The Type 2C protein phosphatase FgPtc1p of the plant fungal pathogen <i>Fusarium graminearum</i> is involved in lithium toxicity and virulence

Jiang, Lijing; Yang, Jingran; Feng, Fan; Zhang, Dajun; Xuli, Wang

doi:10.1111/j.1364-3703.2009.00598.x

Cited by 22 publications

(10 citation statements)

References 36 publications

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“…Although FgTep1p and ScTep1p only share 26% identity in overall amino acid sequence, they contain a conserved PTPc domain that is critical for phosphatase activity. This is the case for the F. graminearum Type 2C protein phosphatase FgPtc1p, which also shares a limited primary amino acid sequence homology (25%), but a conserved PP2C catalytic domain, with ScPtc1p, and therefore complements the function of ScPtc1p in lithium and calcium sensitivities (Jiang et al . 2010).…”

Section: Resultsmentioning

confidence: 99%

FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat

Zhang¹,

Feng²,

Yang³

et al. 2010

Molecular Plant Pathology

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Both mammalian tensin-like phosphatase 1 [TEP1; also known as phosphatase deleted on chromosome 10 (PTEN) or mutated in multiple advanced cancer 1 (MMAC1)] and Saccharomyces cerevisiae ScTep1p are involved in the phosphatidylinositol pathway. In this study, we identified the Fusarium graminearum locus FGSG_04982.3 (named FgTEP1) as the functional homologue of ScTEP1 in the sensitivity of S. cerevisiae cells to wortmannin, the phosphatidylinositol-3 kinase inhibitor. Deletion of FgTEP1 causes F. graminearum mycelial growth to become sensitive to lithium and reduces the production of conidia. Although conidia lacking FgTEP1 germinate normally, they show reduced germination efficiency in the presence of wortmannin. In addition, we showed that deletion of FgTEP1 reduces the virulence of F. graminearum on wheat. These results indicate that FgTep1p is linked to the phosphatidylinositol-3 kinase signalling pathway in this plant fungal pathogen.

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Section: Resultsmentioning

confidence: 99%

FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat

Zhang¹,

Feng²,

Yang³

et al. 2010

Molecular Plant Pathology

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“…Fusarium graminearum (also known as Gibberella zeae) is the major causal agent of Fusarium head blast disease on wheat and barley, which causes yield losses and affects the quality of grains. Using ScPtc1 as a query, Jiang et al identified a single homologue sequence, encoded by a gene named FgPTC1, in the genome of F. graminearum (42). FgPtc1 (no.…”

Section: Downloaded Frommentioning

confidence: 99%

Type 2C Protein Phosphatases in Fungi

2011

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Type 2C Ser/Thr phosphatases are a remarkable class of protein phosphatases, which are conserved in eukaryotes and involved in a large variety of functional processes. Unlike in other Ser/Thr phosphatases, the catalytic polypeptide is not usually associated with regulatory subunits, and functional specificity is achieved by encoding multiple isoforms. For fungi, most information comes from the study of type 2C protein phosphatase (PP2C) enzymes in Saccharomyces cerevisiae, where seven PP2C-encoding genes (PTC1 to -7) with diverse functions can be found. More recently, data on several Candida albicans PP2C proteins became available, suggesting that some of them can be involved in virulence. In this work we review the available literature on fungal PP2Cs and explore sequence databases to provide a comprehensive overview of these enzymes in fungi.Reversible phosphorylation of proteins is a major mechanism regulating many biological processes such as metabolism, gene transcription, or cell cycle. It is an extremely common event in signal transduction, and it is considered the main mechanism of posttranslational modification leading, for instance, to a change in enzyme activity. The phosphorylation state of a protein results from the balance of protein kinases and protein phosphatases activities. Alterations in the phosphorylation state of proteins are a cause of various diseases, such as cancer, diabetes, rheumatoid arthritis, or hypertension. The importance of this regulatory mechanism is evident when it is considered that it affects around 30% of the proteome of Saccharomyces cerevisiae (72) and that the number of genes encoding phosphatases and kinases represents 2 to 4% of all genes in a typical eukaryotic genome (60).Most phosphorylation events in eukaryotes involve transfer of phosphate to Ser and Thr residues. Removal of this phosphate is catalyzed by Ser/Thr protein phosphatases. Members of this large family of enzymes were initially classified, using enzymological criteria, as type 1 (PP1) and type 2 (PP2) phosphatases. PP2 enzymes were subsequently divided into three groups on the basis of the requirements for metal ions: 2A (not requiring metal ions), 2B (activated by calcium), and 2C (Mg 2ϩ dependent) (10). The molecular cloning of a number of cDNAs and genes from diverse organisms allowed the establishment of three major subfamilies: PPP (phosphoprotein phosphatases), including type 1, 2A, and 2B phosphatases, among others; PPM (metal-dependent protein phosphatases), including type 2C enzymes (PP2C), which are the subject of this review; and the catalytically Asp-based subfamily, represented by HAD and FCP/SCP (see reference 89 for a review).Although PP2C proteins are distantly related in primary sequence to PPP enzymes, their tridimensional structure and catalytic mechanisms appear to be quite similar. Type 2C phosphatases are widely represented in bacteria, fungi, plants, insects, and mammals (83), suggesting that they are involved in key cellular processes, such as proliferation, metabolism, and cell...

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“…In Fusarium graminearum , the major causal agent of Fusarium head blast disease on barley and wheat, PTC1 was found to play an important role in the ability of mycelial growth to resist lithium toxicity. 221 Deletion of PTC1 attenuates F. graminearum virulence on wheat coleoptiles but not on wheat heads. 221 , 222 While these results may suggest that mycelial growth mediated by Ptc1 plays a specialized role in directing virulence against specific niches on wheat, another possible explanation is that independently constructed versions of the ptc1 deletion strain were used to test for virulence in the wheat coleoptile vs. head models.…”

Section: Introductionmentioning

confidence: 99%

“… 221 Deletion of PTC1 attenuates F. graminearum virulence on wheat coleoptiles but not on wheat heads. 221 , 222 While these results may suggest that mycelial growth mediated by Ptc1 plays a specialized role in directing virulence against specific niches on wheat, another possible explanation is that independently constructed versions of the ptc1 deletion strain were used to test for virulence in the wheat coleoptile vs. head models. We conclude that PP2C phosphatases play an integral role in an array of key cellular processes in pathogenic and non-pathogenic fungi, including cell wall integrity, filamentous growth, and virulence.…”

Section: Introductionmentioning

confidence: 99%

Regulatory roles of phosphorylation in model and pathogenic fungi

Bataineh

Kadosh

2015

Med. Myco.

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Over the past 20 years, considerable advances have been made toward our understanding of how post-translational modifications affect a wide variety of biological processes, including morphology and virulence, in medically important fungi. Phosphorylation stands out as a key molecular switch and regulatory modification that plays a critical role in controlling these processes. In this article, we first provide a comprehensive and up-to-date overview of the regulatory roles that both Ser/Thr and non-Ser/Thr kinases and phosphatases play in model and pathogenic fungi. Next, we discuss the impact of current global approaches that are being used to define the complete set of phosphorylation targets (phosphoproteome) in medically important fungi. Finally, we provide new insights and perspectives into the potential use of key regulatory kinases and phosphatases as targets for the development of novel and more effective antifungal strategies.

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The Type 2C protein phosphatase FgPtc1p of the plant fungal pathogen Fusarium graminearum is involved in lithium toxicity and virulence

Cited by 22 publications

References 36 publications

FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat

FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat

Type 2C Protein Phosphatases in Fungi

Regulatory roles of phosphorylation in model and pathogenic fungi

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