The Transport Form of Free Fatty Acids in Rat Serum A study in vitro using gel filtration

Abstract

Goransson, G . The transport form of.fiee fat& acids in rat serum. A stu& in vitro using gelJLtration. Acta physiol. scand. 1965. 64. 269-274. -The partition of labeled fatty acids between the protein and water phases in rat serum has been studied in vitro using gel filtration on Sephadex G-200 columns. Lauric, capric and caproic acids were to an increasing degree distributed over the total volume of the column, whereas all saturated fatty acids as well as unsaturated acids with a chain length of 14 carbon atoms or more were completely eluted in combination with protein. The saturated acids were, with increasing number of carbon atoms, to an increasing extent eluted with the lipoprotein fraction. More than 98 yo of all the unsaturated fatty acids studied were eluted with the albumin peak. When the amount of oleic acid was increased above a mole ratio of total fatty acid to albumin of 3-6, the percentage of radioactivity recovered with the albumin fraction decreased.