The teratogenic effects of the ethanolic extract of green tea (Camellia sinensis) in the larval stage of zebrafish (Danio rerio)

Background: Polyherbal extracts, composed of Yacon (Smallanthus sonchifolius), Bay leaves (Syzygium polyantum), Stevia (Stevia rebaudiana), and tea leaves (Camellia sinensis), are known for their anti-diabetic properties. To be developed as standardized herbal medicines, these formulations must meet safety standards, including teratogenicity testing. Aim: This study aims to assess the teratogenic effects of an ethanolic extract of this anti-diabetic polyherbal formulation in pregnant adult female mice. Methods: An in vivo study was conducted using pregnant female mice. The polyherbal extract, suspended in 0.1% Na-CMC, was administered at doses of 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW. The extract was given during the organogenesis phase, from day 6 to day 15 of gestation. The mice were sacrificed on day 17 to evaluate fetal development. Results: Results indicated a dose-dependent delay in fetal bone ossification with increasing extract doses. However, there were no significant effects on physical fetal development, nor were there abnormalities observed in the soft tissues or skeletal structures of the fetuses. Conclusion: The administration of the polyherbal extract in pregnant female mice caused a delay in fetal ossification but did not result in teratogenic effects, such as physical deformities or soft tissue abnormalities. Further studies are needed to assess long-term safety and potential developmental impacts at varying doses.

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The teratogenic effects of the ethanolic extract of green tea (Camellia sinensis) in the larval stage of zebrafish (Danio rerio)

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Microencapsulation of green tea (Camellia sinensis) phenolic extract: Physical-chemical characterization, antimicrobial and toxicological properties

Microencapsulation of green tea (Camellia sinensis) phenolic extract: Physical-chemical characterization, antimicrobial and toxicological properties

Teratogenicity <i>In Vivo</i> Experiment of Antidiabetic Polyherbal (<i>Smallanthus sonchifolius, Syzygium poloyantum, Stevia rebaudiana, Camellia sinensis</i>) in Pregnant Mice

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