The use of Aloe vera gel coating supplemented with Pichia guilliermondii BCC5389 for enhancement of defense-related gene expression and secondary metabolism in mandarins to prevent postharvest losses from green mold rot

Jiwanit, Palakrit; Pitakpornpreecha, Thanawat; Pisuchpen, Supachai; Leelasuphakul, Wichitra

doi:10.1016/j.biocontrol.2017.08.023

Cited by 28 publications

(10 citation statements)

References 43 publications

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“…A recent report has shown a similar result where thyme essential oil reduced anthracnose disease incidence via up-regulated expression of the PAL gene and down-regulated expression of the LOX gene in ‘Hass’ and ‘Ryan’ avocado fruit [42]. The defense mechanism of postharvest CEO treatment exhibited in the present work is in agreement with the previous reports regarding the gene expressions of defense-related enzymes in cyclic lipopeptides-treated ’Valencia’ orange fruit [44] and Aloe vera gel-treated ’Shogun’ mandarins [43], as well as in thyme and savory essential oil treated apples [22] subjected to fungal infections.…”

Section: Resultssupporting

confidence: 91%

“…Tea tree oil vapor treatment significantly enhanced the disease resistance of postharvest strawberry fruit against Botrytis cinerea through the induction of five defense-related enzymes, such as PAL, POD, PPO, β-Glu and CHI [24]. Likewise, Aloe vera gel treatment induced the activities of defense-related enzymes, including LOX, PAL and POD in ’Shogun’ mandarins inoculated with P. digitatum , the causal agent of postharvest green mold in citrus fruit [43]. Thus, CEO treatment may induce citrus fruit resistance against P. italicum through a pathogen-dependent priming mechanism.…”

Section: Resultsmentioning

confidence: 99%

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Clove Essential Oil as an Alternative Approach to Control Postharvest Blue Mold Caused by Penicillium italicum in Citrus Fruit

et al. 2019

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Penicillium italicum causes blue mold disease and leads to huge economic losses in citrus production. As a natural antifungal agent, clove essential oil (CEO), which is a generally recognized as safe (GRAS) substance, shows strong in vitro activity against fungal pathogens. However, few studies on CEO for controlling postharvest blue mold disease caused by P. italicum in citrus fruit have been reported. Our aims were to investigate the control efficacy and possible mechanisms involved of CEO against P. italicum. In the present study, CEO treatment inhibited the disease development of blue mold when applied at 0.05% to 0.8% (v/v), and with the effective concentration being obtained as 0.4% (v/v). Besides its direct antifungal activity, CEO treatment also spurred a rapid accumulation of H2O2 compared with untreated fruits, which might contribute to enhancing an increase in the activities of defense-related enzymes, such as β-1,3-glucanase (β-Glu), chitinase (CHI), phenylalanine ammonia-lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO), and lipoxygenase (LOX) in citrus fruit. Results of real time-quantitative polymerase chain reaction (RT-qPCR) showed that the gene expressions of β-Glu, CHI, PAL, POD and PPO were up-regulated in CEO-treated fruits. At the same time, CEO treatment led to down-regulated expression of the LOX gene in citrus fruit. Clove essential oil effectively control the disease incidence of blue mold decay in citrus fruit by motivating the host-defense responses, suppressing the malondialdehyde (MDA) accumulation while enhancing the activities and gene expressions of defense-related enzymes. Our study provides an alternative preservative applying CEO to reduce postharvest fungal decay in citrus fruit.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Clove Essential Oil as an Alternative Approach to Control Postharvest Blue Mold Caused by Penicillium italicum in Citrus Fruit

et al. 2019

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“…In the previous studies, postharvest treatment with yeast P. guilliermondii exhibited considerable potential inhibition to the pathogens, such as Colletotrichum gloeosporioides infection in Red Fuji apple, Penicillium digitatum infection in Shogun mandarins, and Botrytis cinerea infection in kiwifruits. [21][22][23] In the present study, P. guilliermondii was found effective in controlling Rhizopus rot and blue mold in peach fruit caused by R. stolonifer and P. expansum, respectively (Figures 1 and 2). The PR1, GLU, and CHI genes in peaches related to defense-related proteins were also differentially expressed following the P. guilliermondii treatment (Figure 8a-c).…”

Section: Discussionmentioning

confidence: 51%

Induced resistance in peach fruit as treated by Pichia guilliermondii and their possible mechanism

Zhao

Zhang

2020

International Journal of Food Properties

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This research aims to assess the effect of Pichia guilliermondii treatment on the improved disease resistance of the peach fruit and to explore the biocontrol mechanisms. Infection rate and lesion diameter of the yeasttreated fruit were substantially inhibited after Rhizopus stolonifer and Penicillium expansum infection, respectively. A significant increase in endogenous salicylic acid (SA) content was stimulated in peach fruit treated with P. guilliermondii. The activities of defense-related enzymes and the SA synthase in peaches were also improved after P. guilliermondii treatment, and the expression of pathogenesis-related genes was activated. Thus, P. guilliermondii may be a biological elicitor-activated systemic acquired resistance by the SA signaling pathway. The new understanding regarding the mechanism of antagonist yeast could could be conducive for controlling fruit postharvest diseases in the future. ARTICLE HISTORY

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“…Quantitative real time (qRT)-PCR ( Exicycler 96, Bioneer, Republic of Korea) was conducted [13, 16] (40 cycles of amplification; denature template at 95 °C for 30 sec, anneal primer at 60 °C for 30 sec, extension at 72 °C for 30 sec) [10, 13] with a KAPA SYBR FAST qPCR kit (Kapa Biosystems, USA) to quantify the transcription abundance [13, 16]. Primers for nine targeted genes were designed as listed in S1 Table as described in previous studies; elongation factor 1 alpha ( EF-1a ) (a housekeeping gene), LOX , CHI , GLU , POD [10], PAL [17], ACS1 , ACO [18] and PR1 [19]. The internal control was performed by the EF-1α transcript [10, 13].…”

Section: Methodsmentioning

confidence: 99%

Targeted transcriptional and proteomic studies explicate specific roles of Bacillus subtilis iturin A, fengycin, and surfactin on elicitation of defensive systems in mandarin fruit during stress

et al. 2019

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Application of Bacillus cyclic lipopeptides (CLPs); fengycin, iturin A and surfactin has shown a great potential in controlling the spread of green mold pathogen invasion ( Penicillium digitatum ) in wounded mandarin fruit during postharvest period. The limited defensive protein profiles followed specific expression of pivotal genes relating to plant hormone mediating signaling pathways of the CLPs’ action on stimulating host plant resistance have been exhibited. The present study aimed to elucidate the specific effect of individual CLP obtained from Bacillus subtilis ABS-S14 as elicitor role on activation of plant defensive system at transcriptional and proteomic levels with and without P . digitatum co-application in mandarin fruit. Fengycin and iturin A elevated the gene expression of PAL , ACS1 , ACO , CHI , and GLU while significantly stimulating plant POD transcription was only detected in the treatments of surfactin both with and without following P . digitatum . An increase of LOX and PR1 gene transcripts was determined in the treatments of individual CLP with fungal pathogen co-application. Fengycin activated production of unique defensive proteins such as protein involved in ubiquinone biosynthetic process in treated flavedo without P . digitatum infection. Proteins involved in the auxin modulating pathway were present in the iturin A and surfactin treatments. CLP-protein binding assay following proteome analysis reveals that iturin A attached to 12-oxophytodienoate reductase 2 involved in the oxylipin biosynthetic process required for jasmonic acid production which is implicated in induced systemic resistance (ISR). This study suggests specific elicitor action of individual CLP, particularly iturin A showed the most powerful in stimulating the ISR system in response to stresses in postharvest mandarins.

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The use of Aloe vera gel coating supplemented with Pichia guilliermondii BCC5389 for enhancement of defense-related gene expression and secondary metabolism in mandarins to prevent postharvest losses from green mold rot

Cited by 28 publications

References 43 publications

Clove Essential Oil as an Alternative Approach to Control Postharvest Blue Mold Caused by Penicillium italicum in Citrus Fruit

Clove Essential Oil as an Alternative Approach to Control Postharvest Blue Mold Caused by Penicillium italicum in Citrus Fruit

Induced resistance in peach fruit as treated by Pichia guilliermondii and their possible mechanism

Targeted transcriptional and proteomic studies explicate specific roles of Bacillus subtilis iturin A, fengycin, and surfactin on elicitation of defensive systems in mandarin fruit during stress

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