The Urinary Excretion of Riboflavin Fluorometric Methods for Its Estimation

Ferrebee, Joseph W.

doi:10.1172/jci101118

Cited by 57 publications

(9 citation statements)

References 12 publications

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“…Some idea of the degree of deficiency may be obtained by following the daily excretion of riboflavin for several consecutive days. These results are in agreement with those of Ferrebee (15).…”

Section: Methodssupporting

confidence: 93%

A Study of Urinary Riboflavin Excretion in Man 1

Axelrod¹,

Spies²,

Elvehjem³

et al. 1941

J. Clin. Invest.

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The occurrence of riboflavin deficiency in man has been described by several investigators (1 to 5). Since urinary excretion tests have proved to be of considerable value in the diagnosis of thiamin and ascorbic acid deficiencies, the present study was undertaken to determine the value of such tests in the diagnosis of riboflavin deficiency.A pronounced decrease in the urinary riboflavin excretion has been observed in rats and dogs maintained on riboflavin-low diets (6,7,8). Spies, Bean and Ashe (9) have reported a decrease in the amount of riboflavin present in the urine of pellagrins with riboflavin deficiency, and Emmerie (10) has observed that urinary riboflavin excretion of a subject on a diet restricted in riboflavin was 43 to 60 per cent of that observed when the subject was on a normal diet. Both groups of investigators (9, 10) noted that the administration of riboflavin was followed by a rapid excretion of the vitamin in the urine. Neuweiler (11) reported a case of ariboflavinosis in an infant who completely retained large doses of riboflavin. MATERIAL AND METHODSFive patients in the Nutrition Clinic of the Hillman Hospital, Birmingham, Alabama, were chosen for this study. This group (Cases I, II, III, IV and V) consisted of malnourished persons who were suffering from a multiple vitamin deficiency. They were hospitalized throughout the study and were restricted to diets deficient Twenty-four-hour urine specimens were collected from each patient in dark bottles containing 1 cc. of glacial acetic acid and a small amount of toluene. The volume was measured and aliquots were brought to pH 6.6 and stored in the refrigerator under toluene. Samples were collected over a period of two to four weeks.The riboflavin content of the urine was determined by the microbiological method of Snell and Strong (12). The reliability of this method for the determination of both the free and combined forms of riboflavin in a number of biological fluids has been adequately established (12, 13). When necessary, the urine was diluted with distilled water in order to adjust the riboflavin content to the correct assay range (approximately 0.1 microgram of riboflavin per ml.). All assays were made at three different levels, each level being run in duplicate. It was found that the sample of urine could be stored in the refrigerator for a period of two weeks with no loss in the riboflavin content.The "saturation" tests were performed by administering riboflavin through intravenous injection and determining the riboflavin content of the following twenty-

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Section: Methodssupporting

confidence: 93%

A Study of Urinary Riboflavin Excretion in Man 1

Axelrod¹,

Spies²,

Elvehjem³

et al. 1941

J. Clin. Invest.

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“…In the present investigation no lutein (absorption maxima 445, 475 and 508 mp in carbon disulphide- Kuhn & Spaakula, 1931;Zubrys & Morf, 1933) was found. Strain (1938, 1941 reported that when lutein was boiled in methanolic solution for four hours or treated with iodine solution, two isomers, one having absorption maxima at 447, 474 and 505 mr/ in carbon disulphide and the other at 469 and 497 m,u were formed. The absorption maxima of our xanthophyll pigments 'X' and 'Y' are close to these.…”

Section: Discussionmentioning

confidence: 99%

Carotenoid pigments of Badami mango fruit

Ramasarma

Rao

Hakim

1946

Biochemical Journal

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“…A modification of Ferrebee's (1940) method was used for riboflavin analysis. The fluorescence produced after treatment with potassium permanganate and hydrogen peroxide was also read in the photofluorometer.…”

Section: Assay Methodsmentioning

confidence: 99%

Stability of Added Vitamins in Beer

Younger¹,

Harvey

1945

Journal of Food Science

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The stability of vitamins in solution depends upon several factors, such as temperature, pH, oxidation, and exposure to light. Appreciable quantities of several B-complex vitamins, with the exception of thiamin. have been found in fermented malt beverages by Laufer, Schwarz, and Laufer (1942 1. The possibility of increasing the vitamin content by improved malting and brewing methods, makes it desirable to know the effect of varying conditions of storage on the stability of water-soluble vitamins in beer.Novak and Adams (1943) added definite amounts of thiamin, riboflavin, and niacin to separate portions of 86-proof whiskey in both clear and amber bottles and exposed them to daylight. They found no loss of thiamin or niacin in either bottle, but 50 per cent of the riboflavin was destroyed in the clear-glass bottle a t the end of the first week, and within two months the amber bottle and Control bottle, kept in the dark, had both lost 50 per cent of their riboflavin.Evidently the action of light was primarily responsible for the rapid loss in the clear bottle, while less obvious factors must be taken into account to explain the change in the other bottles. I n either case, the destruction of riboflavin was probably not accelerated by any chemical change in the whiskey itself. The high alcoholic content and hinute amount of residual matter account for its stability.Beer, a beverage of low alcoholic content, contains complex extractives including carbohydrates and protein derivatives. These are subject to chemical change if the bottled beer is stored under unfavorable conditions such as were chosen for part of this work. This was done to gain a comparison of the stability of certain vitamins in solution under normal and abnormal storage conditions. EXPERIMENTAL PROCEDUREThe natural vitamin content of beer was supplemented with thiamin, riboflavin, and niacin. These vitamins are important in human nutrition and are quite stable in acid solution when subjected to a pasteurizing temperature of 60°C.(1400F.).Measured amounts of beer of approximately four per cent alcoholic content b y weight were drawn into three stainless-steel quarter barrels from a large storage tank. The first barrel was fortified with thiamin on the basis of 0.425 mg. per 12 fluid ounces (355 ml.), this being the liquid content of a standard beer bottle. To the second barrel riboflavin was added in the amount of 0.915 mg. per 12 fl. oz., and the third barrel received niacin on the basis of 19.5 mg. per 12 fl. oz. Each vitamin had

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The Urinary Excretion of Riboflavin Fluorometric Methods for Its Estimation

Cited by 57 publications

References 12 publications

A Study of Urinary Riboflavin Excretion in Man 1

A Study of Urinary Riboflavin Excretion in Man 1

Carotenoid pigments of Badami mango fruit

Stability of Added Vitamins in Beer

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