine MGS is the commonly used standard in studies of meibium surface properties [18,20,28]. MGS was extracted from bovine eyelids obtained from the abattoir by gently squeezing the lid borders as it was previously described [18,20,28]. The secretions were dissolved in chloroform, centrifuged and evaporated in a rotary concentrator at 25 • C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…For the purpose we formed mixed pseudo-binary films of MGS and polar lipid, Egg Sphingomyelin (SM) or Dipalmitoylphosphatidylcholine (DPPC), at the air/water interface of Langmuir surface balance. Langmuir surface balance was already successfully employed in pioneer studies of the interactions between tear proteins and meibium [18][19][20][21][22][23]. SM and DPPC are chosen because they were used in previous studies as synthetic mimics of TF polar lipids [24,25].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Georgiev

Kutsarova

Jordanova

et al. 2010

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tThe surface interactions of Meibomian gland secretion (MGS) with polar lipid (PL), Egg Sphingomyelin (SM) or Dipalmitoylphosphatidylcholine (DPPC), are studied in mixed pseudo-binary films formed at the air/water interface of Langmuir surface balance. The behavior of the mixed films during slow quasi-equilibrium compression and during fast dynamic compression-decompression is registered by measurements of surface pressure and surface potential, and by monitoring film morphology with Brewster Angle Microscopy (BAM). Quasi-equilibrium compression isotherms are used to calculate the excess Gibbs and Helmholtz energy of mixing between MGS and PLs and thus to evaluate the interactions between the lipid compounds at the interface. The effects of PLs on the mixed film's elastic moduli of area compressibility, morphology and capability to attain high surface pressures are also examined.PLs interact with MGS with different strength and in different manner: MGS-SM interaction is weak and might lead to interfacial disaggregation of the thick meibium domains when SM is in excess, while MGS-DPPC interaction is strong and results in the formation of thick lipid aggregates. Both PLs increase the mixed films reciprocal compressibility and capability to achieve higher surface pressures. The results demonstrate that in vitro studies of the surface interactions between MGS and PLs might be beneficial in the selection of PLs for artificial tear formulations and for examination on molecular scale of the possible role of PLs at the ocular surface.

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Section: Discussionsupporting

confidence: 85%

Section: Quasi-equilibrium Compression Of Mixed Films Of Mgs and Plssupporting

confidence: 90%

“…MGS isotherm shows low slope (i.e. high compressibility) with characteristic inflexion at 15-17 mN and maximal surface pressure of 25 mN/m as reported in [18][19][20].…”

Section: Quasi-equilibrium Compression Of Mixed Films Of Mgs and Plsmentioning

confidence: 57%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Georgiev

Kutsarova

Jordanova

et al. 2010

Colloids and Surfaces B: Biointerfaces

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Ocular Delivery of Biopharmaceuticals

Lorentz

Sheardown

2014

Mucosal Delivery of Biopharmaceuticals

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Tear Film Constituents and Medicines for Eyes Investigated as Langmuir Films

Torrent‐Burgués

2023

BioNanoSci.

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The tear film has an important role on the quality of the vision process and is of importance in the fields of ophthalmology and optometry. The lipid layer, the outermost part of the tear film, can be investigated with the Langmuir technique, as well as the interaction of components of the aqueous part of the tear film with those of the lipid layer. Dysfunctions in the stability and composition of the tear film are the causes of eye diseases, as the dry eye syndrome. For the treatment of this disease, the artificial tears are used, and one type of them are the lipid tears, which can be investigated with the Langmuir technique. This technique can also be used to investigate the influence on the lipid layer of other artificial tear components or medicines instilled in the eye.This review presents investigations where the Langmuir technique has been used in relation with the tear film. In particular, the surface properties of natural tears and of four commercial lipid-containing artificial tears have been reported and discussed in connection with their composition.

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The Surface Activity of Purified Ocular Mucin at the Air-Liquid Interface and Interactions With Meibomian Lipids

Cited by 60 publications

References 31 publications

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Ocular Delivery of Biopharmaceuticals

Tear Film Constituents and Medicines for Eyes Investigated as Langmuir Films

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