The temporary dynamics of inflammation-related genes expression under tuftsin analog Selank action

Kolomin, Timur; Morozova, Marina; Волкова, А В; Шадрина, М. И.; Andreeva, L. A.; Slominsky, Petr; Limborska, Svetlana A.; Myasoedov, N. F.

doi:10.1016/j.molimm.2013.11.002

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…Полученные данные свидетельствуют о наличие у изучаемых соединений иммуномодулирующих свойств. Важно отметить, что влияние глипролинов на иммунную систему наблюдали и ранее [19,20,21], но в данном аспекте действие пептидов изучается впервые.…”

Section: Discussionunclassified

Glyprolines as Modulators of Immunoreactivity Within Conditions of “Social” Stress

Самотруева

Ясенявская

Bashkina

et al. 2019

Farm. farmakol. (Pâtigorsk)

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The most important direction in the development of modern medical science is the study of protective, compensatory and pathological reactions of the organism that occur in response to various stress factors.The aim of the study is the subsequent development of methods for pharmacological correction of these reactions. The remedies for the correction of stress-induced immunity disorders are represented by the glyprolin group – the Selank drug and the Pro-Gly-Pro peptide compound – and are of particular interest. The aim of the experiment was to study the immunomodulating effect of glyprolines on the basis of the “social stress” model.Materials and methods. The experiment was performed on non-linear male rats aged 6-8 months. A model of a sensor contact was used as a model of the experimental “social stress”. The animals were divided into groups (n = 10): a “control” group was represented by individuals with aggressive and submissive types of behavior, formed within the conditions of the experimental “social stress” for 20 days; and 2 experimental groups in which the animals were intraperitoneally administered Selank (100 µg/kg) and Pro-Gly-Pro (100 µg/kg) against the background of the experimental “social” stress once a day for 20 days. A functional activity of the immune system was studied on the basis of standard immunopharmacological tests: a delayed-type hypersensitivity test (DTH test), a direct agglutination test (DAT), a latex test for studying the Neutrophil phagocytic rate of peripheral blood, and the essessment of the leucogram.Results. It has been established, that within the conditions of the “social” stress, the changes in the immune response are multidirectional. That fact confirms the theory of “the immune disbalance” caused by the action of stressors. As a result of studying the effect of glyprolines within the conditions of “the social stress”, Selank and Pro-Gly-Pro proved to be effective immunocorrectors, restoring cellular and humoral immunogenesis reactions as well as the phagocytic activity of neutrophils and leucogram indices.Сonclusion. The carried out study expands understanding of the immunoreaction pathogenesis within the stress-induced conditions in order to further develop a pharmacological strategy for correcting the revealed disorders through the substances of the neuropeptide structure.

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Section: Discussionunclassified

Glyprolines as Modulators of Immunoreactivity Within Conditions of “Social” Stress

Самотруева

Ясенявская

Bashkina

et al. 2019

Farm. farmakol. (Pâtigorsk)

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“…These fragments of Selank produced the same effect as the intact Selank. These properties were expressed in the GP dipeptide, so the authors suggested that GP might be a pharmacophore of the action of Selank on the immune system 32‐34 …”

Section: Analysis Of Gene Expression Under the Action Of Peptides: Frmentioning

confidence: 99%

Pharmacotranscriptomics of peptide drugs with neuroprotective properties

Dergunova

Filippenkov

Limborska

et al. 2020

Medicinal Research Reviews

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Here we present a review of studies on the effects of peptides with neuroprotective properties on gene transcription in nerve cells. The few published works in this area clearly demonstrate massive changes in cell transcriptomes induced by peptides under normal conditions and under conditions of experimental brain ischemia. These changes significantly affect signaling and metabolic pathways, affecting various body systems and confirming the multiple target actions of peptides. The importance of noncoding RNAs in the regulation of these processes is shown, and we discuss the prospects of research for determining the main mechanisms of peptide regulation, which is necessary for the further development of drugs with targeted neuroprotective effects.

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“…Previously, it was shown that Selank causes a marked change in the expression of genes involved in inflammatory processes in the hippocampus and spleen of rodents (Kolomin et al, 2010 , 2011 , 2014 ). Our results have confirmed at the molecular level that the clinical effects observed after the introduction of Selank are related to its antiviral activity (Ershov et al, 2009 ; Andreeva et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

et al. 2017

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Clinical studies have shown that Selank had an anxiolytic effect comparable to that of classical benzodiazepine drugs, which can enhance the inhibitory effect of GABA by allosteric modulation of GABAA receptors. These data suggest that the molecular mechanism of the effect of Selank may also be related to its ability to affect the performance of the GABAergic system. To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method. As test substances, in addition to Selank, we selected the major GABAA receptor ligand, GABA, the atypical antipsychotic, olanzapine, and combinations of these compounds (Selank and GABA; Selank and olanzapine). We found no changes in the mRNA levels of the genes studied under the effect of Selank. The combined effect of GABA and Selank led to nearly complete suppression of changes in expression of genes in which mRNA levels changed under the effect of GABA. When Selank was used in conjunction with olanzapine, the expression alterations of more genes were observed compared with olanzapine alone. The data obtained indicate that Selank has no direct effect on the mRNA levels of the GABAergic system genes in neuroblastoma IMR-32 cells. At the same time, our results partially confirm the hypothesis that the peptide may affect the interaction of GABA with GABAA receptors. Our data also suggest that Selank may enhance the effect of olanzapine on the expression of the genes studied.

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The temporary dynamics of inflammation-related genes expression under tuftsin analog Selank action

Cited by 9 publications

References 16 publications

Glyprolines as Modulators of Immunoreactivity Within Conditions of “Social” Stress

Glyprolines as Modulators of Immunoreactivity Within Conditions of “Social” Stress

Pharmacotranscriptomics of peptide drugs with neuroprotective properties

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

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