The Treatment of Complementary and Alternative Medicine on Premature Ovarian Failure

Lin, Jing; Wu, Danping; Jia, Liyan; Liang, Mengmeng; Liu, Siyu; Qin, Zhen; Zhang, Jiao; Han, Yufen; Liu, Songjiang; Zhang, Yuehui

doi:10.1155/2021/6677767

Cited by 22 publications

(7 citation statements)

References 59 publications

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“…For instance, TGF-β1 induces TEX14-IT1 transcription in a SMAD4-dependent manner, and TEX14-IT1 mediates the antiapoptotic and pro-proliferative effects of TGF-β1 in GCs [ 31 ]. SMAD4 is a key regulatory factor in the production of FSH [ 32 , 33 ]. Bone morphogenetic protein 15 (BMP15) regulates FSH receptor (FSHR) through TGF-β receptor II and SMAD4 signaling in porcine GCs to regulate follicular development [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4

Ju,

Zhao,

et al. 2024

Reprod Biol Endocrinol

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Background In our previous investigation, we revealed a significant increase in the expression of microRNA-6881-3p (miR-6881-3p) in follicular fluid granulosa cells (GCs) from women with diminished ovarian reserve (DOR) compared to those with normal ovarian reserve (NOR). However, the role of miR-6881-3p in the development of DOR remains poorly understood. Objective This study aimed to elucidate the involvement of miR-6881-3p in the regulation of granulosa cells (GCs) function and the pathogenesis of DOR. Materials and methods Initially, we assessed the expression levels of miR-6881-3p in GCs obtained from human follicular fluid in both NOR and DOR cases and explored the correlation between miR-6881-3p expression and clinical outcomes in assisted reproduction technology (ART). Bioinformatic predictions and dual-luciferase reporter assays were employed to identify the target gene of miR-6881-3p. Manipulation of miR-6881-3p expression was achieved through the transfection of KGN cells with miR-6881-3p mimics, inhibitor, and miRNA negative control (NC). Following transfection, we assessed granulosa cell apoptosis and cell cycle progression via flow cytometry and quantified target gene expression through quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB) analysis. Finally, we examined the correlation between target gene expression levels in GCs from NOR and DOR patients and their association with ART outcomes. Results Our findings revealed elevated miR-6881-3p levels in GCs from DOR patients, which negatively correlated with ovarian reserve function and ART outcomes. We identified a direct binding interaction between miR-6881-3p and the 3’-untranslated region of the SMAD4. Transfection with miR-6881-3p mimics induced apoptosis in KGN cell. Furthermore, miR-6881-3p expression negatively correlated with both mRNA and protein levels of the SMAD4. The mRNA and protein levels of SMAD4 were notably reduced in GCs from DOR patients, and SMAD4 mRNA expression positively correlated with ART outcomes. In addition, the mRNA levels of FSHR, CYP11A1 were notably reduced after transfection with miR-6881-3p mimics in KGN cell, while LHCGR notably increased. The mRNA and protein levels of FSHR, CYP11A1 were notably reduced in GCs from DOR patients, while LHCGR notably increased. Conclusion This study underscores the role of miR-6881-3p in directly targeting SMAD4 mRNA, subsequently diminishing granulosa cell viability and promoting apoptosis, and may affect steroid hormone regulation and gonadotropin signal reception in GCs. These findings contribute to our understanding of the pathogenesis of DOR.

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Section: Discussionmentioning

confidence: 99%

miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4

Ju,

Zhao,

et al. 2024

Reprod Biol Endocrinol

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“…The problem can be further complicated by inaccurate quotations included in literature reviews; an example is the quotation of this protocol on auricular acupuncture for premature ovarian insufficiency [ 35 ]: “Luo et al (...) conducted a systematic review and meta-analysis of the efficacy and safety of ear acupuncture in the treatment of POI by searching multiple databases before August 2020. The results showed that stimulation of specific ear points can enhance the physiological functions of specific parts of the human body, promote qi and blood circulation, and regulate the reproductive function of the hypothalamus-pituitary-ovarian axis” [ 36 ]. This is particularly disturbing given the specific nature of the information derived from a protocol in which the authors state: “We expect to provide reference for premature ovarian insufficiency treatment in the field of traditional Chinese medicine”.…”

Section: Discussionmentioning

confidence: 99%

Quotation Accuracy of Systematic Review and Meta-Analysis Protocols on Acupuncture

et al. 2021

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Currently, published systematic review protocols (SR protocols) have increasingly become a new trend in fields such as acupuncture and are therefore a new source of quotations in these fields. Systematic reviews are considered the pinnacle of the evidence pyramid as they embody comprehensive literature searching. Quotations are key elements to achieve this goal as they can support the assertions of the original authors, but the ‘misquotation’ exists, too, and they can be misleading to the reader. The aim of this study was to examine the quotation accuracy of SR protocols in a meta-analysis on acupuncture research. We searched SCOPUS through 31 December, 2020, and each protocol and its citations were analyzed and classified as correct or incorrect. We used descriptive statistics to report the quotation errors and characteristics of the included protocols. The results showed 248 SR protocols, where 124 protocols received quotations and 38 quotations (31.4%) were erroneous. Only 11 (4.4%) of the published SRs and SR protocols had been published previously. Furthermore, the scientific journal in which the most SR protocols were published was Medicine (193; 77.8%), followed by BMJ Open (39; 15.7%). Authors from China (86.5%) were the most productive in publishing SRs and SR protocols. Finally, we concluded that the number of SR protocols and meta-analyses published in scientific journals and indexed by databases exceeds the publication capacity of the SRs associated with them, generating scientific literature that does not make any novel contribution to knowledge.

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“…POF is characterized by premature depletion of ovarian follicles in approximately 3% of women younger than 40 years old which is diagnosed by high levels of gonadotropins such as luteinizing hormone (LH) and FSH, and low levels of gonadal hormones including estrogens and inhibins [27,28]. Interestingly, the administration of ICI to animal models of D-galactose-induced POF caused a significant reduction in LH and FSH levels, promotion of estrogen and anti-Müllerian hormone (AMH) production, and increased viability of granulosa cells, which were all assumed to be the result of strengthening the DNA repair system indicated by altered levels γH2AX and 53BP1 expression upon treatment with ICI [29].…”

Section: Icariin Modulates Reproductive Hormones Synthesismentioning

confidence: 99%

Icariin, A Novel Promising Complementary Therapeutic Strategy In The Management Of Female Infertility: A Literature Review

Mosavi

Rostami

Pooladi

et al. 2023

GMJ

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The prevalence of pre-menopausal female infertility is increasing considerably due to various causes such as environmental pollutants, increased administration of chemotherapeutics and radiation exposure, microbial infections, and genetic/epigenetic alterations. However, the current therapeutical strategies remain unfavorite as the disadvantages are strongly challenging. Icariin (ICI) is a phytoestrogen that exerts some promising properties in order to alleviate female infertility. Therefore, the current literature review aimed to evaluate the conducted studies regarding the beneficial impacts of ICI on the female reproductive system and female fecundity. The findings of the present study revealed that ICI is able to modulate the levels of reproductive hormones as it causes a significant decrement in the levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) while increasing the levels of estrogen and progesterone. Furthermore, the administration of ICI results in a dramatic alteration in the expression of sex steroids’ receptors, particularly in female reproductive tissues. In addition, preserving ovarian follicular reserve, improving the ovarian and uterine histoarchitecture, elongating the estrous cycle duration, and eventually advancing the female fecundity are other major effects of ICI on the female reproductive system. Despite these desired beneficial properties, the current knowledge appears to be insufficient, hence further investigations, particularly on humans, are encouraged. To the best of our knowledge, this review provides a comprehensive information regarding the beneficial effects of Icariin on female infertility for the firs time

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The Treatment of Complementary and Alternative Medicine on Premature Ovarian Failure

Cited by 22 publications

References 59 publications

miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4

miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4

Quotation Accuracy of Systematic Review and Meta-Analysis Protocols on Acupuncture

Icariin, A Novel Promising Complementary Therapeutic Strategy In The Management Of Female Infertility: A Literature Review

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