The Ty1 transposition assay: a new short-term test for detection of carcinogens

The Ty1 Retrotransposition Short-Term Test for Selective Detection of Carcinogenic Genotoxins

“…The positive answer of Ty1 test is not due to the stress conditions generated by the increased cellular toxicity at higher concentrations of B(a)P, since treatment with NaN 3 , a powerful cell poison, had no effect on Ty1 transposition. Similar positive responses of the Ty1 test were found in concentration dependent and kinetic experiments with a number of carcinogenic genotoxins showing low response in tests based on wild type of yeast cells (Dimitrov et al, 2011;Pesheva et al, 2005;Staleva & Venkov 2001).…”

“…The percentage of cells that survived this treatment decreases with increasing B(a)P concentration suggesting that ones inside cells the pre-carcinogen has a toxic effect which however is not an inducer of Ty1 transposition. The Ty1 test was positive with B(a)P and different other pre-carcinogens only in presence of S9 metabolizing mix (Dimitrov et al 2011;Pesheva et al 2005;. These results evidence that the sec53 mutation in S. cerevisiae 551 tester strain increases the sensitivity of Ty1 test due to increased permeability of cells.…”

Ty1 transposition induced by carcinogens in Saccharomyces cerevisiae yeast depends on mitochondrial function

“…The decrease, or, in most cases, absence, of successful Ty1 transposition in rho − cells (Table 1) was remarkable since all of (Pesheva et al, 2005, this study) and (ii) belonged to groups of carcinogens with quite different mechanisms of action. This suggested that the choice of rho + cells for a successful Ty1 transposition is not due to the individual properties of the carcinogenic substances tested and might instead reflect a common characteristic: their carcinogenicity.…”

“…The derivative ts1 (Pesheva et al, 2005) of S. cerevisiae strain DG1141 (Curcio and Garfinkel, 1999) Matα ura3-167 his3Δ200::Tym His3-AI was used.…”

“…Recently it has been found that laboratory carcinogens also induce Ty1 transposition (Pesheva et al, 2005). The process of carcinogen induced Ty1 transposition depends on the function of the RAD9 gene and transit through the G1 phase of the yeast cell cycle (Staleva and Venkov, 2001).…”