The transcriptional characteristics of mast cells derived from skin tissue in type 2 diabetes patients at the single-cell level

Liao, Bingye; Ouyang, Qiuyi; Song, Hongqin; Wang, Ziqi; Ou, Jinhua; Huang, Jinxin; Liu, Liang

doi:10.1016/j.acthis.2021.151789

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…The significant elevation in these MC markers in diabetic conditions emphasizes the potential role of MCs in the inflammatory milieu of the IPFP. Recent single-cell analyses have revealed altered gene expression profiles in MCs derived from individuals with DM, specifically the upregulation of PAXIP1 and ARG1 [41]. In the HbA1c ≥ 6.5 group, PAXIP1 and ARG1 expression were significantly higher in MC-RF than MC-PF.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolic conditions induce alterations in MC phenotype and function [40][41][42][43]. In diabetic animals, there was an elevated presence of MCs in the kidneys, and these cells were identified to release diverse mediators, including chymase, tryptase, and cathepsin Gs [40].…”

Section: Discussionmentioning

confidence: 99%

“…Recent single-cell analysis has unveiled an elevated expression of ARG1, IL3RA, PAXIP1, and HAS1 in MC derived from the skin of individuals with diabetes [41]. To delineate the characteristics of MC derived from diabetic KOA patients, we utilized magnetic bead methods to isolate both the MC-RF and MC-PF.…”

Section: Isolation Of MC Using Magnetic Beadsmentioning

confidence: 99%

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Association between High HbA1c Levels and Mast Cell Phenotype in the Infrapatellar Fat Pad of Patients with Knee Osteoarthritis

Tsukada,

Takata,

Aikawa

et al. 2024

IJMS

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Diabetes mellitus (DM) has been suggested as a potential risk factor for knee osteoarthritis (KOA), and its underlying mechanisms remain unclear. The infrapatellar fat pad (IPFP) contributes to OA through inflammatory mediator secretion. Mast cells’ (MCs) role in diabetic IPFP pathology is unclear. In 156 KOA patients, hemoglobin A1c (HbA1c) was stratified (HbA1c ≥ 6.5, n = 28; HbA1c < 6.5, n = 128). MC markers (TPSB2, CPA3) in IPFP were studied. Propensity-matched cohorts (n = 27 each) addressed demographic differences. MC-rich fraction (MC-RF) and MC-poor fraction (MC-PF) were isolated, comparing MC markers and genes elevated in diabetic skin-derived MC (PAXIP1, ARG1, HAS1, IL3RA). TPSB2 and CPA3 expression were significantly higher in HbA1c ≥ 6.5 vs. <6.5, both before and after matching. MC-RF showed higher TPSB2 and CPA3 expression than MC-PF in both groups. In the HbA1c ≥ 6.5 group, PAXIP1 and ARG1 expression were significantly higher in the MC-RF than MC-PF. However, no statistical difference in the evaluated genes was detected between the High and Normal groups in the MC-RF. Elevated TPSB2 and CPA3 levels in the IPFP of high HbA1c patients likely reflect higher numbers of MCs in the IPFP, though no difference was found in MC-specific markers on a cell-to-cell basis, as shown in the MC-RF comparison. These findings deepen our understanding of the intricate interplay between diabetes and KOA, guiding targeted therapeutic interventions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Association between High HbA1c Levels and Mast Cell Phenotype in the Infrapatellar Fat Pad of Patients with Knee Osteoarthritis

Tsukada,

Takata,

Aikawa

et al. 2024

IJMS

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“…In some cases, however, too many mast cells can hinder wound healing. A study on mast cells in the skin of patients with type 2 diabetes showed that high expression of IL-3R mast cell clusters may be associated with chronic skin inflammation ( 34 ). McS-01, an indole-carboxyamide-type mast cell stabilizer developed by Tellechea et al., was shown to polarize macrophage phenotypes to accelerate wound healing in diabetic mice ( 35 ).…”

Section: Role Of Inflammatory Cells In the Inflammatory Microenvironmentmentioning

confidence: 99%

Inflammatory Microenvironment of Skin Wounds

et al. 2022

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Wound healing is a dynamic and highly regulated process that can be separated into three overlapping and interdependent phases: inflammation, proliferation, and remodelling. This review focuses on the inflammation stage, as it is the key stage of wound healing and plays a vital role in the local immune response and determines the progression of wound healing. Inflammatory cells, the main effector cells of the inflammatory response, have been widely studied, but little attention has been paid to the immunomodulatory effects of wound healing in non-inflammatory cells and the extracellular matrix. In this review, we attempt to deepen our understanding of the wound-healing microenvironment in the inflammatory stage by focusing on the interactions between cells and the extracellular matrix, as well as their role in regulating the immune response during the inflammatory stage. We hope our findings will provide new ideas for promoting tissue regeneration through immune regulation.

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The evolving role of mast cells in wound healing: insights from recent research and diverse models

Guth,

Limjunyawong,

Pundir

2024

Immunol Cell Biol

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Chronic wounds significantly burden health care systems worldwide, requiring novel strategies to ease their impact. Many physiological processes underlying wound healing are well studied but the role of mast cells remains controversial. Mast cells are innate immune cells and play an essential role in barrier function by inducing inflammation to defend the host against chemical irritants and infections, among others. Many mast cell–derived mediators have proposed roles in wound healing; however, in vivo evidence using mouse models has produced conflicting results. Recently, studies involving more complex wound models such as infected wounds, diabetic wounds and wounds healing under psychological stress suggest that mast cells play critical roles in these processes. This review briefly summarizes the existing literature regarding mast cells in normal wounds and the potential reasons for the contradictory results. Focus will be placed on examining more recent work emerging in the last 5 years that explores mast cells in more complex systems of wound healing, including infection, psychological stress and diabetes, with a discussion of how these discoveries may inspire future work in the field.

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The transcriptional characteristics of mast cells derived from skin tissue in type 2 diabetes patients at the single-cell level

Cited by 5 publications

References 21 publications

Association between High HbA1c Levels and Mast Cell Phenotype in the Infrapatellar Fat Pad of Patients with Knee Osteoarthritis

Association between High HbA1c Levels and Mast Cell Phenotype in the Infrapatellar Fat Pad of Patients with Knee Osteoarthritis

Inflammatory Microenvironment of Skin Wounds

The evolving role of mast cells in wound healing: insights from recent research and diverse models

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