The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis

Cave, Eleanor; Crowther, Nigel J.

doi:10.1007/978-1-4939-8994-2_25

Cited by 25 publications

(28 citation statements)

References 9 publications

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“…Beside the methodological procedure, it is relevant the choice of the cell line. 3T3-L1 adipocytes and SGBS pre-adipocytes are widespread used in vitro models for providing detailed insights into the molecular mechanisms governing adipogenesis (Capel et al, 2012; Zha et al, 2013; Ahonen et al, 2019; Cave and Crowther, 2019). Although 3T3-L1 adipocytes have some limitations and could not be completely representatives of in vivo conditions, they possess some advantages: are homogenous in terms of cellular population and their cells are all at the same differentiation stage, giving a homogeneous response to treatments.…”

Section: Discussionmentioning

confidence: 99%

miRNA-218 Targets Lipin-1 and Glucose Transporter Type 4 Genes in 3T3-L1 Cells Treated With Lopinavir/Ritonavir

et al. 2019

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Background: Metabolic complications represent a common and serious problem associated with HIV infection and combined Antiretroviral Therapy (cART). Alterations in body fat distribution are associated with significantly increased risks of (i) metabolic derangements, (ii) cardiovascular pathologies, and (iii) insulin resistance. A case control study showed that in subcutaneous adipose tissue from HIV-infected patients on cART presenting lipodystrophy (LS), the levels of miRNA-218 were upregulated and those of lipin-1, a putative target gene of miRNA-218, were downregulated compared with HIV-negative subjects. Lipin-1 is one of the most important factors linked to development of LS. Lipin-1, by controlling PPARγ2, regulates the expression of specific genes, such as that of glucose transporter type 4 (GLUT-4), required for maturation and maintenance of adipocytes. Objectives: To determine whether lopinavir/ritonavir (LPV/RTV) can modulate lipogenesis in adipocytes affecting miRNA-218 and lipin-1 mRNA expression, and to investigate the functional link between miRNA-218 and GLUT-4 mRNA expression. Methods: Differentiated 3T3-L1 cells were treated with various combinations of LPV/RTV, followed by measurements of cell viability, lipid accumulation, lipin-1 and GLUT-4 mRNA and miRNA-218 levels. Transfection of anti-miR-218 or a miRNA-218 mimic were used to investigate the role of miRNA-218 in lipogenesis. Results: LPV/RTV treatment of 3T3-L1 cells did not affect the viability of differentiated 3T3-L1 cells, but caused (i) a significant decrease of lipid accumulation, (ii) an overexpression of miRNA-218, and (iii) a reduction of lipin-1 and GLUT-4 mRNA levels. The anti-miR-218 transfection of 3T3-L1 cells significantly ameliorated the adipogenic dysfunction and restored mRNA levels of lipin-1 and GLUT-4 consequent to LPV/RTV treatment. By contrast, 3T3-L1 cells transfected with a specific miRNA-218 mimic showed (i) an overexpression of miRNA-218, (ii) a reduced cellular lipid fraction, and (iii) decreased levels of mRNA for lipin-1 and GLUT-4. Conclusion: 3T3-L1 cells, treated with LPV/RTV, show altered lipid content due to increased miRNA-218 levels, which affects lipin-1 mRNA. Moreover, increased miRNA-218 levels were inversely correlated with changes in GLUT-4 expression, which suggests a role for miRNA-218 in mediating the insulin resistance consequent to cART.

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Section: Discussionmentioning

confidence: 99%

miRNA-218 Targets Lipin-1 and Glucose Transporter Type 4 Genes in 3T3-L1 Cells Treated With Lopinavir/Ritonavir

et al. 2019

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“…A genome-wide association study revealed that the singlenucleotide polymorphisms in FTO region are correlated with body mass in multiple populations (20,21). Intriguingly, FTO is the first demethylase identified to remove the N 6 methyladenosine (m 6 A) on messenger RNA (mRNA) (22,23). A number of studies have indicated that FTO plays certain role in the regulation of adipocyte generation via targeting m 6 A-containing regulatory mRNAs (24)(25)(26)(27)(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

“…Intriguingly, FTO is the first demethylase identified to remove the N 6 methyladenosine (m 6 A) on messenger RNA (mRNA) (22,23). A number of studies have indicated that FTO plays certain role in the regulation of adipocyte generation via targeting m 6 A-containing regulatory mRNAs (24)(25)(26)(27)(28)(29)(30)(31). For example, FTO could mediate exonic splicing of adipogenic regulatory factor RUNX1T1 through adjusting m 6 A abundance around splice sites and, thus, regulating differentiation (26).…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies have indicated that FTO plays certain role in the regulation of adipocyte generation via targeting m 6 A-containing regulatory mRNAs (24)(25)(26)(27)(28)(29)(30)(31). For example, FTO could mediate exonic splicing of adipogenic regulatory factor RUNX1T1 through adjusting m 6 A abundance around splice sites and, thus, regulating differentiation (26). Whether rhein could rearrange global m 6 A methylome landscape, and whether FTO-mediated demethylation participates in rhein-interfered adipocyte differentiation remain elusive…”

Section: Introductionmentioning

confidence: 99%

“…For example, FTO could mediate exonic splicing of adipogenic regulatory factor RUNX1T1 through adjusting m 6 A abundance around splice sites and, thus, regulating differentiation (26). Whether rhein could rearrange global m 6 A methylome landscape, and whether FTO-mediated demethylation participates in rhein-interfered adipocyte differentiation remain elusive…”

Section: Introductionmentioning

confidence: 99%

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The Phytochemical Rhein Mediates M6A-Independent Suppression of Adipocyte Differentiation

et al. 2021

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Adipogenesis is mediated by the complex gene expression networks involving the posttranscriptional modifications. The natural compound rhein has been linked to the regulation of adipogenesis, but the underlying regulatory mechanisms remain elusive. Herein, we systematically analyzed the effects of rhein on adipogenesis at both the transcriptional and posttranscriptional levels. Rhein remarkably suppresses adipogenesis in the stage-specific and dose-dependent manners. Rhein has been identified to inhibit fat mass and obesity-associated (FTO) demethylase activity. Surprisingly, side-by-side comparison analysis revealed that the rhein treatment and Fto knockdown triggered the differential gene regulatory patterns, resulting in impaired adipocyte formation. Specifically, rhein treatment mildly altered the transcriptome with hundreds of genes dysregulated. N6-methyladenosine (m6A) methylome profile showed that, although the supply of rhein induced increased m6A levels on a small subset of messenger RNAs (mRNAs), few of them showed dramatic transcriptional response to this compound. Moreover, the specific rhein-responsive mRNAs, which are linked to mitotic pathway, are barely methylated or contain m6A peaks without dramatic response to rhein, suggesting separate regulation of global m6A pattern and adipogenesis mediated by rhein. Further identification of m6A-independent pathways revealed a positive regulator, receptor expressing-enhancing protein 3 (REEP3), in guidance of adipogenesis. Hence, this study provides the mechanistic view of the cellular actions of rhein in the modulation of adipogenesis and identifies a potential novel target for obesity therapeutic research.

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Different types of bile acids exhibit opposite regulatory effects on lipid metabolism in finishing pigs through bile acid receptors

Hu,

Sang,

et al. 2024

Animal Nutrition

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The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis

Cited by 25 publications

References 9 publications

miRNA-218 Targets Lipin-1 and Glucose Transporter Type 4 Genes in 3T3-L1 Cells Treated With Lopinavir/Ritonavir

miRNA-218 Targets Lipin-1 and Glucose Transporter Type 4 Genes in 3T3-L1 Cells Treated With Lopinavir/Ritonavir

The Phytochemical Rhein Mediates M6A-Independent Suppression of Adipocyte Differentiation

Different types of bile acids exhibit opposite regulatory effects on lipid metabolism in finishing pigs through bile acid receptors

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