The Use of a Portable Tissue Culture Laboratory in a Field Study of Tropical Poliomyelitis

Epidemic of Poliomyelitis in Puerto Rico, 1960

“…From these calculations, the estimate of vaccine effectiveness in San Juan for three or more doses of Salk vaccine was 82 percent. This also agrees with the findings of Melnick during the first epidemic of poliomyelitis in British Guiana, which occurred in 1957 (19). Puerto Rico.…”

Miniature Cell Culture Systems for Use in Field Isolation of Viruses: Cell Stability and Sensitivity to Selected Laboratory-Propagated Viruses During Storage in Miniaturized Environments 1

“…The morphological stability of HEL cells treated similarly was nearly as good, and even though HEK, WI-38, and MK cells were not as stable in this respect, they still exhibited fair morphology after 1 month of storage. HeLa cells, however, deteriorated rather rapidly under these conditions; within 1 week some detachment had already occurred.…”

“…Each tube contained an essentially confluent cell monolayer exhibiting normal gross morphology at the beginning of storage. Within 2 weeks all were severely degenerated; however, the human embryonic cells did retain good to excellent morphology for approximately 1 week under these conditions. Additional work gave results which indicated that cells degenerated most rapidly when both ends of the capillary were sealed with putty and least rapidly when the one open end was kept under water to prevent internal evaporation.…”