The Ultrastructure of Cultivable Treponemes

Hougen, K. Hovind

doi:10.1111/j.1699-0463.1975.tb00076.x

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…Media such as Papillomatous digital dermatitis Treponema (PDDTp) [82,83] and Thioglycolate medium [84] have a formulation favouring amino acids and peptides. The notable difference between the composition of both media is the absence of growth factors in PDDTp and the absence of proper SCFA input, probably explaining the higher yield of Thioglycolate medium (18 % versus 4 %) [82,83,[85][86][87] (Fig. 1).…”

Section: Resulting Media Formulated Specifically For Treponema and Their Input In The Treponemal Diversitymentioning

confidence: 99%

A review of in vitro attempts to develop the axenic culture of Treponema pallidum and genomics-based suggestions to achieve this elusive goal

Belkacemi

Alou

Khelaifia

et al. 2021

Journal of Medical Microbiology

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To date, the axenic culture of Treponema pallidum remains a challenge in the field of microbiology despite countless attempts. Here, we conducted a comprehensive bibliographic analysis using several databases and search engines, namely Pubmed, Google scholar, Google, Web of Science and Scopus. Numerous unsuccessful empiric studies have been conducted and evaluated using as criteria dark-field microscopic observation of motile spiral shaped cells in the culture and virulence of the culture through rabbit infectivity. All of these studies failed to induce rabbit infectivity, even when deemed positive after microscopic observation leading to the misnomer of avirulent T. pallidum . In fact, this criterion was improperly chosen because not all spiral shaped cells are T. pallidum . However, these studies led to the formulation of culture media particularly favourable to the growth of several species of Treponema, including Oral Microbiology and Immunology, Zürich medium (OMIZ), Oral Treponeme Enrichment Broth (OTEB) and T-Raoult, thus allowing the increase in the number of cultivable strains of Treponema . The predicted metabolic capacities of T. pallidum show limited metabolism, also exhibited by other non-cultured and pathogenic Treponema species, in contrast to cultured Treponema species. The advent of next generation sequencing represents a turning point in this field, as the knowledge inferred from the genome can finally lead to the axenic culture of T. pallidum .

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Section: Resulting Media Formulated Specifically For Treponema and Their Input In The Treponemal Diversitymentioning

confidence: 99%

A review of in vitro attempts to develop the axenic culture of Treponema pallidum and genomics-based suggestions to achieve this elusive goal

Belkacemi

Alou

Khelaifia

et al. 2021

Journal of Medical Microbiology

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“…Treponemes are spiral-shaped motile prokaryotes belonging to the genus Treponema and the order Spirochaetales, in the family Spirochaetaceae [61,62]. These microorganisms colonise the human intestinal [63], oral [64] and genital [65] microbiota, as well as those of animals. Most of these microorganisms are part of the commensal microbiota, but some species are opportunistic pathogens, such as Treponema pallidum, the causative agent of syphilis in humans [66].…”

Section: Treponemes Of the Oral Cavitymentioning

confidence: 99%

Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques

et al. 2023

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Recent years have been marked by a paradigm shift in the study of the human microbiota, with a re-emergence of culture-dependent approaches. Numerous studies have been devoted to the human microbiota, while studies on the oral microbiota still remain limited. Indeed, various techniques described in the literature may enable an exhaustive study of the microbial composition of a complex ecosystem. In this article, we report different methodologies and culture media described in the literature that can be applied to study the oral microbiota by culture. We report on specific methodologies for targeted culture and specific culture techniques and selection methodologies for cultivating members of the three kingdoms of life commonly found in the human oral cavity, namely, eukaryota, bacteria and archaea. This bibliographic review aims to bring together the various techniques described in the literature, enabling a comprehensive study of the oral microbiota in order to demonstrate its involvement in oral health and diseases.

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Characterization of the cytoplasmic fibrils of Treponema refringens (Nichols)

Eipert

Black

1979

Arch. Microbiol.

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The cytoplasmic fibrils of Treponema refringens were studied in situ by electron microscopy of thin sectioned and negatively stained cells. From 5 to 21 parallel fibrils ran through the cell in a band adjacent to the inner side of the cytoplasmic membrane, on the inner sides of the curves of the spirochete. The nuclear areas of cells were adjacent to the fibrils. Cross sections of fibrils isolated from cells which had been lysed were polygonal and not uniformly electron dense. Polyacrylamide gel electrophoresis of partially purified fibril preparations indicated their main component to be a protein with a molecular weight of 97,000. Fibrils were solubilized by 1% trypsin, 1% pronase, 6 M urea, 1 N HCl, 0.005 N NaOH or 1.3% sodium dodecyl sulfate. By electron microscopy of negatively stained isolated fibrils, each fibril was found to be a complex arrangement of strands rather than a single tubule.

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The Ultrastructure of Cultivable Treponemes

Cited by 6 publications

References 11 publications

A review of in vitro attempts to develop the axenic culture of Treponema pallidum and genomics-based suggestions to achieve this elusive goal

A review of in vitro attempts to develop the axenic culture of Treponema pallidum and genomics-based suggestions to achieve this elusive goal

Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques

Characterization of the cytoplasmic fibrils of Treponema refringens (Nichols)

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