The use of AFLP to find an informative SNP: genetic differences across a migratory divide in willow warblers

Bensch, Staffan; Åkesson, Susanne; Irwin, Darren E.

doi:10.1046/j.1365-294x.2002.01629.x

Cited by 112 publications

(112 citation statements)

References 32 publications

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“…Similar patterns have been found in many studies of other animals (for example, bears: Taberlet et al (1995); willow warblers: Bensch et al (2002); shrews : Andersson,(2004)). The proposed explanation for this north-south divide is the post-glacial reinvasion of the Scandinavian Peninsula following the retreat of the inland ice approximately 10 000 years ago.…”

Section: Discussionsupporting

confidence: 86%

Inference of hazel grouse population structure using multilocus data: a landscape genetic approach

2008

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In conservation and management of species it is important to make inferences about gene flow, dispersal and population structure. In this study, we used 613 georeferenced tissue samples from hazel grouse (Bonasa bonasia) where each individual was genotyped at 12 microsatellite loci to make inference on population genetic structure, gene flow and dispersal in northern Sweden. Observed levels of genetic diversity suggest that Swedish hazel grouse do not suffer loss of genetic diversity compared with other grouse species. We found significant F IS (deviation from Hardy-Weinberg expectations) over the entire sample using jack-knifed estimators over loci, which is most likely explained by a Wahlund effect. With the use of spatial autocorrelation methods, we detected significant isolation by distance among individuals. Neighbourhood size was estimated in the order of 62-158 individuals corresponding to a dispersal distance of 950-1500 m. Using a spatial statistical model for landscape genetics to infer the number of populations and the spatial location of genetic discontinuities between these populations we found indications that Swedish hazel grouse are divided into a northern and a southern population. We could not find a sharp border between these two populations and none of the observed borders appeared to coincide with any potential geographical barriers.These results imply that gene flow appears somewhat unrestricted in the boreal taiga forests of northern Sweden and that the two populations of hazel grouse in Sweden may be explained by the post-glacial reinvasion history of the Scandinavian Peninsula.

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Section: Discussionsupporting

confidence: 86%

Inference of hazel grouse population structure using multilocus data: a landscape genetic approach

2008

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“…Marker polymorphism was calculated using GenAlEx (Peakall and Smouse 2012) and is presented in Table 1. Genotyping of the WW1 and WW2 bi-allelic markers followed previously published methods (Bensch et al 2002(Bensch et al , 2009). Some markers did not amplify for a small number of individuals; see results tables for specifi c numbers.…”

Section: Genetic Analysesmentioning

confidence: 99%

“…Further, geographic variation is also demonstrated at the WW1 locus (Bensch et al 2002, Lundberg et al 2011. Bensch et al (2002) suggested that this is a noncoding SNP, which Lundberg et al (2011) located in a genomic region of about 2.5 Mb that is diff erentiated between northern and southern willow warblers in Sweden. As the geographic pattern of the WW1 marker is more consistent with habitat and climatic factors than migration routes, selective forces related to environmental variables might act upon genomic regions that are linked to the WW1 locus (Lundberg et al 2011).…”

mentioning

confidence: 98%

“…Th ey also spend the winter in different regions of sub-Saharan Africa (Chamberlain et al 2000). It is thought that the two migratypes refl ect the colonization of Scandinavia from two directions after the last ice age; trochilus from the south and acredula from the north (Bensch et al 1999(Bensch et al , 2002(Bensch et al , 2009. Th e migratypes diff er genetically at the bi-allelic WW2 locus, which might be linked to genes associated with migratory behaviour (Bensch et al 2009).…”

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confidence: 99%

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Weak geographical structure in sperm morphology across the range of two willow warbler Phylloscopus trochilus subspecies in Scandinavia

Støstad

Rekdal

Kleven

et al. 2016

Journal of Avian Biology

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Sperm morphology is highly diversified among species and at higher taxonomic levels. In birds, there is also increasing evidence of geographical differentiation in sperm traits within species, especially in those with strong sperm competition. Geographical divergences in sperm traits might imply the formation of a reproductive barrier in a speciation process. Here we study sperm morphology variation of willow warblers Phylloscopus trochilus in a geographical context in Scandinavia, across the range of two subspecies that are differentiated in certain genetic markers, morphology and migratory routes. We describe geographical patterns in genotypes (two previously described single‐nucleotide polymorphism (SNP) markers and four polymorphic microsatellites); in wing length, tarsus length and body mass; and in sperm traits of 330 male willow warblers sampled at 33 localities across Norway (58°N–69°N). Birds were on average larger and longer‐winged in the north (spp. acredula) than in the south (spp. trochilus), and showed a sigmoid change in the SNP allele frequencies and body morphology around 65°N. We found no evidence of genetic structuring in the microsatellites. There was no geographical variation in sperm traits across Norway, except that sperm heads were on average longer in the south. Sperm head length was also associated with the two SNP markers, with longer sperm heads for the southern alleles, and midpiece length was weakly associated with one of the SNP markers. Similar among‐male variances in total sperm length among the 33 sampling sites indicate uniform levels of sperm competition across Norway. We conclude that sperm morphology remains a rather undifferentiated trait between the two willow warbler subspecies in Scandinavia, which is consistent with a pattern of a shallow genetic divergence. This indicates that sperm morphology is not a reproductive barrier maintaining the narrow hybrid zone.

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“…Reports on potential problems encountered during the selection of molecular markers are also im- 686 Faria et al portant to improve general understanding of their dynamics in different groups. For future studies involving population genetics of terns, the utilization of other kinds of molecular markers that have proved to be suitable to conduct genetic studies in other species of birds might be considered, such as nuclear introns (Congdon et al, 2000;Pacheco et al, 2002;Friesen et al, 2005;Whittier et al, 2006), SNPs (Saetre et al, 2001;Bensch et al, 2002) and AFLP (Wang et al, 2003;Boulet et al, 2005;Helbig et al, 2005).…”

Section: Abbreviations: Sh (S Hirundinacea) Tm (T Maximus) Sf (Smentioning

confidence: 99%

Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves)

et al. 2007

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Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control region, ND2, Cytochrome b and ATPase 6/8) for phylogeographic studies in this group of birds. The occurrence of nuclear copies of the mitochondrial control region is reported. Nucleotide divergence in the mtDNA genes analyzed ranged from 0.0 to 0.006. Despite the difficulties associated with the selection of variable markers in this group of seabirds, we were able to select polymorphic markers for each species tested and we anticipate these results will help the development of genetic studies concerning important biological questions in terns.

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The use of AFLP to find an informative SNP: genetic differences across a migratory divide in willow warblers

Cited by 112 publications

References 32 publications

Inference of hazel grouse population structure using multilocus data: a landscape genetic approach

Inference of hazel grouse population structure using multilocus data: a landscape genetic approach

Weak geographical structure in sperm morphology across the range of two willow warbler Phylloscopus trochilus subspecies in Scandinavia

Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves)

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