The synergistic effects of microfracture, perforated decalcified cortical bone matrix and adenovirus-bone morphogenetic protein-4 in cartilage defect repair

A New Source of Mesenchymal Stem Cells for Articular Cartilage Repair

“…The DBM scaffolds were prepared according to the protocols of previous studies. 42 Cortical bone was obtained from the midshafts of the femur and tibia of adult New Zealand White rabbits. First, removing the soft tissue before storage at 270°C until further use, DBM was subjected to the following procedures: (1) demineralization in 0.6 mol/L hydrochloric acid at 4°C for 7 days, (2) defatting under 1:1 (vol/vol) methanol/chloroform solution for 24 hours, and (3) deproteinization with 3% hydrogen peroxide for 4 hours.…”

Articular Cartilage Tissue Engineering

“…The damaged cartilage is first removed down to the calcified zone to expose healthy adjacent tissue. Other improvements to the technique include the addition of a BMP-4 carrier, which showed more rapid repair [705]. Blood then fills the defect, resulting in a fibrin clot that initiates a healing response as described in Chapter 2 The repair tissue is thus fibrocartilaginous in nature, with inferior material properties as compared to healthy articular cartilage.…”

Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering

“…The commercial human cortical cancellous bone was demineralized by soaking in ethylene diamine tetraacetic acid (EDTA, 0.5 M , pH = 8.3) according to a previously mentioned method to obtain the natural 3D DBS (see related results in Supporting Information Fig. S1) . At last, the human decalcified bone was made into a cylinder 2 mm in diameter and 4 mm in height by a corneal trephine [Fig.…”

“…This peptide was then covalently conjugated onto PCL electrospun meshes to evaluate its TE applications. Furthermore, we conjugated this peptide onto the surface of a natural, porous scaffold material, and human decalcified bone scaffold (hDBS), which we found is beneficial for native hyaline articular cartilage regeneration . We also investigated the adhesion of SMSCs onto these two fundamentally different materials after surface modification by SMSCs‐affinity peptide L7.…”