The Survival Rate of Chick Embryo Influenced by Nicotine and Antioxidant Green Tea (Camellia Sinensis)

Abstract

Objectives: To study the effects of Nicotine on the survival rate of chick embryo and to evaluate the preventive role by the antioxidant green tea camellia sinensis.
 Study Design: Laboratory-based experimental study.
 Place and Duration of Study: Army Medical College, Rawalpindi, from Nov 2011 to Nov 2012.
 Methodology: A total of 75 Fayoumi fresh fertilized eggs were kept at Poultry Research Institute, Rawalpindi. Experimental solutions were injected to the eggs of four groups at forty-eight hours of incubation and the effect on the growth was recorded and compared with the control group. The control and experimental groups were observed to assess the effects of Nicotine and the role of antioxidant green tea Camellia sinensis on the survival rate and growth of chick embryo’s femur.
 Results: The control group (group-1) showed 100% survival rate of embryos. The experimental group-2 showed survival rate of 100% embryos and the group-3 and group-4 showed survival rate 50% and 70% of embryos, respectively.
 Conclusion: It was concluded that green tea decreases the oxidative stress caused by the Nicotine.

Effects of Nicotine on Chicken Embryo Development: A Review

“…The genotoxic and cytotoxic potential of Areca nut and smokeless tobacco were described [24]. In the study conducted by Shan et al [19,25] to observe the survival of nicotine-administered chick embryos observed a 90% survival rate in the control group and in that exposed to green tea extract, a 20% survival rate in the group administered nicotine, and a survival rate of 50% in the chicks exposed to both nicotine and green tea extract. The low survival rate of chicks exposed to nicotine could be a result of the hypoxic conditions created by nicotine-induced oxidative stress [25].…”