The Ty1 Retrotransposition Short-Term Test for Selective Detection of Carcinogenic Genotoxins

Stoycheva, Teodora; Pesheva, M.; Dimitrov, Milen; Venkov, Pencho

doi:10.5772/38095

Cited by 5 publications

(3 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main advantage of the Ty1antiROS test consists in measuring antioxidant activity by following the changes of a cellular process, the Ty1 transposition, instead of using chemical reactions. Transposones are ubiquitous, from E.coli to humans, and changes in their transposition are regulated by hundreds of gene functions involved in the main cellular processes . In the Ty1antiROS test the oxidative species are produced by tester cells and the interaction with antioxidant takes place in live cells.…”

Section: Discussionmentioning

confidence: 99%

“…Transposones are ubiquitous, from E.coli to humans, and changes in their transposition are regulated by hundreds of gene functions involved in the main cellular processes. 42 In the Ty1antiROS test the oxidative species are produced by tester cells and the interaction with antioxidant takes place in live cells. The assay shows good sensitivity, as evidenced by its ability to detect antioxidant activity in royal jelly (Table 2), which is often impossible with some other cellbased assays.…”

Section: ■ Discussionmentioning

confidence: 99%

See 1 more Smart Citation

New Cell-Based Assay Indicates Dependence of Antioxidant Biological Activity on the Origin of Reactive Oxygen Species

Dimitrov

Pesheva

Venkov

2013

J. Agric. Food Chem.

View full text Add to dashboard Cite

The mobility of the Ty1 transposon in Saccharomyces cerevisiae was found to vary proportionally with the level of ROS generated in cells, which provides the possibility to determine antioxidant activity by changes in a cellular process instead of using chemical reactions. The study of propolis, royal jelly, and honey with the newly developed Ty1antiROS test reveals an inverse exponential dependence of antioxidant activity on increased concentrations. This dependence can be transformed to proportional by changing the source of ROS: instead of cell-produced to applied as hydrogen peroxide. The different test responses are not due to excess of added hydrogen peroxide, as evidenced by the exponential dependence found by usage of yap1Δ tester cells accumulating cell-generated ROS. Results indicate that the activity of antioxidants to oxidative radicals depends on the origin of ROS, and this activity is elevated for cell-generated ROS compared to ROS added as reagents in the assay.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

New Cell-Based Assay Indicates Dependence of Antioxidant Biological Activity on the Origin of Reactive Oxygen Species

Dimitrov

Pesheva

Venkov

2013

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Appropriate dilutions of cells were then placed on YEPD to evaluate cell survival and on selective medium lacking histidine -for His + transposants. Yeast media were prepared as described by [27]. has a disrupted YAP1 gene and was constructed by transformation of 551 cells with the yap1:: hisG-URA3-hisG cassette; the strain is characterized with a deletion of the yap1 gene, which makes this strain incapable of synthesizing yap1 protein [25] Mean transposition rates were determined and results presented as "fold increase of Ty1 transposition rate" related to control sample, taken as 1.00.…”

Section: Ty1 Transposition Assaymentioning

confidence: 99%

Oxidal® Ameliorates the Ty1 Retrotransposition Induced by Methyl Methanesulfonate in Saccharomyces cerevisiae

Todorova

Dimitrov

Ignatov

et al. 2020

MRJI

Self Cite

View full text Add to dashboard Cite

Aim: The aim of the study was to evaluate the potential of Oxidal® to decrease the Ty1 retrotransposition rate in a model system Saccharomyces cerevisiae. Study Design: Saccharomyces cerevisiae cell suspensions were pre-treated with different concentrations Oxidal® and subsequently treated with 16mM methyl methanesulfonate. (MMS) Methodology: The potential of various concentrations Oxidal® was evaluated based on “spot” test and Ty1 retro-transposition test. Results: Data revealed that only 5% Oxidal® possesses some cytotoxic properties. Lack of Ty1 retro-transposition was observed after single treatment with 1, 2.5 and 5% Oxidal® concentrations. On the other hand, all the tested concentrations showed promising results against the standard carcinogen methyl methane sulfonate. The most pronounced anti-carcinogenic and cytoprotective effects were observed after pre-treatment with 2.5% Oxidal®, which could be attributed to the antioxidant properties of the combination of ingredients; methylene blue, salicylic acid and caffeine. Further studies could reveal the exact mechanism of action of the supplement and the role of the antioxidant potential. Conclusion: New data is provided concerning the potential of Oxidal® at low concentrations to protect Saccharomyces cerevisiae cells from MMS-induced Ty1 retro-transposition. The cytoprotective properties of the supplement were also obtained. These results could be considered as a basis for further studies revealing the exact mechanisms of cell protection of the Oxidal®. Additionally, our data could also serve as an important step of the in-depth research of a potential antiviral activity.

show abstract

Apricot kernels’ extract and amygdalin alter bleomycin-induced Ty1 retrotransposition, mitotic gene conversion in the trp-5 locus and reverse point mutations in ilv1-92 allele in Saccharomyces cerevisiae

Todorova

2022

Arch Microbiol

View full text Add to dashboard Cite

The Ty1 Retrotransposition Short-Term Test for Selective Detection of Carcinogenic Genotoxins

Cited by 5 publications

References 79 publications

New Cell-Based Assay Indicates Dependence of Antioxidant Biological Activity on the Origin of Reactive Oxygen Species

New Cell-Based Assay Indicates Dependence of Antioxidant Biological Activity on the Origin of Reactive Oxygen Species

Oxidal® Ameliorates the Ty1 Retrotransposition Induced by Methyl Methanesulfonate in Saccharomyces cerevisiae

Apricot kernels’ extract and amygdalin alter bleomycin-induced Ty1 retrotransposition, mitotic gene conversion in the trp-5 locus and reverse point mutations in ilv1-92 allele in Saccharomyces cerevisiae

Contact Info

Product

Resources

About