The Technique of Thyroid Cartilage Scaffold Support Formation for Extrusion-Based Bioprinting

Abstract

During biofabrication, a tissue scaffold may require temporary support. The aim of this study was to develop an approach of human thyroid cartilage scaffold temporal support formation. The scaffold 3D-model was based on DICOM images. XY plane projections were used to form scaffold supporting part. To verify the technique, collagen hydrogel was chosen as the main scaffold component. Gelatin was applied for the supporting part. To test the applicability of the approach, a model of thyroid cartilage scaffold with the support was printed. The scaffold corresponded to a given model, although some discrepancy in geometry was observed during verification by computed tomography.

Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

“…The bioink was based on sterile type I pig atelocollagen. All the procedures with the material were in accordance with the previously described method [ 65 ]. Briefly, on the day of the experiment, the cells were removed from a culture flask with trypsin-EDTA solution.…”

Bioprinting of Cartilage with Bioink Based on High-Concentration Collagen and Chondrocytes

“…There is a significant number of published data on restoring articular cartilage [ 4 ], the trachea [ 5 ], intervertebral disc [ 6 ], auricle [ 7 ], and thyroid cartilage [ 8 ]. Collagen is the most applied biomaterial for tissue-engineered structures (scaffolds) [ 9 ].…”

“…The following strategies can be applied to improve the mechanical properties: (1) supplementing collagen with cross-linking agents (for example, riboflavin [ 17 ], or methacrylamide and methacrylate groups [ 18 ]); (2) creating multicomponent bioink [ 12 , 19 , 20 ]; or (3) using higher collagen concentrations. The applicability of collagen-based hydrogels with a concentration as high as 4% has been shown in a few studies [ 8 , 21 ].…”

Application of 3-dimensional printing implants for bone tumors

“…Skin, vessels, cartilage, and bone tissue are common targets in orthopedics. 5,[44][45][46][47][48][49] In addition, bioprinting has advantages over traditional methods for 3D cell culture for creating and utilizing a tumor model. 8) Case presentation 1.…”

“…Tissue engineering in orthopedic surgery is currently experimental. Skin, vessels, cartilage, and bone tissue are common targets in orthopedics [ 5 , 44 - 49 ]. In addition, bioprinting has advantages over traditional methods for 3D cell culture for creating and utilizing a tumor model [ 8 ].…”