The synthesis of oligosaccharides by the reversed hydrolysis reaction of ?-glucosidase at high substrate concentration and at high temperature

Ajisaka, Katsumi; Nishida, Hiroko; Fujimoto, Hiroshi

doi:10.1007/bf01027157

Cited by 95 publications

(29 citation statements)

References 7 publications

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“…Hansson et al (2001) reported that GOS formation with b-galactosidase was more of a kinetically controlled transgalactosylation reaction rather than reversed hydrolysis, which is under thermodynamic control. Reversed hydrolysis or condensation may occur at high concentrations of mono-saccharides upon prolonged incubations, as reported for other glycosidases (Ajisaka et al, 1987;Nikolov et al, 1989;Pestlin et al, 1997). However, no appreciable amount of di-or oligosaccharides could be formed from glucose and/or galactose by A. oryzae lactase when the monosaccharides were present as the sole substrates at 50 g/L during the course of 5 h (data not shown).…”

Section: Effects Of Galactose and Glucosementioning

confidence: 67%

“…It has been shown that allolactose, a disaccharide, can be synthesized from galactose and glucose, although the product yield was only less than 10% (Ajisaka and Fujimoto, 1989;Ajisaka et al, 1987). Galactose is known as a competitive inhibitor to the lactose hydrolysis reaction (Bakken et al, 1991;Ogushi et al, 1980;Park et al, 1979;Portaccio et al, 1998;Shukla and Chaplin, 1993;Yang and Okos, 1989).…”

Section: Effects Of Galactose and Glucosementioning

confidence: 97%

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Production of galacto‐oligosaccharides from lactose by Aspergillus oryzae β‐galactosidase immobilized on cotton cloth

Albayrak

Yang²

2001

Biotech & Bioengineering

174

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The production of galacto-oligosaccharides (GOS) from lactose by A. oryzae beta-galactosidase immobilized on cotton cloth was studied. The total amounts and types of GOS produced were mainly affected by the initial lactose concentration in the reaction media. In general, more and larger GOS can be produced with higher initial lactose concentrations. A maximum GOS production of 27% (w/w) of initial lactose was achieved at 50% lactose conversion with 500 g/L of initial lactose concentration. Tri-saccharides were the major types of GOS formed, accounting for more than 70% of the total GOS produced in the reactions. Temperature and pH affected the reaction rate, but did not result in any changes in GOS formation. The presence of galactose and glucose at the concentrations encountered near maximum GOS greatly inhibited the reactions and reduced GOS yield by as much as 15%. The cotton cloth as the support matrix for enzyme immobilization did not affect the GOS formation characteristics of the enzyme, suggesting no diffusion limitation in the enzyme carrier. The thermal stability of the enzyme increased approximately 25-fold upon immobilization on cotton cloth. The half-life for the immobilized enzyme on cotton cloth was more than 1 year at 40 degrees C and 48 days at 50 degrees C. Stable, continuous operation in a plugflow reactor was demonstrated for 2 weeks without any apparent problem. A maximum GOS production of 21 and 26% (w/w) of total sugars was attained with a feed solution containing 200 and 400 g/L of lactose, respectively, at pH 4.5 and 40 degrees C. The corresponding reactor productivities were 80 and 106 g/L/h, respectively, which are at least several-fold higher than those previously reported.

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Section: Effects Of Galactose and Glucosementioning

confidence: 67%

Section: Effects Of Galactose and Glucosementioning

confidence: 97%

Production of galacto‐oligosaccharides from lactose by Aspergillus oryzae β‐galactosidase immobilized on cotton cloth

Albayrak

Yang²

2001

Biotech & Bioengineering

174

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“…Interestingly, the optimum pH for the synthesis of trisaccharide was 7.0 whereas the optimum pH for the hydrolysis of cellobiose was 5.0 ( Figure 1). In contrast, Ajisaka et al (1987) reported that 13-glucosidase from almond catalysed the hydrolysis and synthesis reactions at optimum pH of 5.5 and 4.0, respectively. …”

Section: Resultsmentioning

confidence: 98%

“…Despite these advantages, the enzymatic synthesis of oligosaccharides is restricted mainly to disaccharidcs (Ajisaka et al, 1987). The synthesis of higher oligosaccharides by transglycosylation has been demonstrated only in a laboratory scale (Wood & McCrae, 1982;Bhat et al, 1990;.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ?-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose

et al. 1994

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Purified 13-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15 -20 % of the initial substrate concentration. These results suggested that 13-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.

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“…Most of them are not competitive from a commercial point of view, even if their potential interest was demonstrated at laboratory scale. The action of a β -glucosidase on concentrated glucose solutions (70%, w/w) results in the synthesis of β -glucooligosaccharides [142] , mainly di -and trisaccharides containing β -1,6 (gentiobiose and gentiotriose) and β -1,4 (cellobiose and cellotriose) linkages.…”

Section: Resistant Starchmentioning

confidence: 99%

Industrial Biotechnology in the Food and Feed Sector

Monsan¹,

O’Donohue²

2010

Industrial Biotechnology

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The synthesis of oligosaccharides by the reversed hydrolysis reaction of ?-glucosidase at high substrate concentration and at high temperature

Cited by 95 publications

References 7 publications

Production of galacto‐oligosaccharides from lactose by Aspergillus oryzae β‐galactosidase immobilized on cotton cloth

Production of galacto‐oligosaccharides from lactose by Aspergillus oryzae β‐galactosidase immobilized on cotton cloth

Optimization of ?-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose

Industrial Biotechnology in the Food and Feed Sector

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