The Synaptic Architecture of AMPA Receptors at the Cone Pedicle of the Primate Retina

Haverkamp, Silke; Grünert, Ulrike; Wässle, Heinz

doi:10.1523/jneurosci.21-07-02488.2001

Cited by 107 publications

(153 citation statements)

References 54 publications

Supporting

Mentioning

131

Contrasting

Order By: Relevance

“…PNA clusters at M-cones occupied a mean area of 5.2 Ϯ 1.1 m 2 (n ϭ 149), whereas those at S-cone pedicles had only a mean area of 3.4 Ϯ 0.9 m 2 (n ϭ 30; p Ͻ 0.001). For both markers, the labeled area at S-cone pedicles was significantly smaller than that at M-cones, consistent with the findings from primate (Ahnelt et al, 1990;Haverkamp et al, 2001a). This strengthens the view that S-cones are not merely variations of cones (as only defined by opsin expression) but a distinct photoreceptor type.…”

Section: Identifying Pedicles Of Mouse Cone Typessupporting

confidence: 88%

“…1C): M-cone pedicles (26.5 Ϯ 4.9 m 2 ; n ϭ 288) were on average larger than S-cone pedicles (22.4 Ϯ 4.3 m 2 ; n ϭ 25; p Ͻ 0.001). In line with this result and data from primate retina (Haverkamp et al, 2001a;Lee et al, 2005), we expected also a denser clustering of PNA at S-cones compared with M-cones. Because PNA colocalizes with mGluR6 at cone pedicles but not at rod spherules (Mataruga et al, 2007), this lectin can be used to label cone pedicle invaginations.…”

Section: Identifying Pedicles Of Mouse Cone Typessupporting

confidence: 87%

See 1 more Smart Citation

Chromatic Bipolar Cell Pathways in the Mouse Retina

Breuninger

Puller²,

Haverkamp³

et al. 2011

J. Neurosci.

134

View full text Add to dashboard Cite

Like most mammals, mice feature dichromatic color vision based on short (S) and middle (M) wavelength-sensitive cone types. It is thought that mammals share a retinal circuit that in dichromats compares S-and M-cone output to generate blue/green opponent signals, with bipolar cells (BCs) providing separate chromatic channels. Although S-cone-selective ON-BCs (type 9 in mouse) have been anatomically identified, little is known about their counterparts, the M-cone-selective OFF-BCs. Here, we characterized cone connectivity and light responses of selected mouse BC types using immunohistochemistry and electrophysiology. Our anatomical data indicate that four (types 2, 3a/b, and 4) of the five mouse OFF-BCs indiscriminately contact both cone types, whereas type 1 BCs avoid S-cones. Light responses showed that the chromatic tuning of the BCs strongly depended on their position along the dorsoventral axis because of the coexpression gradient of M-and S-opsin found in mice. In dorsal retina, where coexpression is low, most type 2 cells were green biased, with a fraction of cells (Ϸ14%) displaying strongly blue-biased responses, likely reflecting S-cone input. Type 1 cells were also green biased but did not comprise blue-biased "outliers," consistent with type 1 BCs avoiding S-cones. We therefore suggest that type 1 represents the green OFF pathway in mouse. In addition, we confirmed that type 9 BCs display blue-ON responses. In ventral retina, all BC types studied here displayed similar blue-biased responses, suggesting that color vision is hampered in ventral retina. In conclusion, our data support an antagonistically organized blue/green circuit as the common basis for mammalian dichromatic color vision.

show abstract

Section: Identifying Pedicles Of Mouse Cone Typessupporting

confidence: 88%

Section: Identifying Pedicles Of Mouse Cone Typessupporting

confidence: 87%

Chromatic Bipolar Cell Pathways in the Mouse Retina

Breuninger

Puller²,

Haverkamp³

et al. 2011

J. Neurosci.

134

View full text Add to dashboard Cite

show abstract

“…Furthermore, the number of photoreceptors as determined on semithin sections were comparable in WT and KO (WT: 191 Ϯ 17 per 100 m length, n ϭ 4; KO: 197 Ϯ 13 per 100 m length, n ϭ 5). The integrity of photoreceptor connectivity in the OPL was examined by labeling for bassoon [a marker of rod and cone ribbon synapses (Brandstätter et al, 1999)], peanut agglutinin [PNA; specific for cone pedicles (Haverkamp et al, 2001)], Go ␣ [specific for rod and ON-cone bipolar cells (Vardi, 1998)], and calbindin [a marker of horizontal cells (Peichl and González-Soriano, 1994)] (Fig. 3A).…”

Section: Nl2 Deficiency Does Not Affect the Core Architecture Of The mentioning

confidence: 99%

Neuroligin 2 Controls the Maturation of GABAergic Synapses and Information Processing in the Retina

Hoon¹,

Bauer²,

Fritschy³

et al. 2009

Journal of Neuroscience

View full text Add to dashboard Cite

In the present study, we investigated the role of Neuroligin 2 (NL2) in synaptic transmission and network function using the mouse retina as a model circuit. We show that NL2 is preferentially located at GABAergic rather than glycinergic or glutamatergic postsynapses. The absence of NL2 from the retina resulted in a severe reduction of GABA A receptor clustering, and in subtle alterations of the retinal circuitry. Light processing was impaired accordingly, and retinal ganglion cells, the output neurons of the retina, showed increased basal activity and altered coding of visual information. Together, our data indicate that NL2 is essential for the functional integrity of GABAergic signaling and as a consequence, for information processing in the retina.

show abstract

“…However, retinal neurons in transgenic mice with photoreceptor deficiencies have been shown to reorganize their synaptic contacts (Strettoi et al, 2002;Dick et al, 2003). To rule out the possibility that axon terminals in Cx36-deficient mice contact cone pedicles, we incubated retinas containing injected axon terminals with peanut agglutinin, which labels the base of the cone pedicles (Hack and Peichl, 1999;Haverkamp et al, 2001). Rotations of single scans revealed no overlap between axon terminal and cone pedicle labeling (Fig.…”

Section: How Do Cone Inputs Reach the Axon Terminal?mentioning

confidence: 99%

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Trümpler¹,

Dedek²,

Schubert³

et al. 2008

Journal of Neuroscience

View full text Add to dashboard Cite

Mammalian B-type horizontal cells make contact with both photoreceptor types: the dendrites contact cone photoreceptors, whereas the axon terminal processes contact rods. Despite their distinct synaptic contacts, horizontal cell somata and axon terminals receive a mixture of rod and cone inputs. Interaction of the two photoreceptor systems is essential for adaptation of photoreceptor sensitivity to different levels of background illumination, and horizontal cells play a key role in this adaptation. In this study, we used transgenic mouse lines to examine the contributions of rod and cone photoreceptor inputs to horizontal cell light responses in the mouse retina: rod signals were isolated by recording intracellularly from horizontal cells in a mouse lacking the cone cyclic nucleotide-gated channel, which lacks cone function, and cone signals were assessed using the rhodopsin knock-out mouse, which is a model for pure cone function. We found that both horizontal cell compartments receive a mixture of inputs from both photoreceptor types. To determine whether these inputs arrive via the long axon connecting the compartments or by way of rod-cone gap junctional coupling, we assessed the rod and cone contributions to horizontal cell somatic and axon terminal light responses in the connexin36-deficient mouse retina, which lacks rodcone coupling. Our results confirm that rods and cones are coupled by connexin36, and suggest that signal transmission along the axon is unidirectional: signals are passed from horizontal cell soma to axon terminal but not from axon terminal to soma.

show abstract

The Synaptic Architecture of AMPA Receptors at the Cone Pedicle of the Primate Retina

Cited by 107 publications

References 54 publications

Chromatic Bipolar Cell Pathways in the Mouse Retina

Chromatic Bipolar Cell Pathways in the Mouse Retina

Neuroligin 2 Controls the Maturation of GABAergic Synapses and Information Processing in the Retina

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Contact Info

Product

Resources

About