The synergistic regulation of chondrogenesis by collagen-based hydrogels and cell co-culture

Functional injectable hydrogel with spatiotemporal sequential release for recruitment of endogenous stem cells and in situ cartilage regeneration

“…A quantitative analysis of DNA content allowed a precise assessment of the chondrogenic potential of cells in different hydrogels by assessing the average sGAG production per cell. 38 Cells in Col@KGN MPs and Col-Apt@KGN MPs groups produced more sGAG than that in Col and Col-Apt groups on 14 and 21 days, and there was a time-dependent increase in sGAG contents (Fig. 6D).…”

“…The methods of isolating and encapsulating BMSCs were in accord with previously published methods. 38 In brief, long bones were harvested from sacrificed newborn New Zealand white rabbits. Under sterile conditions, the cartilage was excised, and the BMSCs in the bone marrow cavity were repeatedly washed with α-MEM medium containing 20% FBS and 1% PS with a syringe.…”

Components and physical properties of hydrogels modulate inflammatory response and cartilage repair

“…According to the previously published method, bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from newborn New Zealand white rabbits. 19 The second passaged BMSCs were applied in this study.…”

Anatomy, molecular structures, and hyaluronic acid – Gelatin injectable hydrogels as a therapeutic alternative for hyaline cartilage recovery: A review

“…This kind of substitutes provide a doble function. On the one hand, chondrocytes, which have lower proliferation capacity, increase the cartilaginous matrix production, while MSCs not only secrete chondrogenic markers, but also proliferate enriching the ECM components 147 . The culture of MSCs as spheroids has been proposed as a possible solution to avoid cell dedifferentiation.…”

“…Collagen II in the GelMA/ECM and GelMA/ ECM-PFS groups was significantly higher 140 (Continues) secrete chondrogenic markers, but also proliferate enriching the ECM components. 147 The culture of MSCs as spheroids has been proposed as a possible solution to avoid cell dedifferentiation. For this reason, spheroids of MSCs have been cultured on tyramine-modified hyaluronic acid and collagen type I hydrogels, showing a 3D biomimetic environment that promotes not only chondrogenic differentiation, but also cell-cell interaction and a good diffusion of nutrients and oxygen similar to native tissue.…”