The uptake of foreign ferritin by macrophages in the spleen, trunk kidney and liver of platy

Leknes, Ingvar Leiv

doi:10.1111/j.1095-8649.2001.tb00203.x

Cited by 15 publications

(11 citation statements)

References 16 publications

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“…In this study, we demonstrated that more than 90% of the iron, injected as IDx, into the peritoneal cavity of the sea bass (Dicentrarchus labrax L.), was absorbed within 6 h. This is similar to what has been observed in rats (Suzuki et al 1995) and in other fish species (Leknes 2001(Leknes , 2002). …”

Section: Discussionsupporting

confidence: 74%

“…In addition, this organ showed a similar histological structure to the spleen of platy and sea bream, where MMCs are widely distributed over the whole organ and normally contain large amounts of iron (Manera et al 2000, Leknes 2001. After IDx i.p.…”

Section: Discussionmentioning

confidence: 95%

“…The presence of MMCs or macrophage aggregates (MA) has been reported in the liver of some teleost fishes (Manera et al 2000, Leknes 2001). However, these structures were not observed in sea bass.…”

Section: Discussionmentioning

confidence: 99%

“…Although the head kidney is known to be predominantly a lymphoid organ (Press & Evensen 1999), there are some similarities with the spleen structures, namely the presence of MMCs, which also accumulate iron (Manera et al 2000, Leknes 2001). There is a lack of research on iron concentration in the fish head kidney.…”

Section: Discussionmentioning

confidence: 99%

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A model for acute iron overload in sea bass (Dicentrarchus labrax L.)

Rodrigues

Pereira

2004

Lab Anim

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SummaryEvaluation of several parameters involved in iron metabolism was carried out after intraperitoneal (i.p.) injection with iron dextran (IDx) in sea bass (Dicentrarchus labrax L.). After treatment, a rapid mobilization of IDx from the peritoneal cavity to other organs was observed. This was followed by a modification of normal peripheral blood iron parameters. Total iron (TI) and transferrin saturation (TS) rose rapidly, to 4.14 g/ml and 83.7%, respectively, on day 3. In contrast, unsaturated iron binding capacity (UIBC) dropped from 3.19 g/ml (at day 0) to 0.90 g/ml on day 3. Tissue iron content was determined by atomic absorption spectometry (AAS). Three days post-IDx injection, values of iron concentration in liver, spleen and head kidney were significantly higher than control values (15, 6 and 9-fold increase, respectively). Samples of liver, spleen and head kidney were processed for routine histology, and the Perl's method was used for iron staining. Histological sections of the IDx-treated animals showed iron deposition in all tissues studied. In the liver, the iron was evenly distributed over the whole organ, being present in the hepatocytes. In the head kidney and spleen, the iron deposition was mainly observed in the melanomacrophage centres (MMCs). The present study characterizes several parameters involved in iron metabolism, and develops a fish model, of iron overload, which can be used in further studies of iron toxicity and iron-induced susceptibility to bacterial infections.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A model for acute iron overload in sea bass (Dicentrarchus labrax L.)

Rodrigues

Pereira

2004

Lab Anim

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“…Recent studies have revealed that the specialized endocardium in teleosts is able to take up large amounts of various foreign organic macromolecules from the blood stream (Leknes 1987, Smedsrød et al 1995, Dalmo et al 1996, Sørensen et al 1998, Leknes 2001a. The macrophages in the trunk kidney and spleen of platy Xiphophorus maculatus are able to internalize foreign proteins (Leknes 2001b).…”

mentioning

confidence: 99%