The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis

Zhang, Shumeng; Li, Xinfeng; Wang, Xun; Li, Zhou; He, Jin

doi:10.1007/s00203-016-1239-z

Cited by 19 publications

(10 citation statements)

References 24 publications

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“…In addition, there was considerable allelic variation in a number of transcriptional regulators, including members of the TetR, DeoR, and GntR families. In addition, there was allelic variation in the YvcQ sensor kinase genes of the YvcPQ two-component regulatory system, which is involved in mediating resistance to bacitracin in Bacillus species (37,38). These data indicate that variation in alleles associated with transcriptional regulation, and expected alterations in transcriptional control, may be an important mediator of the tissue tropisms of B. cereus.…”

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confidence: 84%

Whole-Genome Characterization of Bacillus cereus Associated with Specific Disease Manifestations

Chang

Rosch

et al. 2018

Infect Immun

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remains an important cause of infections, particularly in immunocompromised hosts. While typically associated with enteric infections, disease manifestations can be quite diverse and include skin infections, bacteremia, pneumonia, and meningitis. Whether there are any genetic correlates of bacterial strains with particular clinical manifestations remains unknown. To address this gap in understanding we undertook whole genome analysis of strains isolated from patients with a range of disease manifestations, including non-invasive colonizing disease, superficial skin infections, and invasive bacteremia. Interestingly, strains involved in skin infection tended to form a distinct genetic cluster compared to isolates associated with invasive disease. Other disease manifestations, despite not being exclusively clustered, nonetheless had unique genetic features. The unique features associated with the specific types of infections ranged from traditional virulence determinants, metabolic pathways, and gene regulators. These data represent the largest genetic analysis to-date of pathogenic isolates with associated clinical parameters.

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confidence: 84%

Whole-Genome Characterization of Bacillus cereus Associated with Specific Disease Manifestations

Chang

Rosch

et al. 2018

Infect Immun

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“…Bacillus subtilis, a soil-dwelling model organism, increases expression of BceAB, an ABC-family efflux pump, via its TCS BceRS in response to exposure to the antibiotic bacitracin [65,66]. Similarly, the YvcPQ TCS in Bacillus thuringiensis, a member of the Bacillus cereus group, modulates efflux via two ABC transporters in response to lantibiotics and bacitracin [221].…”

Section: Gram-negative Bacteriamentioning

confidence: 99%

Roles of Two-Component Regulatory Systems in Antibiotic Resistance

2019

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Two-component regulatory systems (TCSs) are a major mechanism by which bacteria sense and respond to changes in their environment. TCSs typically consist of two proteins that bring about major regulation of the cell genome through coordinated action mediated by phosphorylation. Environmental conditions that activate TCSs are numerous and diverse and include exposure to antibiotics as well as conditions inside a host. The resulting regulatory action often involves activation of antibiotic defenses and changes to cell physiology that increase antibiotic resistance. Examples of resistance mechanisms enacted by TCSs contained in this review span those found in both Gram-negative and Gram-positive species and include cell surface modifications, changes in cell permeability, increased biofilm formation, and upregulation of antibiotic-degrading enzymes.

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“…If these unsure knockout strains were chosen for genetic study, inconsistent conclusions could be drawn. Based on our practice in the deletion of hundreds of genes in BMB171, we concluded that this system is easy to operate and allows for clean deletions of one or more genes within an operon ( Zheng et al, 2015 ; Zhang et al, 2015 , 2016 ). Because there is no need for a higher screening temperature, undesirable mutations could decrease, resulting with much more consistent results.…”

Section: Discussionmentioning

confidence: 99%

Poly-β-hydroxybutyrate Metabolism Is Unrelated to the Sporulation and Parasporal Crystal Protein Formation in Bacillus thuringiensis

Wang

Zhou

et al. 2016

Front. Microbiol.

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Poly-3-hydroxybutyrate (PHB) is a natural polymer synthesized by many bacteria as a carbon-energy storage material. It was accumulated maximally prior to the spore formation but was degraded during the process of sporulation in Bacillus thuringiensis. Intriguingly, B. thuringiensis also accumulates large amounts of insecticidal crystal proteins (ICPs) during sporulation, which requires considerable input of carbon and energy sources. How PHB accumulation affects sporulation and ICP formation remains unclear to date. Intuitively, one would imagine that accumulated PHB provides the energy required for ICP formation. Yet our current data indicate that this is not the case. First, growth curves of the deletion mutants of phaC (encoding the PHB synthase) and phaZ (encoding the PHB depolymerase) were found to be similar to the parent strain BMB171; no difference in growth rate could be observed. In addition we further constructed the cry1Ac10 ICP gene overexpression strains of BMB171 (BMB171-cry), as well as its phaC and phaZ deletion mutants ΔphaC-cry and ΔphaZ-cry to compare their spore and ICP production rates. Again, not much change of ICP production was observed among these strains either. In fact, PHB was still degraded in most ΔphaZ-cry cells as observed by transmission electron microscopy. Together these results indicated that there is no direct association between the PHB accumulation and the sporulation and ICP formation in B. thuringiensis. Some other enzymes for PHB degradation or other energy source may be responsible for the sporulation and/or ICP formation in B. thuringiensis.

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The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis

Cited by 19 publications

References 24 publications

Whole-Genome Characterization of Bacillus cereus Associated with Specific Disease Manifestations

Whole-Genome Characterization of Bacillus cereus Associated with Specific Disease Manifestations

Roles of Two-Component Regulatory Systems in Antibiotic Resistance

Poly-β-hydroxybutyrate Metabolism Is Unrelated to the Sporulation and Parasporal Crystal Protein Formation in Bacillus thuringiensis

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