The uptake of [3h] uridine into the nucleic acids of rat uterus during early pregnancy

Heald, P. J.; O'grady, J. E.

doi:10.1042/bj1140036pb

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…The increased [3H]-thymidine incorporation noted from day 6 onwards confirms that found previously (12). The latter authors also found a peak of incorporation of radioactivity from [3H]-uridine, presumably via [3 H]-cytidine, into DNA on day 6 of pregnancy.…”

Section: Discussionsupporting

confidence: 89%

Enzymic Activity in Rat Uterus during Early Pregnancy

Malinowska¹,

Fotherby²

1975

Horm Res

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Total protein, RNA and DNA content and the activity of acid and alkaline phosphatases, 5´-nucleotidase and isocitrate dehydrogenase were studied in rat uterus during the first 8 days of pregnancy. Isocitrate dehydrogenase activity showed marked fluctuations from day to day. Nucleotidase and acid phosphatase activities showed a significant increase on day 8. The most marked change in activity was that of alkaline phosphatase which showed a 10-fold increase between days 6 and 8, due largely to an increase in the activity of this enzyme in the decidual nodule. The rise in alkaline phosphatase activity did not occur in rats ovariectomized on days 1, 2 or 4 of pregnancy and was markedly decreased in those ovariectomized on day 6. [³H]-uridine incorporation into RNA showed a significant increase between days 2 and 6 whereas [³H]-thymidine incorporation into DNA showed a significant increase on day 6.

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Section: Discussionsupporting

confidence: 89%

Enzymic Activity in Rat Uterus during Early Pregnancy

Malinowska¹,

Fotherby²

1975

Horm Res

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“…The presence and roles of RBP in differentiation, particularly in decidualization, remains unexplored to date. Decidualization is a process with active protein synthesis (Heald & O'Grady, 1970), which implies active RNA regulation and processing. RBPs orchestrate transcript fate and function (Szostak & Gebauer, 2013).…”

Section: Discussionmentioning

confidence: 99%

CSDC2, a cold shock domain RNA‐binding protein in decidualization

Vallejo

Mestre-Citrinovitz

Winterhager³

et al. 2018

Journal Cellular Physiology

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RNA-binding proteins (RBPs) have been described for cancer cell progression and differentiation, although there is still much to learn about their mechanisms. Here, using in vivo decidualization as a model, we describe the role of RBP cold shock domain containing C2 (CSDC2) in the endometrium. Csdc2 messenger RNA expression was differentially regulated depending on time and areas of decidua development, with the most variation in antimesometrium (AM) and, to a lesser degree, in the junctional zone (JZ). Immunohistochemistry of CSDC2 showed a preferentially cytoplasmic localization at AM and JZ, and nuclear localization in underneath myometrium and mesometrium (M). Cytoplasmic localization coincided with differentiated, DESMIN-marked areas, while nuclear localization coincides with proliferative zones. Uterine suppression of CSDC2 through intrauterine-injected-specific small interfering RNA (siRNA) led to abnormal decidualization in early pregnancy, with more extended antimesometrial area and with poor M development if compared with control siRNA-injected animals. These results suggest that CSDC2 could be a regulator during decidua development.

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“…It has been reported that there are two periods of rapid RNA biosynthesis in the uterus during early pregnancy (Heald and O’Grady, 1970), one on the 2 nd day after pregnancy and the other on the 4 th day. The second increase in RNA synthesis coincides with the beginning of the decidual differentiation of endometrial stromal cells.…”

Section: Discussionmentioning

confidence: 99%

Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

Vallejo

Maschi

Mestre-Citrinovitz

et al. 2009

Journal Cellular Physiology

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During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3 fold, FDR > 0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.

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The uptake of [3h] uridine into the nucleic acids of rat uterus during early pregnancy

Cited by 9 publications

References 4 publications

Enzymic Activity in Rat Uterus during Early Pregnancy

Enzymic Activity in Rat Uterus during Early Pregnancy

CSDC2, a cold shock domain RNA‐binding protein in decidualization

Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

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