The uptake of alpha-foetoprotein by C-1300 mouse neuroblastoma cells

Hajeri-Germond, M.; Naval, Javier; Trojan, Jerzy; Uriel, J

doi:10.1038/bjc.1985.123

Cited by 26 publications

(24 citation statements)

References 15 publications

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“…Comparative studies of different antigens such as AFP, IGF-I, IGF-II, VEGF, and EGF present in neoplastic cells [6,7,28] have demonstrated that IGF-I constitutes an important target for genetic testing and therapeutic purpose. The arrest of IGF-I expression diminishes or stops neoplastic development [7,29,30] due to the consequential apoptosis and anti-tumor immune response (MHC-I) [31][32][33] .…”

Section: Igf-1 Biomarkermentioning

confidence: 99%

IGF-I biomarker testing in an ethical context

Trojan¹,

Aristizábal

Jay³

et al. 2016

Adv Mod Oncol Res

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Abstract:As we have come to know, there is a connection between cancer biomarkers and genes, along with their susceptibility to a particular disease, all of which have an obvious impact on the clinical practice and development of genetic testing. In any cancer disease, the diagnosis and treatment should be related to the investigation of specific biomarkers (generally antigens and proteins) and their corresponding genes. The study of different antigens such as alpha-fetoprotein, insulin-like growth factor I (IGF-I), insulin-like growth factor II, vascular endothelial growth factor, and epidermal growth factor, as well as their presence in neoplastic cells have demonstrated that IGF-I is an essential target for gene testing and therapeutic purpose. An over-expression of the IGF-I gene in mature tissues is a sign of neoplastic processes, e.g. brain or breast malignancy. A lot of questions have arisen regarding the ethics of gene testing, particularly concerns on the selection of patients for specific growth hormone/insulin-like growth factor I (GHIIGF-I) testing. Evidently, our current society is involved in a process of geneticization -the redefinition of individuals in terms of genetic codes. As such, we should take extreme care when making ethical judgments based on "scientific evidence" derived from genetic testing (typically those involving different biomarkers such as DNA, RNA, chromosomes, and proteins) in relation to genetic abnormalities that could predict current or future diseases. In this situation, the understanding of bioethics is of utmost importance.

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Section: Igf-1 Biomarkermentioning

confidence: 99%

IGF-I biomarker testing in an ethical context

Trojan¹,

Aristizábal

Jay³

et al. 2016

Adv Mod Oncol Res

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“…To investigate the expression of the AFP gene in cultured cells, we adapted previously described techniques for in situ hybridization of mRNAs in solid tissues [41] and for immunoperoxi dase labelling of AFP [12,42]. The uptake of AFP and its FITC conjugates by S and F cells was studied by immunocytochemical [15] and immunofluorometric tech niques [43].…”

Section: A-fetoprotein Schwann Cells Fibroblasts Immunocytochemistry mentioning

confidence: 99%

“…After a 48-hour incubation, the medium was removed and the plates incubated for 2-3 h in serum-free medium to deplete cells of endogenous bovine AFP. Fresh medium containing 100 pg/ml of the following purified native proteins or their FITC conjugates [16] were then added (1 ml/plate): mouse or rat AFP [13]; mouse or rat SA as positive control [14,15], or ovalbumin and lysine as negative controls [15,16]. Moreover, FITC conjugates of bovine AFP and SA were tested.…”

Section: Uptake O F Afp By Cultured Cellsmentioning

confidence: 99%

Demonstration of Segmental Arrangement of Thoracic Spinal Motor Neurons Using Lipophilic Dyes

Tsering

1992

Dev Neurosci

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Unlike the afferent input into the spinal cord, it is generally believed that the motor efferent system in the mammalian spinal cord is more segmental in arrangement. Isolated comments in recent reports suggest that motor neurons in the mammalian spinal cord may be strictly segmental, contrary to many earlier reports using less sophisticated methods. We have attempted to address the question of the segmental arrangement of motor neurons in a mammalian spinal cord using single and double labelling with the fluorescent lipophilic dyes, Di I and Di A. These dyes have the ability to diffuse along and stain the plasma membrane of the neural elements in aldehyde-fixed specimens. Sprague-Dawley rat fetuses and early postnatal pups were used. The study was conducted on the thoracic spinal cord. The results confirm that thoracic spinal motor neurons are restricted to the segments from which their axons exit via the ventral roots in the intrauterine stages of development. The segmental arrangement of motor neurons in the thoracic spinal cord corresponded well with that of the sympathetic preganglionic neurons which were stained simultaneously.

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“…In vitro and in vivo studies showed that malignant cells regain the ability to take up afp via a receptor that would be present in undifferentiated cells of either embryonic or tumour origin [10][11][12][13][14][15] , but mostly absent in normal adult cells. The existence of such a receptor for afp (recaf) was then demonstrated and functionally characterized in several cell lines [16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Increased Alpha-Fetoprotein Receptor in the Serum of Patients with Early-Stage Breast Cancer

Moro¹,

Tcherkassova²,

Stieber³

2012

Current Oncology

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The alpha-fetoprotein (afp) receptor (recaf) is an oncofetal antigen found in most types of cancer. Using a competitive radioimmunoassay, we measured the concentration of serum recaf in three sets of samples. Set 1 was blind and consisted of 119 normal subjects, 43 breast cancer patients (stages i and ii), and 20 patients with benign breast conditions. In this set, the assay discriminated normal from cancer samples with a receiver operating characteristic for the area under the curve (ROCAUC) of 0.983; with 95% specificity and 93% sensitivity at a cut-off of 4.6 K (arbitrary) recaf units; and with 72% sensitivity and 100% specificity at a cut-off of 7.3 K units. At 7.3 K units, the specificity for benign breast conditions was 85%, and the sensitivity was 72% (ROCAUC was 0.773). Carcinoembryonic antigen and cancer antigen 15-3 respectively showed 39% and 41% sensitivity, with 95% specificity in comparisons of normal with cancer samples, and 34% and 44% sensitivity, with 85% specificity in comparisons of benign with cancer samples. Set 2 consisted of 353 normal, 30 benign, and 64 cancer samples (stages ii and iii). The recaf assay sensitivity in discriminating normal from cancer samples was 97%, with 97% specificity. Benign compared with cancer samples showed 87% sensitivity, with 97% specificity. Set 3 included only 40 normal and 40 cancer samples. The assay sensitivity was 89%, with 100% specificity. Sets 2 and 3 were not tested with carcinoembryonic antigen and cancer antigen 15-3. These results strongly suggest that the recaf assay could be used for detecting breast cancer in its early stages.

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The uptake of alpha-foetoprotein by C-1300 mouse neuroblastoma cells

Cited by 26 publications

References 15 publications

IGF-I biomarker testing in an ethical context

IGF-I biomarker testing in an ethical context

Demonstration of Segmental Arrangement of Thoracic Spinal Motor Neurons Using Lipophilic Dyes

Increased Alpha-Fetoprotein Receptor in the Serum of Patients with Early-Stage Breast Cancer

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