Carotenoid pigments of Badami mango fruit

Ramasarma, G. B.; Rao, S. D.; Hakim, D. N.

doi:10.1042/bj0400657

Cited by 6 publications

(2 citation statements)

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“…This copurification of these two inhibitory activities indicated that both the activities may reside on the same protein. Such double-headed inhibitors have been reported from horsegram (Ramasarma and Rajagopal Rao, 1991) and most of the plants which have Bowman-Birk inhibitors (Laskowski and Kato, 1980).…”

Section: Purificationmentioning

confidence: 84%

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Purification and Characterization of a Proteinase Inhibitor from Field Bean, Dolichos lablab perpureus L.

Devaraj

Manjunatha

1999

J Protein Chem

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A proteinase inhibitor resembling Bowman-Birk family inhibitors has been purified from the seeds of cultivar HA-3 of Dolichos lablab perpureus L. The protein was apparently homogeneous as judged by SDS-PAGE, PAGE, IEF, and immunodiffusion. The inhibitor had 12 mole% 1/2-cystine and a few aromatic amino acids, and lacks tryptophan. Field bean proteinase inhibitor (FBPI) exhibited a pl of 4.3 and an M r of 18,500 Da. CD spectral studies showed random coiled secondary structure. Conformational changes were detected in the FBPI-trypsin/chymotrypsin complexes by difference spectral studies. Apparent K a values of complexes of inhibitor with trypsin and chymotrypsin were 2.1 X 10 7 M -1 and 3.1 X 10 7 M -1 , respectively. The binary and ternary complexes of FBPI with trypsin and chymotrypsin have been isolated indicating 1:1 stoichiometry with independent sites for cognate enzymes. Amino acid modification studies showed lysine and tyrosine at the reactive sites of FBPI for trypsin and chymotrypsin, respectively.

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Section: Purificationmentioning

confidence: 84%

“…of lima bean (Norton, 1991), ground nut (Ikenaka and Norioka, 1986), and horsegram (Ramasarma and Rajagopal Rao, 1991), the FBPI is also devoid of Trp. The absence of free thiol groups in FBPI despite a 12 mol % cysteine suggests the existence of disulfide bridges as observed in many BBIs.…”

Section: Specificitymentioning

confidence: 99%