The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies (Lilium spp.) and regulates pigment quantity

Yamagishi, Masumi; Yoshida, Yusuke; Nakayama, Masayoshi

doi:10.1007/s11032-011-9675-6

Cited by 64 publications

(48 citation statements)

References 40 publications

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“…LhMYB12, PAP1 and AtMYB113 control accumulation of anthocyanins by regulation of expression of biosynthetic genes202122. Expression levels of LhMYB12 are positively correlated with tepal anthocyanin pigmentation in Asiatic hybrid lilies19. Cacao TcMYB113 was recently identified as a likely candidate for regulation of green/red pod colour23.…”

Section: Resultsmentioning

confidence: 99%

The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB

et al. 2014

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Oil palm, a plantation crop of major economic importance in Southeast Asia, is the predominant source of edible oil worldwide. We report the identification of the VIRESCENS (VIR) gene, which controls fruit exocarp colour and is an indicator of ripeness. VIR is a R2R3-MYB transcription factor with homology to Lilium LhMYB12 and similarity to Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1). We identify five independent mutant alleles of VIR in over 400 accessions from sub-Saharan Africa that account for the dominant-negative virescens phenotype. Each mutation results in premature termination of the carboxy-terminal domain of VIR, resembling McClintock’s C1-I allele in maize. The abundance of alleles likely reflects cultural practices, by which fruits were venerated for magical and medicinal properties. The identification of VIR will allow selection of the trait at the seed or early-nursery stage, 3-6 years before fruits are produced, greatly advancing introgression into elite breeding material.

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Section: Resultsmentioning

confidence: 99%

The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB

et al. 2014

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“…‘Strawberry & Cream’ developed splatters on a pink background (Fig. S2) and possessed LhMYB12‐Lat and LhMYB12‐Ren ( LhMYB12‐Ren regulates full‐tepal pigmentation; Yamagishi et al ., ), whereas ‘Connecticut King’ had yellow tepals without spots and did not amplify these sequences (Fig. S5).…”

Section: Resultsmentioning

confidence: 99%

“…S1; Nakatsuka et al ., ; Lai et al ., ), and LhMYB12, LhMYB6 and LhbHLH2 transcription factors coordinately regulate the transcription of the biosynthesis genes (Nakatsuka et al ., ; Yamagishi et al ., ). The action of LhMYB12 gives a full‐pink phenotype, and its transcription levels affect the intensity of anthocyanin colour (Yamagishi et al ., ). LhMYB6 mainly regulates vegetative pigmentation (Yamagishi et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…One of the most attractive features of Asiatic hybrid lilies is their large colour variation. The tepals of Asiatic hybrid lilies are pigmented pink and chocolate brown by anthocyanins, yellow and orange by carotenoids, and red by the combined presence of anthocyanins and orange carotenoids (Deli et al ., ; Nørbæk & Kondo, ; Yamagishi et al ., , ; Jeknić et al ., ). Anthocyanins in lily tepals comprise cyanidin 3‐ O ‐β‐rutinoside and cyanidin 3‐ O ‐β‐rutinoside‐7‐ O ‐β‐glucoside; the former is the major anthocyanin, whereas the latter accumulates in small amounts in only a few cultivars (Nørbæk & Kondo, ).…”

Section: Introductionmentioning

confidence: 99%

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The novel allele of the LhMYB12 gene is involved in splatter‐type spot formation on the flower tepals of Asiatic hybrid lilies (Lilium spp.)

2013

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SummaryMany angiosperm families develop spatially regulated anthocyanin spots on their flowers. The Asiatic hybrid lily (Lilium spp.) cv 'Latvia' develops splatter-type spots on its tepals. The splatters arise simply from the deposition of anthocyanin pigments in the tepal epidermis.To determine how splatter development was regulated, we analysed the transcription of anthocyanin biosynthesis genes, and isolated and characterized an R2R3-MYB gene specific to splatter pigmentation.All anthocyanin biosynthesis genes were expressed in splatter-containing regions of tepals, but not in other regions, indicating that splatter pigmentation is caused by the transcriptional regulation of biosynthesis genes. Previously characterized LhMYB12 regulators were not involved in splatter pigmentation, but, instead, a new allele of the LhMYB12 gene, LhMYB12-Lat, isolated in this study, contributed to splatter development. In 'Latvia' and other lily plants expressing splatters, LhMYB12-Lat was preferentially transcribed in the splatter-containing region of tepals. Progeny segregation analysis showed that LhMYB12-Lat genotype and splatter phenotype were co-segregated among the F 1 population, indicating that LhMYB12-Lat determines the presence or absence of splatters.LhMYB12-Lat contributes to splatter development, but not to full-tepal pigmentation and raised spot pigmentation. As a result of its unique sequences and different transcription profiles, this new allele of LhMYB12 should be a novel R2R3-MYB specifically associating with splatter spot development.

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“…Anthocyanins are one of the major pigments affecting the coloration of the floral tepals in Lilium spp. [1][2][3][4]. Anthocyanins are synthesized in the cytosol but their transfer to the vacuole is necessary for plant tissues to exhibit brilliant colors [5,6].…”

Section: Introductionmentioning

confidence: 99%

Cloning and Functional Characterization of a Flavonoid Transport-Related MATE Gene in Asiatic Hybrid Lilies (Lilium spp.)

Yang

Cao

et al. 2020

Genes

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Previous studies have suggested that multidrug and toxic compound extrusion (MATE) proteins might be involved in flavonoid transportation. However, whether MATE proteins are involved in anthocyanin accumulation in Lilium is unclear. Here, a flavonoid transport-related MATE candidate gene, LhDTX35, was cloned from the Asiatic hybrid lily cultivar ‘Tiny Padhye’ by rapid amplification of 5’ and 3’ cDNA ends (RACE) and found to encode 507 amino acids. BLASTx results indicated that LhDTX35 showed high homology to the DTX35 genes of other species. Bioinformatics analysis predicted that the protein encoded by LhDTX35 possessed 12 typical transmembrane segments and had functional domains typical of the MATE-like superfamily. Phylogenetic analysis grouped LhDTX35 in the same clade as the DTX35 of other species. Notably, the expression pattern of LhDTX35 was positively correlated with floral anthocyanin accumulation in ‘Tiny Padhye’. A subcellular localization assay showed that the protein encoded by LhDTX35 was plasmalemma localized but not nuclear, indicating that the LhDTX35 gene may function as a carrier protein to transport anthocyanins in Lilium. Functional complementation of the Arabidopsis DTX35 gene demonstrated that LhDTX35 could restore silique-infertility and the anthocyaninless phenotype of an Arabidopsis DTX35 mutant. These results indicated that LhDTX35 might be involved in anthocyanin accumulation in Lilium.

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The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies (Lilium spp.) and regulates pigment quantity

Cited by 64 publications

References 40 publications

The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB

The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB

The novel allele of the LhMYB12 gene is involved in splatter‐type spot formation on the flower tepals of Asiatic hybrid lilies (Lilium spp.)

Cloning and Functional Characterization of a Flavonoid Transport-Related MATE Gene in Asiatic Hybrid Lilies (Lilium spp.)

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