The two domains of cotton WLIM1a protein are functionally divergent

Han, Libo; Li, Yuanbao; Sun, Yong‐Duo; Wang, Haiyun; Kong, Zhaosheng; Xia, Gui-Xian

doi:10.1007/s11427-016-5002-0

Cited by 10 publications

(4 citation statements)

References 25 publications

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“…However, more variation was found in their C‐terminal LIM domain. Recently, a study indicated that the N‐terminal LIM domain contributes primarily to the F‐actin bundling activity of GhWLIM1a, but the C‐terminal LIM domain contributes to the DNA‐binding activity (Han et al ., ). So, the difference sequence between GhWLIM1a and GhXLIM6 C‐terminal LIM domain may lead to their functional differentiation in fiber cell development.…”

Section: Discussionmentioning

confidence: 97%

The cotton XLIM protein (GhXLIM6) is required for fiber development via maintaining dynamic F‐actin cytoskeleton and modulating cellulose biosynthesis

Wang

et al. 2018

The Plant Journal

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N.-N.W. contributed equally to this work.SUMMARY LIM domain proteins are cysteine-rich proteins, and are often considered as actin bundlers and transcription factors in plants. However, the roles of XLIM proteins in plants (especially in cotton) remain unexplored in detail so far. In this study, we identified a cotton XLIM protein (GhXLIM6) that is preferentially expressed in cotton fiber during whole elongation stage and early secondary cell wall (SCW) synthesis stage. The GhXLIM6-silenced transgenic cotton produces shorter fibers with thinner cell walls, compared with wildtype (WT). GhXLIM6 protein could directly bind F-actin and promote actin polymerization both in vitro and in vivo. It also acts as a transcription factor to suppress GhKNL1 expression through binding the PAL-box element of GhKNL1 promoter, and subsequently regulate the expression of CesA genes related to cellulose biosynthesis and deposition in SCWs of cotton fibers. The cellulose content in fibers of GhXLIM6 RNAi cotton is lower than that in WT. Taken together, these data reveal the dual roles of GhXLIM6 in fiber development. On one hand, GhXLIM6 functions in fiber elongation through binding to F-actin to maintain the dynamic F-actin cytoskeleton. On the other hand, GhXLIM6 fine-tunes fiber SCW formation, probably through directly suppressing transcription of GhKNL1 to promote cellulose biosynthesis.

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Section: Discussionmentioning

confidence: 97%

The cotton XLIM protein (GhXLIM6) is required for fiber development via maintaining dynamic F‐actin cytoskeleton and modulating cellulose biosynthesis

Wang

et al. 2018

The Plant Journal

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“…A typical plant LIM protein contains two LIM domains, and the two domains were shown to occupy different actin affinities. For example, LIM1 of cotton WLIM1a protein contributed primarily to the actin filament-bundling activity, whereas LIM2 contributed to the DNA-binding activity of the protein [ 36 ]. Differently from our experiment, it seems that both LIM1 and LIM2 of GhWLIM1C could bind to the actin filaments ( Figure 3 B,C; Figure 5 B,C); while LIM1 only possesses actin-binding activity ( Figure 5 B), LIM2 could directly bind and bundle actin filaments ( Figure 5 C).…”

Section: Discussionmentioning

confidence: 99%

Silencing of a Cotton Actin-Binding Protein GhWLIM1C Decreases Resistance against Verticillium dahliae Infection

Cao

Qin

Zhu

et al. 2022

Plants

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LIM proteins are widely spread in various types of plant cells and play diversely crucial cellular roles through actin cytoskeleton assembly and gene expression regulation. Till now, it has not been clear whether LIM proteins function in plant pathogen defense. In this study, we characterized a LIM protein, GhWLIM1C, in upland cotton (Gossypium hirsutum). We found that GhWLIM1C could bind and bundle the actin cytoskeleton, and it contains two LIM domains (LIM1 and LIM2). Both the two domains could bind directly to the actin filaments. Moreover, the LIM2 domain additionally bundles the actin cytoskeleton, indicating that it possesses a different biochemical activity than LIM1. The expression of GhWLIM1C responds to the infection of the cotton fungal pathogen Verticillium dahliae (V. dahliae). Silencing of GhWLIM1C decreased cotton resistance to V. dahliae. These may be associated with the down regulated plant defense response, including the PR genes expression and ROS accumulation in the infected cotton plants. In all, these results provide new evidence that a plant LIM protein functions in plant pathogen resistance and the assembly of the actin cytoskeleton are closely related to the triggering of the plant defense response.

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“…Plant LIM proteins are another important class of ABPs [ 65 ], which are found to be dispersed in the cytosol and nucleoplasm. The LIM-domain-containing proteins in the nucleus are preferentially involved in tissue-specific gene regulation and determination of cell fate, whereas the cytoplasmic LIM-domain-containing proteins are involved mainly in cytoskeletal organization [ 66 ]. The term “LIM” originates from the initials LIN-11, ISL-1, and MEC-3, the first proteins observed to contain this particular homeodomain.…”

Section: Role Of Actin Genes In Cotton Fiber Devel...mentioning

confidence: 99%

Role of Actin Dynamics and GhACTIN1 Gene in Cotton Fiber Development: A Prototypical Cell for Study

Iqbal,

Aslam,

Ahmed

et al. 2023

Genes

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Cotton crop is considered valuable for its fiber and seed oil. Cotton fiber is a single-celled outgrowth from the ovule epidermis, and it is a very dynamic cell for study. It has four distinct but overlapping developmental stages: initiation, elongation, secondary cell wall synthesis, and maturation. Among the various qualitative characteristics of cotton fiber, the important ones are the cotton fiber staple length, tensile strength, micronaire values, and fiber maturity. Actin dynamics are known to play an important role in fiber elongation and maturation. The current review gives an insight into the cotton fiber developmental stages, the qualitative traits associated with cotton fiber, and the set of genes involved in regulating these developmental stages and fiber traits. This review also highlights some prospects for how biotechnological approaches can improve cotton fiber quality.

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The two domains of cotton WLIM1a protein are functionally divergent

Cited by 10 publications

References 25 publications

The cotton XLIM protein (GhXLIM6) is required for fiber development via maintaining dynamic F‐actin cytoskeleton and modulating cellulose biosynthesis

The cotton XLIM protein (GhXLIM6) is required for fiber development via maintaining dynamic F‐actin cytoskeleton and modulating cellulose biosynthesis

Silencing of a Cotton Actin-Binding Protein GhWLIM1C Decreases Resistance against Verticillium dahliae Infection

Role of Actin Dynamics and GhACTIN1 Gene in Cotton Fiber Development: A Prototypical Cell for Study

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