The Transcriptional Repressor Activator Protein Rap1p Is a Direct Regulator of TATA-binding Protein

Bendjennat, Mourad; Weil, P A

doi:10.1074/jbc.m709436200

Cited by 12 publications

(13 citation statements)

References 96 publications

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“…Thus, we preferentially focused on the transcription factor Rap1p as a target of Gcn4p, as many Rap1p target genes have been shown to be repressed by 3‐AT in a Gcn4p‐dependent manner (Natarajan et al , 2001). In addition, many recent studies had revealed that several transcription factors bind to Rap1p directly, and this interaction was important for initiation mechanism for full transcription of RP genes (Garbett et al , 2007; Rudra et al , 2007; Bendjennat and Weil, 2008; Papai et al , 2010). Therefore, physical interaction between Rap1p and Gcn4p was strongly expected.…”

Section: Resultsmentioning

confidence: 99%

“…These results indicated that 20% of RP mRNA appears to be decreased by other mechanisms not yet elucidated. Moreover, the binding domain of Rap1p, which is responsible for Gcn4p binding, is exactly identical to that for TBP binding (Bendjennat and Weil, 2008). These led us to hypothesize that Gcn4p could hinder the association of Rap1p with general transcription machineries, such as TFIID.…”

Section: Discussionmentioning

confidence: 99%

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Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation

et al. 2010

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Gcn4p is a well-characterized bZIP transcription factor that activates more than 500 genes encoding amino acids and purine biosynthesis enzymes, and many stressresponse genes under various stress conditions. Under these stresses, it had been shown that transcriptions of ribosomal protein (RP) genes were decreased. However, the detailed mechanism of this downregulation has not been elucidated. In this study, we present a novel mechanistic model for a repressive role of Gcn4p on RP transcription, especially under amino-acid starvation. It was found that Gcn4p bound directly to Rap1p, which in turn inhibited Esa1p-Rap1p binding. The inhibition of Esa1p recruitment to RP promoters ultimately reduced the level of histone H4 acetylation and RP transcription. These data revealed that Gcn4p has simultaneous dual roles as a repressor for RP genes as well as an activator for amino-acid biosynthesis genes. Moreover, our results showed evidence of a novel link between general control of amino-acid biosynthesis and ribosome biogenesis mediated by Gcn4p at an early stage of adaptation to amino-acid starvation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation

et al. 2010

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“…However, our anchoring experiments indicate that TBP plays a role in +1 positioning at only a limited set of genes dominated by those encoding ribosomal proteins. We speculate that this may be linked to the observation that Rap1, which serves as the PTF at many of these genes, can directly interact with both TBP and several TAF proteins in TFIID (Bendjennat and Weil, 2008;Garbett et al, 2007). It is also possible that the +1 nucleosome simply moves to a thermodynamically favorable position determined by local DNA sequence features following removal of both RSC and an upstream PTF.…”

Section: Additional Factors Determining +1 Nucleosome Positionmentioning

confidence: 98%

Sequence-directed action of RSC remodeler and pioneer factors positions +1 nucleosome to facilitate transcription

Kubik¹,

O’Duibhir

et al. 2018

Preprint

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Accessible chromatin is important for RNA polymerase II recruitment and transcription initiation at eukaryotic promoters. We investigated the mechanistic links between promoter DNA sequence, nucleosome positioning and transcription. Our results indicate that precise positioning of the transcription start site-associated +1 nucleosome in yeast is critical for efficient TBP binding, and is driven by two key factors, the essential chromatin remodeler RSC and a small set of ubiquitous pioneer transcription factors. We find no evidence for recruitment of RSC by pioneer factors, but show instead that the strength and directionality of RSC action on nucleosomes depends upon the arrangement of two specific DNA motifs that promote its binding and nucleosome displacement activity at promoters. Thus, despite their widespread co-localization, RSC and pioneer factors predominantly act independently to generate accessible chromatin. Our results provide insight into how promoter DNA sequence instructs trans-acting factors to control nucleosome architecture and stimulate transcription initiation.(149 words)

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“…However, our anchoring experiments indicate that TBP plays a role in +1 positioning at only a limited set of genes dominated by those encoding ribosomal proteins. We speculate that this may be linked to the observation that Rap1, which serves as the GRF at many of these genes, can directly interact with both TBP and several TBP-associated factor (TAF) proteins in TFIID (Bendjennat and Weil, 2008;Garbett et al, 2007). It is also possible that the +1 nucleosome simply moves to a thermodynamically favorable position determined by local DNA sequence features following removal of both RSC and an upstream GRF.…”

Section: Additional Factors Determining +1 Nucleosome Positionmentioning

confidence: 98%

Sequence-Directed Action of RSC Remodeler and General Regulatory Factors Modulates +1 Nucleosome Position to Facilitate Transcription

et al. 2018

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Accessible chromatin is important for RNA polymerase II recruitment and transcription initiation at eukaryotic promoters. We investigated the mechanistic links between promoter DNA sequence, nucleosome positioning, and transcription. Our results indicate that positioning of the transcription start site-associated +1 nucleosome in yeast is critical for efficient TBP binding and is driven by two key factors, the essential chromatin remodeler RSC and a small set of ubiquitous general regulatory factors (GRFs). Our findings indicate that the strength and directionality of RSC action on promoter nucleosomes depends on the arrangement and proximity of two specific DNA motifs. This, together with the effect on nucleosome position observed in double depletion experiments, suggests that, despite their widespread co-localization, RSC and GRFs predominantly act through independent signals to generate accessible chromatin. Our results provide mechanistic insight into how the promoter DNA sequence instructs trans-acting factors to control nucleosome architecture and stimulate transcription initiation.

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The Transcriptional Repressor Activator Protein Rap1p Is a Direct Regulator of TATA-binding Protein

Cited by 12 publications

References 96 publications

Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation

Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation

Sequence-directed action of RSC remodeler and pioneer factors positions +1 nucleosome to facilitate transcription

Sequence-Directed Action of RSC Remodeler and General Regulatory Factors Modulates +1 Nucleosome Position to Facilitate Transcription

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