The toxic Doppelganger: on the ionic and molecular mimicry of cadmium.

Chmielowska-Bąk, Jagna; Izbiańska, Karolina; Deckert, Joanna

doi:10.18388/abp.2013_1994

Cited by 31 publications

(25 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that, in contrast to calmodulin where manganese ions stabilize the 'closed' state (Senguen and Grabarek, 2012), FhCaBP2 undergoes similar conformational changes with both manganese and calcium ions. Indeed inappropriate cadmiumbinding can lead to toxicity (Chmielowska-Bak et al 2013;Choong et al 2014). However, to date, only limited functional information is available for these proteins and it is not possible to say whether or not manganese-binding is physiologically relevant.…”

Section: Discussionmentioning

confidence: 99%

FhCaBP2: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

Thomas

Timson

2015

Parasitology

View full text Add to dashboard Cite

FhCaBP2 is a Fasciola hepatica protein which belongs to a family of helminth calcium-binding proteins which combine an N-terminal domain containing two EF-hand motifs and a C-terminal dynein light chain-like (DLC-like) domain. Its predicted structure showed two globular domains joined by a flexible linker. Recombinant FhCaBP2 interacted reversibly with calcium and manganese ions, but not with magnesium, barium, strontium, copper (II), colbalt (II), iron (II), nickel, lead or potassium ions. Cadmium (II) ions appeared to bind non-site-specifically and destabilize the protein. Interaction with either calcium or magnesium ions results in a conformational change in which the protein's surface becomes more hydrophobic. The EF-hand domain alone was able to interact with calcium and manganese ions; the DLC-like domain was not. Alteration of a residue (Asp-58 to Ala) in the second EF-hand motif in this domain abolished ion-binding activity. This suggests that the second EF-hand is the one responsible for ion-binding. FhCaBP2 homodimerizes and the extent of dimerization was not affected by calcium ions or by the aspartate to alanine substitution in the second EF-hand. The isolated EF-hand and DLC-like domains are both capable of homodimerization. FhCaBP2 interacted with the calmodulin antagonists trifluoperazine, chlorpromazine, thiamylal and W7. Interestingly, while chlorpromazine and thiamylal interacted with the EF-hand domain (as expected), trifluoperazine and W7 bound to the DLC-like domain. Overall, FhCaBP2 has distinct biochemical properties compared with other members of this protein family from Fasciola hepatica, a fact which supports the hypothesis that these proteins have different physiological roles.

show abstract

Section: Discussionmentioning

confidence: 99%

FhCaBP2: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

Thomas

Timson

2015

Parasitology

View full text Add to dashboard Cite

show abstract

“…On the other hand, their calcium carbonate stores must remain disposable to rapid mobilization upon metabolic demand [ 77 ]. This would probably also lead to a rapid liberation of the trapped Cd 2+ ions which, because of their similar ionic radii, may be confused with Ca 2+ ions by cellular transport mechanisms [ 78 ] and Ca-dependent signalling pathways [ 68 ]. Hence, the inactivation of Cd 2+ on behalf of calcium may be one of the important protective roles exerted by MT in the midgut gland tissue of Littorina littorea .…”

Section: Discussionmentioning

confidence: 99%

Successive Onset of Molecular, Cellular and Tissue-Specific Responses in Midgut Gland of Littorina littorea Exposed to Sub-Lethal Cadmium Concentrations

Benito

Niederwanger

Izagirre

et al. 2017

IJMS

View full text Add to dashboard Cite

Cadmium (Cd) is one of the most harmful metals, being toxic to most animal species, including marine invertebrates. Among marine gastropods, the periwinkle (Littorina littorea) in particular can accumulate high amounts of Cd in its midgut gland. In this organ, the metal can elicit extensive cytological and tissue-specific alterations that may reach, depending on the intensity of Cd exposure, from reversible lesions to pathological cellular disruptions. At the same time, Littorina littorea expresses a Cd-specific metallothionein (MT) that, due to its molecular features, expectedly exerts a protective function against the adverse intracellular effects of this metal. The aim of the present study was, therefore, to assess the time course of MT induction in the periwinkle’s midgut gland on the one hand, and cellular and tissue-specific alterations in the digestive organ complex (midgut gland and digestive tract) on the other, upon exposure to sub-lethal Cd concentrations (0.25 and 1 mg Cd/L) over 21 days. Depending on the Cd concentrations applied, the beginning of alterations of the assessed parameters followed distinct concentration-dependent and time-dependent patterns, where the timeframe for the onset of the different response reactions became narrower at higher Cd concentrations compared to lower exposure concentrations.

show abstract

“…The hyper-accumulation and toxicity of heavy metals is now a worldwide problem leading to agriculture losses and hazardous health effects as metals enter the food chain (Chmielowska-Bak et al 2013;Sigel et al 2013). Among heavy metals, cadmium (Cd)is a toxic pollutant which enters the environment from many industrial processes; electroplating, production of nickel-cadmium batteries, plastic, pigments, ceramics and cement manufacturing (Nriagu, 1996).…”

Section: Biometric and Biochemical Attributes Of Alfalfa Seedlings Asmentioning

confidence: 99%

Biometric and biochemical attributes of alfalfa seedlings as indicators of stress induced by excessive cadmium

Mahmood

Malik

Tabassum

et al. 2014

J. Soil Sci. Plant Nutr.

View full text Add to dashboard Cite

The biometric and biochemical attributes of alfalfa (Medicago sativa L.) seedlings were studied after their exposure to 0-120 μM cadmium for 28 days using hydroponic culture. The growth, photosynthetic area and pigment contents (chlorophyll a, b and total) declined significantly (p ≤ 0.05) in the presence of high cadmium concentrations (90 and 120 μM). A steady increase in lipid peroxidation assessed via MDA production was observed with increasing levels of cadmium. Cadmium uptake by the plant tissues was concentration dependent. The roots accumulated 1020 µg g-1 of cadmium which was two folds than leaves. An enhanced production up to 600 µg g -1 of proline was observed at higher levels of cadmium. Though, cadmium toxicity was expressed in terms of decline in growth variables, chlorophyll content and oxidative damage but restricted transfer of the metal to the aerial tissue and greater production of proline in response to higher metal content seems to alleviate cadmium toxicity. Thus, ability of alfalfa plants to tolerate high cadmium concentrations can be a manifestation of effective defensive mechanism derived from differential accumulation of metal in plant tissues in addition to enhanced production of proline.

show abstract

The toxic Doppelganger: on the ionic and molecular mimicry of cadmium.

Cited by 31 publications

References 94 publications

FhCaBP2: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

FhCaBP2: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

Successive Onset of Molecular, Cellular and Tissue-Specific Responses in Midgut Gland of Littorina littorea Exposed to Sub-Lethal Cadmium Concentrations

Biometric and biochemical attributes of alfalfa seedlings as indicators of stress induced by excessive cadmium

Contact Info

Product

Resources

About