The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011

Prodělalová, Jana

doi:10.1016/j.meegid.2012.04.025

Cited by 58 publications

(67 citation statements)

References 22 publications

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“…The overall frequencies of each virus detected herein are similar to a study performed in the Czech Republic, in which EV-G was most commonly detected, followed by PTV and PSV in the 72 fecal samples evaluated from wild boars up to 1 year of age (Prodělalová 2012). Considering single and mixed infections, the results of both of these studies (Prodělalová 2012) are also convergent, with PTV and EV-G double infections detected in higher frequencies, followed by the triple viral infections.…”

Section: Discussionsupporting

confidence: 84%

“…Considering single and mixed infections, the results of both of these studies (Prodělalová 2012) are also convergent, with PTV and EV-G double infections detected in higher frequencies, followed by the triple viral infections. In both studies, PSV was detected with the least frequency.…”

Section: Discussionmentioning

confidence: 69%

“…The majorities of the PTV, PSV, and EV-G serotypes are non-pathogenic and circulate worldwide in asymptomatic domestic pigs (Cano-Gómez et al 2011, Prodělalová 2012). However, depending on the virus serotype and infection conditions, these picornaviruses may be considered important etiological agents of enteric, respiratory, reproductive, or neurological disorders (Bangari et al 2010, Ventura et al 2013.…”

Section: Introductionmentioning

confidence: 99%

“…Studies performed in different geographical regions throughout the world revealed that PTV, PSV, and EV-G are ubiquitous and have been reported in Asia (Kaku et al 2007, Lan et al 2011, Lin et al 2012, Moon et al 2012, Zhang et al 2012, Europe (Zell et al 2000, La Rosa et al 2006, Buitrago et al 2010, Sozzi et al 2010, Boros et al 2011, Prodělalová 2012, and the Americas (Bangari et al 2010, Salles et al 2011, Shan et al 2011, Deng et al 2012, Ventura et al 2013, Donin et al 2014.…”

Section: Introductionmentioning

confidence: 99%

“…Wild boars are susceptible to infections by viruses that also affect domestic pigs (Sus scrofa domesticus), such as porcine group A rotavirus (PoRVA), porcine circovirus type 2 (PCV2), hepatitis E virus (HEV), Aujeszky disease virus (ADV), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSF), torque teno sus virus (TTSuV), influenza A virus, and others (Ruiz-Fons et al 2008, Meng et al 2009, Okadera et al 2013, Biondo et al 2014. These animals are also susceptible to infections by PTV, PSV, and EV-G, which were described in asymptomatic wild boars in Hungary (Boros et al 2012a, Boros et al 2012b, Spain (Cano--Gómez et al 2013), and the Czech Republic (Prodělalová 2012).…”

Section: Introductionmentioning

confidence: 99%

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Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

et al. 2015

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RESUMO.-[Estudo molecular de teschovírus suíno, sapelovírus suíno e enterovírus G em javalis (Sus scrofa scrofa) de cativeiro no Paraná.] Teschovírus suíno (PTV), sapelovírus suíno (PSV) e enterovírus G (EV-G) são agentes infecciosos específicos da espécie suína que estão endemicamente disseminados em todo o mundo. O objetivo deste estudo foi investigar a infecção natural por estes picornavírus entéricos suínos em javalis (Sus scrofa scrofa) do estado do Paraná, Brasil e avaliar pecaris (Pecari tajacu e Tayassu pecari) como hospedeiros alternativos para estes vírus. Amostras fecais (n=36) de javalis assintomáticos (n=22) com idades de 2 a 7 meses (jovens, n=14) e 2 a 4 anos (adultos, n=8) e de pecaris (6 a 8 meses de idade, n=14) foram coletadas em um cativeiro e zoológico, res- Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric picornaviruses in wild boars (Sus scrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5'non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

et al. 2015

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Molecular epidemiology of Korean porcine sapeloviruses

et al. 2013

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To evaluate the prevalence and genetic diversity of porcine sapeloviruses (PSVs) in Korea, a total of 100 diarrhea fecal samples from pigs were analyzed by RT-PCR and nested PCR assays with primer pairs specific for the VP1 gene. Overall, 34 % of the diarrhea samples tested positive for PSV, and a high proportion of infections occurred along with a variety of other enteric viruses and bacteria. Genomic and phylogenetic analysis of the VP1 genes revealed pronounced genetic diversities between PSVs from Korean and elsewhere. Our results indicate that PSV infections are very common in Korean pigs with diarrhea. The infecting strains are genetically diverse.

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Analysis of full-length genomes of porcine teschovirus (PTV) and the effect of purifying selection on phylogenetic trees

et al. 2016

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To study the outcome of natural selection using phylogenetic trees, we analyzed full-length genome sequences of porcine teschovirus (PTV). PTV belongs to the family Picornaviridae and has a positive-stranded RNA genome, the replication of which is carried out by the error-prone viral RNA-dependent RNA polymerase. The viral RNA encodes a single polyprotein that is cleaved into structural (i.e., L, VP4, VP2, VP3 and VP1) and nonstructural proteins (i.e., 2A, 2B, 2C, 3A, 3B, and 3C). A high degree of genetic diversity was found based on the pairwise nucleotide distances and on the mean ratio of the number of nonsynonymous (dN) and synonymous (dS) substitutions (dN/dS) in the structural genes. Conversely, the diversity of the nonstructural genes was lower. The differences in genetic diversity between the structural and nonstructural genomic regions were likely due to strong purifying selection; consequently, the estimates of phylogenies were also discordant among these genes. In particular, maximum-likelihood and Bayesian methods generated short-branched trees when loci that are under strong purifying selection were used. These findings indicate that even in an RNA virus with an intrinsically high mutation rate, a strong purifying selection will curb genetic diversity and should be considered an important source of bias in future studies based on phylogenetic methods.

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The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011

Cited by 58 publications

References 22 publications

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

Molecular epidemiology of Korean porcine sapeloviruses

Analysis of full-length genomes of porcine teschovirus (PTV) and the effect of purifying selection on phylogenetic trees

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