The thrombolytic action of a proteolytic fraction (P1G10) from Carica candamarcensis

Bilheiro, Rogério P.; Braga, Ariadne Duarte; Filho, Marcelo Limborço; Carvalho‐Tavares, Juliana; Agero, Ubirajara; Carvalho, Maria das Graças; Sanchez, Eládio F.; Salas, Carlos; Lopes, Míriam Teresa Paz

doi:10.1016/j.thromres.2013.01.028

Cited by 14 publications

(6 citation statements)

References 44 publications

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“…These two sources of evidence may encourage further experiments on papaya leaf extract as a possible weapon against the COVID-19 pandemic. The protease enzymes and flavonoid contents of other nutrients were also studied in relation to their antioxidant activities [138], as well as their role in T helper-type upregulation and as thrombolytic agents [139], as these biochemical reactions were identified as occurring during COVID-19 pathogenesis, as pointed out in a recent review [140].…”

Section: Perspectivementioning

confidence: 99%

The Future of Carica papaya Leaf Extract as an Herbal Medicine Product

et al. 2021

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Carica papaya (papaya) leaf extract has been used for a long time in a traditional medicine to treat fever in some infectious diseases such as dengue, malaria, and chikungunya. The development of science and technology has subsequently made it possible to provide evidence that this plant is not only beneficial as an informal medication, but also that it has scientifically proven pharmacological and toxicological activities, which have led to its formal usage in professional health care systems. The development of formulations for use in nutraceuticals and cosmeceuticals has caused this product to be more valuable nowadays. The use of good manufacturing practice (GMP) standards, along with the ease of registering this product facilitated by policies of the national government, will absolutely increase the value of papaya leaf extract as a vital nutraceutical and cosmeceutical products in the near future. In this article, we review the potential of papaya leaf extract to be a high-value commodity in terms of its health effects as well as its industrial benefits.

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Section: Perspectivementioning

confidence: 99%

The Future of Carica papaya Leaf Extract as an Herbal Medicine Product

et al. 2021

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“…The ellipsoid fruit, yellow when ripe, contains a thin, aqueous and aromatic pulp, which is consumed cooked to quench the presence of residual proteolytic enzymes [ 1 ]. We showed that a cysteine proteinase enriched fraction from V. cundinamarcensis , named as P1G10, displays a variety of pharmacological activities, such as fibrinogenolytic, fibrinolytic and antithrombotic activities [ 2 ], and tissue repairer effect in cutaneous [ 3 , 4 , 5 ], and gastric lesions [ 6 , 7 ]. Preclinical studies show that P1G10 is non-toxic when used topically [ 4 ] or systemically, with no evidence of mutagenic or genotoxic effects [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Cysteine Proteases from V. cundinamarcensis (C. candamarcensis) Inhibit Melanoma Metastasis and Modulate Expression of Proteins Related to Proliferation, Migration and Differentiation

Lemos

Dittz

Santos

et al. 2018

IJMS

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Previous studies showed that P1G10, a proteolytic fraction from Vasconcellea cundinamarcensis latex, reduced the tumor mass in animals bearing melanoma, increased in vitro DNA fragmentation and decreased cell adhesion. Here, we present some molecular and cellular events related to the antimetastatic effect induced by the CMS-2 fraction derived from P1G10 in metastatic melanoma B16-F10 and melanocyte Melan-a. Using difference gel electrophoresis and mass spectrometry, we identified four proteins overexpressed in tumor cells, all of them related to proliferation, survival, migration and cell invasion, that had their expression normalized upon treatment with CMS-2: nucleophosmin 1, heat shock protein 65, calcyclin binding protein and eukaryotic translation initiation factor 4H. In addition, some antioxidant and glycolytic enzymes show increased expression after exposure to CMS-2, along with an induction of melanogenesis (differentiation marker). The down regulation of cofilin 1, a protein involved in cell motility, may explain the inhibition of cell migration and dendritic-like outgrowth in B16-F10 and Melan-a, observed after CMS-2 treatment. Taken together, it is argued that CMS-2 modulates the expression of proteins related to metastatic development, driving the cell to a more differentiated-like state. These effects support the CMS-2 antimetastatic activity and place this fraction in the category of anticancer agent.

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“…The fraction induces skin epithelization after wound healing caused by dermoabrasions [ 6 ], burns [ 7 ], and gastric lesions [ 8 ]. P1G10 also displays fibrinogenolytic, fibrinolytic and antithrombotic activities, in a dose-dependent manner [ 9 ]. In most situations the biological activity of plant cysteine proteinases is explained by the hydrolytic activity, immunomodulation, and/or binding to circulating anti-proteases, upsetting the homeostasis of endogenous circulating proteinases [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antiangiogenesis, Loss of Cell Adhesion and Apoptosis Are Involved in the Antitumoral Activity of Proteases from V. cundinamarcensis (C. candamarcensis) in Murine Melanoma B16F1

Dittz

Figueiredo

Lemos

et al. 2015

IJMS

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The proteolytic enzymes from V. cundinamarcensis latex, (P1G10), display healing activity in animal models following various types of lesions. P1G10 or the purified isoforms act as mitogens on fibroblast and epithelial cells by stimulating angiogenesis and wound healing in gastric and cutaneous ulcers models. Based on evidence that plant proteinases act as antitumorals, we verified this effect on a murine melanoma model. The antitumoral effect analyzed mice survival and tumor development after subcutaneous administration of P1G10 into C57BL/6J mice bearing B16F1 low metastatic melanoma. Possible factors involved in the antitumoral action were assessed, i.e., cytotoxicity, cell adhesion and apoptosis in vitro, haemoglobin (Hb), vascular endothelial growth factor (VEGF), tumor growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α) content and N-acetyl-glucosaminidase (NAG) activity. We observed that P1G10 inhibited angiogenesis measured by the decline of Hb and VEGF within the tumor, and TGF-β displayed a non-significant increase and TNF-α showed a minor non-significant reduction. On the other hand, there was an increase in NAG activity. In treated B16F1 cells, apoptosis was induced along with decreased cell binding to extracellular matrix components (ECM) and anchorage, without impairing viability.

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The thrombolytic action of a proteolytic fraction (P1G10) from Carica candamarcensis

Cited by 14 publications

References 44 publications

The Future of Carica papaya Leaf Extract as an Herbal Medicine Product

The Future of Carica papaya Leaf Extract as an Herbal Medicine Product

Cysteine Proteases from V. cundinamarcensis (C. candamarcensis) Inhibit Melanoma Metastasis and Modulate Expression of Proteins Related to Proliferation, Migration and Differentiation

Antiangiogenesis, Loss of Cell Adhesion and Apoptosis Are Involved in the Antitumoral Activity of Proteases from V. cundinamarcensis (C. candamarcensis) in Murine Melanoma B16F1

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