The sweet connection: Solving the riddle of multiple sugar‐binding fimbrial adhesins in <i>Escherichia coli</i>

Korea, Charalampia-Georgia; Ghigo, Jean‐Marc; Beloin, Christophe

doi:10.1002/bies.201000121

Cited by 72 publications

(60 citation statements)

References 107 publications

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“…A fragment of 1,588 bp was obtained by merging the two sequences, and blastn analysis indicated that it shares identity with cshB and faeD genes, encoding usher proteins involved in assembly of K88-related fimbriae. The prototype members of the K88 or F4 fimbriae were first identified in ETEC strains that infect pigs (16). Amino acid sequences derived from both genes did not contain the peptide sequence found in the AalE N terminus (TV NGDGGSV), suggesting that the identified novel putative usher protein is involved in the export of AalE to the bacterial surface.…”

Section: Resultsmentioning

confidence: 99%

Identification of Coli Surface Antigen 23, a Novel Adhesin of Enterotoxigenic Escherichia coli

et al. 2012

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g Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhea, mainly in developing countries. Although there are 25 different ETEC adhesins described in strains affecting humans, between 15% and 50% of the clinical isolates from different geographical regions are negative for these adhesins, suggesting that additional unidentified adhesion determinants might be present. Here, we report the discovery of Coli Surface Antigen 23 (CS23), a novel adhesin expressed by an ETEC serogroup O4 strain (ETEC 1766a), which was negative for the previously known ETEC adhesins, albeit it has the ability to adhere to Caco-2 cells. CS23 is encoded by an 8.8-kb locus which contains 9 open reading frames (ORFs), 7 of them sharing significant identity with genes required for assembly of K88-related fimbriae. This gene locus, named aal (adhesion-associated locus), is required for the adhesion ability of ETEC 1766a and was able to confer this adhesive phenotype to a nonadherent E. coli HB101 strain. The CS23 major structural subunit, AalE, shares limited identity with known pilin proteins, and it is more closely related to the CS13 pilin protein CshE, carried by human ETEC strains. Our data indicate that CS23 is a new member of the diverse adhesin repertoire used by ETEC strains.

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Section: Resultsmentioning

confidence: 99%

Identification of Coli Surface Antigen 23, a Novel Adhesin of Enterotoxigenic Escherichia coli

et al. 2012

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“…The main limitation of this approach is the multiplicity of structures involved in bacterial adhesion. However, one way to circumvent this issue is to use multivalent inhibitors linked to a scaffold of glycopolymers, glyconanoparticles that may permit inhibition of several adhesins at the same time (279)(280)(281).…”

Section: Targeting Biofilm Recalcitrance: Progress and Perspectivesmentioning

confidence: 99%

Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics

Lebeaux

Ghigo

Beloin

2014

Microbiol Mol Biol Rev

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1,050

862

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International audienceSurface-associated microbial communities, called biofilms, are present in all environments. Although biofilms play an important positive role in a variety of ecosystems, they also have many negative effects, including biofilm-related infections in medical settings. The ability of pathogenic biofilms to survive in the presence of high concentrations of antibiotics is called "recalcitrance" and is a characteristic property of the biofilm lifestyle, leading to treatment failure and infection recurrence. This review presents our current understanding of the molecular mechanisms of biofilm recalcitrance toward antibiotics and describes how recent progress has improved our capacity to design original and efficient strategies to prevent or eradicate biofilm-related infections

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“…Systematic studies have addressed this for bacteria such as E. coli and S. enterica, showing that while some fimbriae do not seem to have a role or to even be expressed under standard in vitro growth conditions, others are required at different levels to establish bacterial interactions with different biotic or abiotic surfaces and receptors, leading to cell adherence, colonization of different host tissues or environmental reservoirs, interactions between bacterial cells, biofilm formation, and pathogenesis (31,(34)(35)(36). Moreover, there is still much to learn about their specific expression patterns and the regulatory mechanisms coordinating their expression in response to the environment and the host (30).…”

Section: Discussionmentioning

confidence: 99%

Identification and Regulation of a Novel Citrobacter rodentium Gut Colonization Fimbria (Gcf)

et al. 2015

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The Gram-negative enteric bacterium Citrobacter rodentium is a natural mouse pathogen that has been extensively used as a surrogate model for studying the human pathogens enteropathogenic and enterohemorrhagic Escherichia coli. All three pathogens produce similar attaching and effacing (A/E) lesions in the intestinal epithelium. During infection, these bacteria employ surface structures called fimbriae to adhere and colonize the host intestinal epithelium. For C. rodentium, the roles of only a small number of its genome-carried fimbrial operons have been evaluated. Here, we report the identification of a novel C. rodentium colonization factor, called gut colonization fimbria (Gcf), which is encoded by a chaperone-usher fimbrial operon. A gcfA mutant shows a severe colonization defect within the first 10 days of infection. The gcf promoter is not active in C. rodentium under several in vitro growth conditions; however, it is readily expressed in a C. rodentium ⌬hns1 mutant lacking the closest ortholog of the Escherichia coli histone-like nucleoid structuring protein (H-NS) but not in mutants with deletion of the other four genes encoding H-NS homologs. H-NS binds to the regulatory region of gcf, further supporting its direct role as a repressor of the gcf promoter that starts transcription 158 bp upstream of the start codon of its first open reading frame. The gcf operon possesses interesting novel traits that open future opportunities to expand our knowledge of the structure, regulation, and function during infection of these important bacterial structures. IMPORTANCEFimbriae are surface bacterial structures implicated in a variety of biological processes. Some have been shown to play a critical role during host colonization and thus in disease. Pathogenic bacteria possess the genetic information for an assortment of fimbriae, but their function and regulation and the interplay between them have not been studied in detail. This work provides new insights into the function and regulation of a novel fimbria called Gcf that is important for early establishment of a successful infection by C. rodentium in mice, despite being poorly expressed under in vitro growth conditions. This discovery offers an opportunity to better understand the individual role and the regulatory mechanisms controlling the expression of specific fimbrial operons that are critical during infection. The natural mouse pathogen Citrobacter rodentium and the human pathogens enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) represent a family of enteric pathogenic bacteria that adhere to the host intestinal epithelial cells and inject effector proteins through a specialized type III secretion system (T3SS), subverting or hijacking several cell functions and leading to the formation of a distinctive histopathology known as the attaching and effacing (A/E) lesion (1, 2). These three pathogens produce similar lesions in the intestinal epithelium of their respective hosts, which are characterized by the intimate atta...

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The sweet connection: Solving the riddle of multiple sugar‐binding fimbrial adhesins in Escherichia coli

Cited by 72 publications

References 107 publications

Identification of Coli Surface Antigen 23, a Novel Adhesin of Enterotoxigenic Escherichia coli

Identification of Coli Surface Antigen 23, a Novel Adhesin of Enterotoxigenic Escherichia coli

Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics

Identification and Regulation of a Novel Citrobacter rodentium Gut Colonization Fimbria (Gcf)

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