The transcription factor Sox30 is involved in Nile tilapia spermatogenesis

Wei, Ling; Tang, Yaohao; Zeng, Xianhai; Li, Yueqin; Zhang, Song; Deng, Li; Wang, Lingsong; Wang, Deshou

doi:10.1016/j.jgg.2021.11.003

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…Similarly, only somatic cells but not germ cells were feminized in the XY amhy − ; foxl3 -/- (n = 4) and XX/XY gsdf -/- ; foxl3 -/- (n = 5) double mutants at 120 dah as demonstrated by IF using female Cyp19a1a and 42Sp50, male Cyp11c1, Gsdf and Creb1b markers. Sox30, which is mainly detected in spermatozoa in testis [ 55 ], was detected in the gonads of the amhy − ; foxl3 -/- and gsdf -/- ; foxl3 -/- double mutants at 120 dah ( S8A Fig ). These data suggested that in the presence of dmrt1 , even if amhy or gsdf was mutated, germ cells still entered into spermatogenesis in the absence of foxl3 .…”

Section: Resultsmentioning

confidence: 99%

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Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia

Qi,

Dai,

Zhou

et al. 2024

PLoS Genet

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Sex is determined by multiple factors derived from somatic and germ cells in vertebrates. We have identified amhy, dmrt1, gsdf as male and foxl2, foxl3, cyp19a1a as female sex determination pathway genes in Nile tilapia. However, the relationship among these genes is largely unclear. Here, we found that the gonads of dmrt1;cyp19a1a double mutants developed as ovaries or underdeveloped testes with no germ cells irrespective of their genetic sex. In addition, the gonads of dmrt1;cyp19a1a;cyp19a1b triple mutants still developed as ovaries. The gonads of foxl3;cyp19a1a double mutants developed as testes, while the gonads of dmrt1;cyp19a1a;foxl3 triple mutants eventually developed as ovaries. In contrast, the gonads of amhy;cyp19a1a, gsdf;cyp19a1a, amhy;foxl2, gsdf;foxl2 double and amhy;cyp19a1a;cyp19a1b, gsdf;cyp19a1a;cyp19a1b triple mutants developed as testes with spermatogenesis via up-regulation of dmrt1 in both somatic and germ cells. The gonads of amhy;foxl3 and gsdf;foxl3 double mutants developed as ovaries but with germ cells in spermatogenesis due to up-regulation of dmrt1. Taking the respective ovary and underdeveloped testis of dmrt1;foxl3 and dmrt1;foxl2 double mutants reported previously into consideration, we demonstrated that once dmrt1 mutated, the gonad could not be rescued to functional testis by mutating any female pathway gene. The sex reversal caused by mutation of male pathway genes other than dmrt1, including its upstream amhy and downstream gsdf, could be rescued by mutating female pathway gene. Overall, our data suggested that dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia.

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Section: Resultsmentioning

confidence: 99%

“…For sperm motility analysis, sperm was collected from WT XY and XX foxl3 -/- fish at 120 dah. After a dilution at 1:10 with phosphatebuffered saline, 10 mL diluted semen was added to the counting chamber of the sperm count plate and captured on the stage of a Leica DM500 light microscope (Leica, Germany) [ 55 ].…”

Section: Methodsmentioning

confidence: 99%

Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia

Qi,

Dai,

Zhou

et al. 2024

PLoS Genet

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“…Recently, sox30 is specifically expressed in the testes and plays essential roles in Nile tilapia spermatogenesis. Considering the similarity in sox30 sequence, similar function could occur in the process of C. carpio spermatogenesis ( 37 ). Another male-specific gene ( amh ) was identified in Cluster 4 that reached its peak expression level at stage 3.…”

Section: Resultsmentioning

confidence: 99%

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp

et al. 2022

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The common carp (Cyprinus carpio) accounts for approximately 10% of the annual freshwater aquaculture production and is an ideal model to study cyprinidae reproduction. Female common carp grow faster than the males; therefore, related research presents an opportunity with high application value. Although we have a detailed understanding of common carp’s early gonadal differentiation process, information about genome-wide gene expression, regulation, and underlying molecular mechanisms during this process remain limited. Here, time-course data comprising six key stages during testicular differentiation and maturation were investigated to further understand the molecular mechanisms underlying the testicular development in cyprinid species. After integrating these time-series data sets, common carp genome, including 98,345 novel transcripts and 3,071 novel genes were re-annotated and precisely updated. Gene co-expression network analysis revealed that the ubiquitin-mediated proteolysis pathway was essential for metabolism during testicular differentiation in the endocrine system of C. carpio. Functional enrichment analyses indicated that genes mainly related to amino acid metabolism and steroid hormone synthesis were relatively highly expressed at the testicular undifferentiation stages, whereas genes associated with cell cycle and meiosis were expressed from the beginning of testicular differentiation until maturation. The dynamics of alternative splicing events demonstrated that exon skipping accounted for majority of the alternative splicing events in the testis and the brain during gonad development. Notably, several potential male-specific genes (fanci and sox30) and brain-specific genes (oxt, gad2, and tac1, etc.) were identified. Importantly, we traversed beyond the level of transcription to test for stage- and gonad-specific alternative splicing patterns between the brain and testis. This study is the first to describe a comprehensive landscape of alternative splicing events and gene expression patterns during gonadogenesis in common carp. This work is extremely valuable to elucidate the mechanisms underlying gonadal differentiation in Cyprinidae as well as other fish species.

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“…The sox gene family was also expanded (43 members) in P. lineatus compared with that in C. bouderius and H. wyckioides (26 and 25 members, respectively), which was mainly due to sox30 (Supplementary Figure S6 and Table S15). The sox30 gene is involved in spermatogenesis in fish ( Wei et al, 2022 ), suggesting that P. lineatus may also have adapted to the marine environment during germ cell processes.…”

Section: Dear Editormentioning

confidence: 99%

Genome of striped eel catfish (<i>Plotosus lineatus</i>) provides insights into the adaptive evolution of amphidromous fish

Shao¹,

Wu²,

Xu³

et al. 2023

Zoological Research

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A: Ancestral chromosome construction of Siluriformes. Conserved blocks are color-coded according to their chromosomal source in Siluriformes ancestor. Designated FTSs (distance between adjacent different colored blocks of less than 1 Mb) are identified by letters in enlarged figure. B: Comparison of TE content between selected fusion site and other sites on corresponding chromosome (100 kb window, significant differences: NS: Not significant; ** : P <0.01; *** : P <0.001). Letters on horizontal axis indicate different fusion sites, and different colors indicate selected fusion site and other sites on corresponding chromosome. C: Heatmap of TE superfamily content at selected fusion site. D: Pairwise whole-genome alignments across C. bouderius , H. wyckioides , and P. lineatus genomes. Each horizontal bar represents one chromosome, and chromosome IDs of the three species are labeled.

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The transcription factor Sox30 is involved in Nile tilapia spermatogenesis

Cited by 8 publications

References 44 publications

Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia

Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp

Genome of striped eel catfish (<i>Plotosus lineatus</i>) provides insights into the adaptive evolution of amphidromous fish

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