We describe a new frog, Pristimantis mutabilis sp. nov., from the Andes of Ecuador. Individuals of the new species are remarkable for their ability to change skin texture from tuberculate to almost smooth in a few minutes, being the first documented amphibian species to show such dramatic phenotypic plasticity. The new taxon is assigned to the P. myersi group. It differs from other members of its group by body size (adult males 17.2-17.4 mm; adult females 20.9-23.2 mm), arboreal habitat, and red flash coloration in females. We document three call types for the new species, which differ through their number of notes and amplitude peaks. The three types are pulsed calls that share a dominant frequency of 3186.9-3445.3 Hz. Surprisingly, we also document similar skin texture plasticity in species (P. sobetes) from a different species group, suggesting that this ability might be more common than previously thought. The discovery of these variable species poses challenges to amphibian taxonomists and field biologists, who have traditionally used skin texture and presence/absence of tubercles as important discrete traits in diagnosing and identifying species. Reciprocal monophyly and genetic distances also support the validity of the new species, as it has distances of 15.1-16.3% (12S) and 16.4-18.6% (16S) from the most similar species, Pristimantis verecundus. Additionally, each of the two known populations of Pristimantis mutabilis are reciprocally monophyletic and exhibit a high genetic distance between them (5.0-6.5%). This pattern is best explained by the presence of a dry valley (Guayllabamba River) that seems to be acting as a dispersal barrier. Scenario 1: double origin Scenario 2: single origin P. buckleyi (KU 217836 P. sp. "mashpi" (MZUTI 635) P. surdus (KU 177847) P. hectus (UVC 15843) P. cf. verecundus (QCAZ 12410) P. cf. curtipes (KU 217869 P. sobetes (MZUTI 447) P. thymelensis (TNHC-GDC 14370) P. jubatus (UVC 15919) P. jubatus (UVC 15903) P. hectus (UVC 15943) P. sp. "mashpi" (MZUTI 634) P. ocreatus (KU 208508) P. verecundus (MZUTI 539) P. eriphus (QCAZ 32705) P. gentryi (KU 218109) P. cf. curtipes (KU 217871) P. sp. "mashpi" (MZUTI 633) P. sobetes (MZUTI 471) P. sobetes (MZUTI 542) P. truebae (KU 218013) P. sobetes (MZUTI 446) P. mutabilis (MZUTI 2190) P. sobetes (MZUTI 432) P. pyrrhomerus (KU 218030) P. mutabilis (MZUTI 910) P. supernatis (WED 52961) P. sobetes (MZUTI 442) P. jubatus (UVC 15917) P. eriphus (JJM 210) P. mutabilis (MZUTI 909) P. sp. "mashpi" (MZUTI 636) P. surdus (JDL 8862) P. mutabilis (MZUTI 911) P. devillei (JDL 9416 P. vertebralis (KU 177972 P. mutabilis (MZUTI 912) P. cf. thymelensis (QCAZ 16428) P. jubatus (UVC 15911) P. celator (KU 177684) P. thymalopsoides (KU 177861) P. thymelensis (KU 202519) P. verecundus (MZUTI 540) P. leoni (KU 218227) P. verecundus (MZUTI 2114) P. verecundus (MZUTI 541) P. hectus (UVC 15942) P. jubatus (UVC 15920) P. quinquagesimus (KU 179374) P. sobetes (MZUTI 439) P. mutabilis (MZUTI 2191) P. duellmani (WED 53050) P. sobetes (MZUTI 558) P. devillei (KU 2179...