The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae

Abstract

Edited by Francesc Posas Keywords:Yeast repressor activator protein Cell wall Slt2p-phosphorylation Cell wall integrity pathway Cell wall thickness Cell wall perturbing agent a b s t r a c t Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N-terminal truncation of Rap1p (Rap1DN) leads to hypersensitivity towards elevated temperature and cell-wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1DN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1DN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis.

A systematic assessment of chemical, genetic, and epigenetic factors influencing the activity of anticancer drug KP1019 (FFC14A)

“…Furthermore to confirm that indeed KP1019 targets CWI pathway, we screened mutants of Rap1, a Repressor/Activator site binding Protein, for KP1019 tolerance ( Supplementary Figure 2B ). Intriguingly, rap1ΔN cells that have defective cell wall [ 36 ] were found to be KP1019 sensitive, which was relieved upon sorbitol supplementation (Figure 2D ). Collectively, our results indicate that KP1019 may affect directly or indirectly the cell wall and intact CWI pathway is required for its tolerance.…”

The multifunctional transcription factor Rap1 a regulator of yeast physiology

“…deletion mutant. Altogether, we have shown the novel function of ScRap1 N-terminal domain in the regulation of yeast cell wall integrity (21).…”

Functional duplication of Rap1 in methylotrophic yeasts

“…In addition to its importance at telomeres, ScRap1 has been explicitly shown to be a central regulator of transcription 14 . Among the first identified and most prominent targets is the set of ribosomal protein gene promoters.…”

“…Near the N-terminus, Rap1 contains a BRCT domain that interacts with Gcr1 factor, and this interaction is necessary for the regulation of glycolytic genes transcription 13 . In addition, Rap1 BRCT has been implicated in the maintenance of cell wall homeostasis 14 . The Rap1 C-terminal (RCT) domain plays a crucial role at S .…”