The Taste Bud and Its Innervation in the Rat as Studied by Immunohistochemistry for PGP 9.5.

Kanazawa, Hiroaki; Souma, Yoshie

doi:10.1679/aohc.59.357

Cited by 43 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Taste bud cells were distinguished into two groups, the intragemmal cells and the perigemmal cells, by the variety of keratin [7], and furthermore, morphologically subdivided into 4 types [6,12]. In our study, it was not able to distinguish the cell types of the taste bud stained by lectins, but the taste bud cells stained by BSL-I were speculated as the type I cells from their population, shape and localization in all taste buds examined.…”

Section: Discussioncontrasting

confidence: 68%

Glycoconjugate in Rat Taste Buds.

Kanô

Ube

Taniguchi

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The taste buds of the fungiform papillae, circumvallate papilla, foliate papillae, soft palate and epiglottis of the rat oral cavity were examined by lectin histochemistry to elucidate the relationships between expression of glycoconjugates and innervation. Seven out of 21 lectins showed moderate to intense staining in at least more than one taste bud. They were succinylated wheat germ agglutinin (s-WGA), Dolichos biflorus agglutinin (DBA), Bandeiraea simplicifolia lectin-I (BSL-I), Ricinus communis agglutinin-I (RCA-I), peanut agglutinin (PNA), Ulex europaeus agglutinin-I (UEA-I) and Phaseolus vulgaris agglutinin-L (PHA-L). UEA-I and BSL-I showed moderate to intense staining in all of the taste buds examined. They strongly stained the taste buds of the epiglottis, which are innervated by the cranial nerve X. UEA-I intensely stained the taste buds of the fungiform papillae and soft palate, both of which are innervated by the cranial nerve VII. The taste buds of circumvallate papilla and foliate papillae were innervated by the cranial nerve IX and strongly stained by BSL-I. Thus, UEA-I and BSL-I binding glycoconjugates, probably α-linked fucose and α-D-galactose, respectively, might be specific for taste buds. Although the expression of these glycoconjugates would be related to the innervation of the cranial nerve X, the differential expression of α-linked fucose and α-D-galactose might be related to the innervation of the cranial nerve VII and IX, respectively. KEY WORDS: glycoconjugate, innervation, lectin-histochemistry, rat, taste bud.The gustatory sense is one of four special senses in mammals. The gustatory sense is transmitted by the taste buds distributed in the oral cavity and laryngopharynx. The taste buds, composed of 40-70 cells with different cell types, have a complex structure and access to the oral cavity via the taste pore [2,10,12,17,23]. They can catch chemical materials in the solvent and transmit the signals to the central nervous system. In mammals, the taste buds are observed in fungiform papillae, circumvallate papillae, foliate papillae, soft palate and epiglottis where individual taste bud is innervated by only one of three kinds of the cranial nerves, VII, IX, and X. Taste buds of fungiform papillae, anterior region of foliate papillae and soft palate were innervated by chorda tympani of the cranial nerve VII [14]. Taste buds of circumvallate papilla and posterior region of foliate papillae were innervated by the cranial nerve IX and those of epiglottis were innervated by the cranial nerve X in the rat [14]. These cranial nerves have crucial roles for the development, maintenance and regeneration of the taste buds in the rat [3,4,11,13,15,24].The glycoconjugates on the surface of taste bud cells were involved in chemosensory regulation in olfactory cilia of the channel catfish [5]. The carbohydrate components in the taste buds of circumvallate papillae were detected by lectin histochemistry in the Syrian hamster [16]. In the tongue of various vertebrates, Witt et al. showed ma...

show abstract

Section: Discussioncontrasting

confidence: 68%

Glycoconjugate in Rat Taste Buds.

Kanô

Ube

Taniguchi

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Only PGP9.5 + pixels that (1) fell within the K8 + taste bud ROI and (2) were clustered in groups of ≥5 pixels were tallied as intragemmal PGP9.5 + pixels. Importantly, although some taste cells are PGP9.5 + (Kanazawa and Yoshie, 1996), these cells were rare and significantly dimmer, in terms of immunolabeling, than nerve fibers. Therefore, MATLAB thresholding eliminated the taste cell signal (confirmed by double labeling with K8; data not shown), allowing us to isolate and analyze the brighter PGP9.5 + neurites.…”

Section: Image Acquisition and Analysismentioning

confidence: 92%

“…The mechanosensory filiform papillae of the anterior tongue, the epithelial cells of fungiform papillae, as well as taste buds per se are innervated by somatosensory fibers emanating from the trigeminal ganglia (Finger and Simon, 2000), and trigeminal fibers support taste buds in cross-innervation studies (Kinnman and Aldskogius, 1988). To test if somatosensory fibers innervate ectopic taste buds in SHH-YFPcKI + non-taste epithelium, we used PGP9.5 (UCHL1), a general marker of somatosensory and gustatory nerve fibers (Kanazawa and Yoshie, 1996), to examine both intragemmal (within buds) and perigemmal (surrounding buds) innervation (Fig. 5C) of endogenous and ectopic taste buds.…”

Section: Shh Induction Of Ectopic Taste Buds Is Independent Of Taste mentioning

confidence: 99%

Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium

et al. 2014

View full text Add to dashboard Cite

Taste buds are assemblies of elongated epithelial cells, which are innervated by gustatory nerves that transmit taste information to the brain stem. Taste cells are continuously renewed throughout life via proliferation of epithelial progenitors, but the molecular regulation of this process remains unknown. During embryogenesis, sonic hedgehog (SHH) negatively regulates taste bud patterning, such that inhibition of SHH causes the formation of more and larger taste bud primordia, including in regions of the tongue normally devoid of taste buds. Here, using a Cre-lox system to drive constitutive expression of SHH, we identify the effects of SHH on the lingual epithelium of adult mice. We show that misexpression of SHH transforms lingual epithelial cell fate, such that daughter cells of lingual epithelial progenitors form cell type-replete, onion-shaped taste buds, rather than non-taste, pseudostratified epithelium. These SHH-induced ectopic taste buds are found in regions of the adult tongue previously thought incapable of generating taste organs. The ectopic buds are composed of all taste cell types, including support cells and detectors of sweet, bitter, umami, salt and sour, and recapitulate the molecular differentiation process of endogenous taste buds. In contrast to the well-established nerve dependence of endogenous taste buds, however, ectopic taste buds form independently of both gustatory and somatosensory innervation. As innervation is required for SHH expression by endogenous taste buds, our data suggest that SHH can replace the need for innervation to drive the entire program of taste bud differentiation.

show abstract

“…Recently, KANAZAWA and YOSHIE (1996) demonstrated by electron microscopy that PGP 9.5 immunoreactivity was localized in the type III or gustatory cell. Based on the present findings that 1) the amount of PGP 9.5 immunoreactivity was considerably higher than that of 5HT, 2) the PGP 9.5 immunoreactive cells outnumbered the 5HT immunoreactive cells, and 3) 5HT immunoreactive cells outnumbered the PGP 9.5 immunonegative cells, it is reasonable to suggest that some of 5HT immunoreactive cells would be also PGP 9.5 immunoreactive in guinea pig vallate taste buds.…”

Section: Serotonin (5ht)mentioning

confidence: 99%

Immunohistochemical Studies on Protein Gene Product 9.5, Serotonin and Neuropeptides in Vallate Taste Buds and Related Nerves of the Guinea Pig.

1996

Archives of Histology and Cytology

View full text Add to dashboard Cite

The Taste Bud and Its Innervation in the Rat as Studied by Immunohistochemistry for PGP 9.5.

Cited by 43 publications

References 41 publications

Glycoconjugate in Rat Taste Buds.

Glycoconjugate in Rat Taste Buds.

Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium

Immunohistochemical Studies on Protein Gene Product 9.5, Serotonin and Neuropeptides in Vallate Taste Buds and Related Nerves of the Guinea Pig.

Contact Info

Product

Resources

About