The TEM characterization of the lamellar structure of osteoporotic human trabecular bone

Rubin, Matthew A.; Jasiuk, Iwona

doi:10.1016/j.micron.2005.07.010

Cited by 46 publications

(24 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TEM has also allowed local qualitative assessment of the ultrastructure arrangement of healthy and osteoporotic bone [233,234], but in two dimensions only ( figure 13). Furthermore, TEM has been applied to make important contributions to what we know about bone structure at very small scales, through investigations of the shape and size of bone crystals [14,235], their spatial relationship and arrangement in relation to the collagen fibrils [175,236] or features of the collagen fibrils such as the approximately 67 nm D-spacing [7] (figure 13).…”

Section: Electron-based Imaging Techniques 2331 Transmission Elecmentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

View full text Add to dashboard Cite

Bone's remarkable mechanical properties are a result of its hierarchical structure. The mineralized collagen fibrils, made up of collagen fibrils and crystal platelets, are bone's building blocks at an ultrastructural level. The organization of bone's ultrastructure with respect to the orientation and arrangement of mineralized collagen fibrils has been the matter of numerous studies based on a variety of imaging techniques in the past decades. These techniques either exploit physical principles, such as polarization, diffraction or scattering to examine bone ultrastructure orientation and arrangement, or directly image the fibrils at the sub-micrometre scale. They make use of diverse probes such as visible light, X-rays and electrons at different scales, from centimetres down to nanometres. They allow imaging of bone sections or surfaces in two dimensions or investigating bone tissue truly in three dimensions, in vivo or ex vivo, and sometimes in combination with in situ mechanical experiments. The purpose of this review is to summarize and discuss this broad range of imaging techniques and the different modalities of their use, in order to discuss their advantages and limitations for the assessment of bone ultrastructure organization with respect to the orientation and arrangement of mineralized collagen fibrils.

show abstract

Section: Electron-based Imaging Techniques 2331 Transmission Elecmentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

View full text Add to dashboard Cite

show abstract

“…Their objective was to determine if the fractal analysis of texture was able to distinguish osteoporotic fracture groups, either in vertebrae, hip or wrist fractures. Rubin and Jasiuk [14] characterized the lamellar structure of osteoporotic human trabecular bone using TEM.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of some physico-chemical characteristics of osteoporotic and normal human femur heads

Sastry

Chandrsekaran

Sundaraseelan

et al. 2007

Clinical Biochemistry

View full text Add to dashboard Cite

“…More recently it has been used to investigate tethering fibres between osteocytes and the PCM on a nanoscopic scale (Fig. 7) (You et al, 2004;McNamara et al, 2009) and the organisation of collagen and crystals in the bone matrix (Rubin and Jasiuk, 2005). The 2D nature of TEM imaging has been somewhat overcome by the introduction of serial section TEM, TEM tomography and ultra-high voltage EM (UHVEM).…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease

Goggin

Zygalakis

Oreffo³

et al. 2016

eCM

View full text Add to dashboard Cite

Osteocytes are involved in mechanosensation and mechanotransduction in bone and hence, are key to bone adaptation in response to development, ageing and disease. Thus, detailed knowledge of the three-dimensional (3D) structure of the osteocyte network (ON) and the surrounding lacuno-canalicular network (LCN) is essential. Enhanced understanding of the ON&LCN will contribute to a better understanding of bone mechanics on cellular and sub-cellular scales, for instance through improved computational models of bone mechanotransduction. Until now, the location of the ON within the hard bone matrix and the sub-µm dimensions of the ON&LCN have posed significant challenges for 3D imaging. This review identifies relevant microstructural phenotypes of the ON&LCN in health and disease and summarises how light microscopy, electron microscopy and X-ray imaging techniques have been used in studies of osteocyte anatomy, pathology and mechanobiology to date. In this review, we assess the requirements for ON&LCN imaging and examine the state of the art in the fields of imaging and computational modelling as well as recent advances in high-resolution 3D imaging. Suggestions for future investigations using volume electron microscopy are indicated and we present new data on the ON&LCN using serial block-face scanning electron microscopy. A correlative approach using these high-resolution 3D imaging techniques in conjunction with in silico modelling in bone mechanobiology will increase understanding of osteocyte function and, ultimately, lead to improved pathways for diagnosis and treatment of bone diseases such as osteoporosis.Keywords: Osteocyte, 3D imaging, microscopy, b i o m e c h a n i c s , l a c u n o -c a n a l i c u l a r n e t w o r k , mechanobiology, mechanosensation, mechanotransduction, osteoporosis. IntroductionThrough bone adaptation, the skeleton adapts continuously to changed mechanical loading patterns due to development, growth, ageing, disease, disuse or exercise, by removing existing and adding new bone tissue. It has been recognised that osteocytes are the key cells which orchestrate bone adaptation (Tatsumi et al., 2007). Osteocytes are ovoid cells approximately 10 µm long, surrounded by a pericellular matrix (PCM). The osteocytes and their processes form the osteocyte network (ON), which is housed within the lacuno-canalicular network (LCN), a system of voids and channels in the calcified bone matrix (Fig. 1). Neve et al., 2012). Knowledge of the three-dimensional (3D) structure of the ON&LCN would inform conclusions about the function and malfunction of osteocytes for different bone states in development, ageing, disease, disuse or exercise. More specifically, enhanced knowledge would improve the predictive power and accuracy of computational models, which attempt to elucidate the mechanisms of mechanotransduction using 3D geometries for the ON&LCN. Recent finite element and fluid-structure interaction models have used relatively low resolution image data and made a priori assumptions about the ...

show abstract

The TEM characterization of the lamellar structure of osteoporotic human trabecular bone

Cited by 46 publications

References 33 publications

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Comparative study of some physico-chemical characteristics of osteoporotic and normal human femur heads

High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease

Contact Info

Product

Resources

About