The tobacco extensin gene Ext 1.4 is expressed in cells submitted to mechanical constraints and in cells proliferating under hormone control

Hirsinger, Cathy; Salva, Isabelle; Marbach, J.; Durr, Andrée; Fleck, Jacqueline; Jamet, Élisabeth

doi:10.1093/jxb/50.332.343

Cited by 16 publications

(33 citation statements)

References 8 publications

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“…Similar findings had also been reported by Shirsat et al (1996) who detected expression of the extA::GUS fusion gene in the internal and external phloem and in the cortical parenchyma in transgenic tobacco at the regions where the auxiliary stalk was developed. In another study, GUS staining localised expression of the tobacco Ext1.4::GUS fusion gene to the internal and external phloem at stem nodes and also in the floral pedicels (Hirsinger et al, 1999;Salvá and Jamet, 2001). Although these studies show that activation of extensin promoters is limited mainly to the vascular tissue of the stem nodal areas, they do not provide adequate information on either the exact location or the age of the tissues analysed.…”

Section: Discussionmentioning

confidence: 97%

“…Expression of the atExt1 gene was detected in the pollen grains of transgenic tobacco plants carrying the atExt1::GUS fusion gene (most but not all pollen grains were stained blue). To our knowledge, the only study where an extensin gene was reported to be expressed in the pollen grains was the Ext1.4 tobacco extensin gene in tobacco plants harbouring the Ext1.4::GUS fusion gene (Hirsinger et al, 1999). Expression of this transgene was detected in pollen grains before and after pollen grain germination, leading to the suggestion that the Ext1.4 gene was involved in pollen maturation and pollen tube formation (Hirsinger et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…The npExt::GUS and Ext1.4::GUS fusion genes, for example, were transformed into tobacco plants in order to study the expression patterns of the npExt (Tire et al, 1994) and the Ext1.4 (Hirsinger et al, 1999) tobacco extensin genes in a homologous system. Tobacco plants have also been used as a heterologous system to study the expression pattern of extensin genes isolated from species for which an efficient transformation method is lacking.…”

Section: Introductionmentioning

confidence: 99%

“…This detailed analysis consisted of observing successive sections of the transgenic plants, practising our belief that expression analysis should be performed in a very thorough manner in order to detect potential differences among the various tissues. In addition, our study enables the direct comparison of the expression patterns of the various extensin gene family members in tobacco, a widely used experimental system to analyse the developmental and stress-induced expression of many extensin genes in the past (Tire et al, 1994;Wycoff et al, 1995;Ahn et al, 1996;Shirsat et al, 1991Shirsat et al, , 1996Hirsinger et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Merkouropoulos

Shirsat

2012

Genet. Mol. Res.

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ABSTRACT. Histochemical analysis of transgenic tobacco plants harboring the promoter of the Arabidopsis extensin gene atExt1 fused to the β-glucuronidase gene coding sequence demonstrated expression of the transgene in stem tissues. The transgene was expressed in cells of the internal and external phloem at the nodal and internodal regions of the older parts of mature stems. In younger parts of the same stem, expression of the transgene was slightly modified: expression was detected in the internal phloem in the internodal areas. In the nodal areas, the phloem tissue and the surrounding parenchyma were stained, demonstrating transgene expression. Expression was also seen in the phloem of the inflorescence; it was non-specific at the junctions of the flowers to the inflorescence. β-glucuronidase (reporter gene) staining was also observed in the pollen grains and at the base of the corolla.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Merkouropoulos

Shirsat

2012

Genet. Mol. Res.

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“…Histochemical analyses were performed on plantlets from primary transformants propagated in vitro. For fluorometric assays, normalization of samples was performed by protein quantification (Hirsinger et al, 1999). Relative GUS activities were calculated and expressed as pmol MUG per minute per milligram protein.…”

Section: Gus Activity Assaysmentioning

confidence: 99%

Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco

Cheng

Yuan

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The regulative sequence (2273 bp) of the chalcone synthase gene promoter of biloba was cloned by genomic walking. A 2273-bp promoter 5' upstream translation start site of GbCHS was cloned and designated as GbCHSP. pBI121+CHSP:GUS and pBI121-35S:GUS were constructed and transformed into tobacco by LBA4404. We found that GbCHSP could drive transient expression of GUS in tobacco and differentially expressed in root, stem and leaf tissues of this plant. GUS activity regulated by the CHSP promoter were located in tissues (apical meristems) at the growing points of roots and stems. pBI121+CHSP:GUS could be induced by wounding, copper, UV-B, abscisic acid, and ethephon treatments of transgenic seedlings. This activity was weakly Ginkgo biloba chalcone synthase gene promoter in tobacco inhibited by gibberellin. Deletion analysis of the CHSP promoter in transgenic tobacco showed that CHSP1 complete promoter conferred a GUS expression and activity similar to that of 35 S(CaMV). GUS activity dropped dramatically when there were CHSP4, CHSP5 constructs and was almost totally absent when the CHSP6 construct was present. We conclude that the upstream sequence -1548 to -306 of GbCHSP is the main region for transcriptional regulation of the CHS gene and that it is activated by hormone and stress factors in G. biloba. These results will help us to understand the transcriptional regulatory mechanisms involved in GbCHS expression and flavonoid accumulation in G. biloba.

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microRNAs and Mechanical Stress

2011

Mechanical Integration of Plant Cells and Plants

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Mechanical stress, one of the major abiotic stresses, significantly affects plant growth, survival, and reproduction. Recent studies showed that cytoplasmic calcium, reactive oxygen species, and phytohormones play important roles in mechanosensing and subsequent anatomical and morphological changes in plants exposed to mechanical stress. A large number of mechanical stress-responsive genes, particularly those involved in reaction wood formation in tree species, have been isolated and characterized. Importantly, a group of mechanical stressresponsive miRNAs was identified in Populus trichocarpa plants. They were predicted or experimentally validated to regulate genes encoding transcription factors, metabolism and cellular process-related proteins, and many functionunknown proteins. Analyses of miRNA expression patterns and target gene functions indicate that miRNAs play crucial regulatory roles in reaction wood formation. Recent results provide novel and useful information for fully elucidating the genetic and molecular mechanisms of plant responses to mechanical stress.

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The tobacco extensin gene Ext 1.4 is expressed in cells submitted to mechanical constraints and in cells proliferating under hormone control

Cited by 16 publications

References 8 publications

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco

microRNAs and Mechanical Stress

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