The Tube Method of Measuring the Growth Rate of Neurospora

Genes encoding a striatin-like protein (ham-3) and a forkhead associated protein (ham-4) are required for hyphal fusion in Neurospora crassa

“…The strain FGSC 4564 was used as a helper for crosses when the female could not produce sexual structures (Perkins, 1984). Growth rates were assessed using the race tube method (Ryan et al, 1943). Electroporation was performed according to (Margolin et al, 1997) with 1.5 kV setting.…”

Kinetics of circadian band development in Neurospora crassa

“…Ryan et al (1943) also found slower growth rates in cultures of N. crassa grown on race tubes when smaller volumes of agar medium were used. Despite diVerences in methodology, strain, and rates of elongation, the overall decrease observed by Sargent et al (1966) (approximately 43%) agrees with the decrease of about 42% measured on microscope slide cultures recorded by Castro-Longoria et al (2004).…”

Neurospora crassa homologue of Neuronal Calcium Sensor-1 has a role in growth, calcium stress tolerance, and ultraviolet survival

“…Growth was initially measured by placing either conidia or a plug of agar containing mycelium in the center of a petri dish and colony diameter was measured every 2-3 h to obtain linear rates of diameter increase over a period of 28 h. Strains that show lower growth rate on (Prakash et al 1999) 21. 34-97-3a upr-1 NCREV3 Laboratory stock (Tamuli et al 2006) petri dish were further analyzed by using standard race tube assay (Ryan et al 1943;Ryan 1950). Growth rates were calculated as cm h -1 in both cases.…”