The ultrastructure ofAcetivibrio cellulolyticus, a recently isolated cellulolytic anaerobe

Colvin, J. Ross; Sowden, L. C.; Patel, G. B.; Khan, Ayesha

doi:10.1007/bf01570973

Cited by 8 publications

(8 citation statements)

References 9 publications

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“…Altogether, including the resident ScaA enzyme, 96 different enzyme molecules can theoretically be incorporated into each A. cellulolyticus cellulosome. The intricate nature of the putative supramolecular complex is consistent with the exceptionally elaborate cell surface architecture of this bacterium (13,34,35). The central factor contributing to the enhanced amplification of the A. cellulolyticus system is the ScaB adaptor protein.…”

Section: Vol 185 2003mentioning

confidence: 54%

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The Cellulosome System of Acetivibrio cellulolyticus Includes a Novel Type of Adaptor Protein and a Cell Surface Anchoring Protein

Gao

Ding

et al. 2003

J Bacteriol

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The microbial degradation of cellulose is one of the most important processes on Earth, and it affects the human condition in many direct and indirect ways. If it did not occur, there would be an inexhaustible accumulation of plant cell refuse, and herbivorous life forms would largely vanish.The multienzyme cellulosome complex is a major mechanism by which some cellulolytic bacteria efficiently degrade cellulose and related plant cell wall polysaccharides (2,4,5,8,9,18,20,32,58,59). To date, cellulosomes have been found in several strains of anaerobic bacteria and fungi obtained from very different types of ecosystems. The first cellulosome was discovered in studies on the anaerobic thermophile Clostridium thermocellum (3,36,37). The cellulosome system of this organism consists of a variety of different enzymes bound to a noncatalytic scaffoldin subunit, which can, in turn, bind to one of several cell surface anchoring proteins. In this organism, both the attachment of the enzymes to the scaffoldin and the attachment of the scaffoldin to the anchoring proteins are accomplished by a special kind of protein-protein interaction, the cohesin-dockerin interaction. In this context, the enzyme subunits include a dockerin domain, and the scaffoldin contains multiple copies of cohesin modules for collective incorporation into the complex. The scaffoldin subunit itself harbors a single dockerin variant that interacts selectively with corresponding cohesin variants on the anchoring proteins. In C. thermocellum, the primary scaffoldin is trifunctional in that it also contains (in addition to the cohesins and dockerin) a substrate-targeting cellulose-binding domain (CBD). The anchoring proteins are bifunctional; in addition to the cohesins, they have another type of domain, the S-layer homology domain (SLH), which is known to bind strongly to the cell surface (12,40). Thus, the sequential cohesin-dockerin-mediated set of interactions among the enzymes, scaffoldin, and anchoring

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Section: Vol 185 2003mentioning

confidence: 54%

“…The cellulosome-producing, anaerobic mesophile Acetivibrio cellulolyticus, for example, is known both for its efficient degradation of crystalline cellulose and for its particularly elaborate cell surface ultrastructure (13,31,34,35,57). A novel scaffoldin (CipV) of this bacterium has recently been described and sequenced (15).…”

mentioning

confidence: 99%

The Cellulosome System of Acetivibrio cellulolyticus Includes a Novel Type of Adaptor Protein and a Cell Surface Anchoring Protein

Gao

Ding

et al. 2003

J Bacteriol

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“…The occurrence of cell-associated cellulolytic enzymes has been suggested in several anaerobic bacteria (6,9,10,21,24,32). It was the purpose of this work to determine whether our previous findings regarding the protuberant structures observed on the surface of C. thermocellum would be relevant to other cellulolytic bacteria.…”

mentioning

confidence: 88%

Specialized cell surface structures in cellulolytic bacteria

Lamed¹,

Naimark²,

Morgenstern³

et al. 1987

J Bacteriol

227

158

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The cell surface topology of various gram-negative and -positive, anaerobic and aerobic, mesophilic and thermophilic, cellulolytic and noncellulolytic bacteria was investigated by scanning electron microscopic visualization using cationized ferritin. Characterisitic protuberant structures were observed on cells of all cellulolytic strains. These structures appeared to be directly related to the previously described exocellular cellulase-containing polycellulosomes of Clostridium thermocellum YS

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“…Acetivibrio cellulolyticus. This bacterium was isolated from sewage sludge by Saddler and Khan (1979) and later described by Patel et al (1980) and Colvin et al (1982). It is an obligate anaerobe, and grows at pH 6.5-7.7 at temperatures from 20 to 40°C.…”

Section: Properties Of Some Anaerobic Cellulolytic Bacteriamentioning

confidence: 99%