The use of amplified fragment length polymorphism (AFLP) in the isolation of sex‐specific markers

Griffiths, Richard; Orr, Kate J.

doi:10.1046/j.1365-294x.1999.00578.x

Cited by 78 publications

(51 citation statements)

References 11 publications

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“…; Mandolino et al 1999: Cannabis sativa; Korpelainen 2002: R. acetosa), mostly by combining bulked segregant analysis (Michelmore et al 1991) with random amplified polymorphic DNA (RAPD; Williams et al 1990). The procedure of the RAPD protocol is less time-consuming than AFLPs (Griffiths and Orr 1999). Moreover, AFLPs are much more costly in terms of laboratory equipment.…”

Section: Introductionmentioning

confidence: 99%

Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes

Stehlik

Blattner

2003

Theor Appl Genet

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We developed SCAR primers based on isolated and sequenced male-specific fragments as identified in an AFLP analysis of the dioecious plant Rumex nivalis. PCR amplification using these primers on females and males resulted in fragments exclusively present in males. Coamplification of the nuclear rDNA internal transcribed spacer 2 together with the male-specific fragment was applied as an internal control for successful PCR reactions to avoid false-negative sex scoring. With a length of about 164 bp, the AFLP fragment was of a similar size as the tandemly arranged, repetitive sequences of 180 bp located on the Y chromosomes of Rumex acetosa. The genetic distances between the Y-chromosomal sequences of R. nivalis and R. acetosa, both members of the section Acetosa, were substantial. We found intra-individual divergence among cloned sequences of the male-specific fragment in R. nivalis. The patterns of interspecific and intra-individual sequence variation found are in accordance with proposed modes of the evolution of sex chromosomes. Y chromosomes possibly arose only once in the genus Rumex and consist mainly of heterochromatic DNA. Due to the almost complete absence of selection on them, Y chromosomes are likely to accumulate large numbers of mutations.

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Section: Introductionmentioning

confidence: 99%

Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes

Stehlik

Blattner

2003

Theor Appl Genet

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“…Amplified Fragment Length Polymorphism (AFLP) method has been used in ostrich (Struthio camels) and shag (Phalacrocorax aristotelis) to sex identification [26,27,28].…”

Section: Aflpmentioning

confidence: 99%

“…In Ratitae, SS and OSM 5 primers were used in sex identification of ostrich which belongs to Ratitae family [36,37]. PCR-based methods to sex-type ostriches were reported [28,36,38]. Identification of sex of the endangered old world vultures by PCR based method, facilitated the breeding of these birds in captivity [39].…”

Section: Chd Genes In Aviansmentioning

confidence: 99%

Identification of Sexes in Poultry Using DNA Based Methods

2017

International Journal of Research Studies in Microbiology and B

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“…Evidence for type and/or presence of sex chromosomes is drawn from physical mapping of sex-specific sequences, from sex-linked linkage maps, from karyological studies and from crosses of sexreversed individuals (Legrand et al, 1987;Sagi and Cohen, 1990;Griffiths and Orr, 1999;Li et al, 2003;Parnes et al, 2003). In Crustacea of the order Decapoda, karyotype studies have revealed distinguishable sex chromosomes only in crabs (Brachyura) and hermit crabs (Anomura), as reviewed by Parnes et al (2003).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

et al. 2011

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In this study, a female-specific DNA marker in the freshwater prawn Macrobrachium rosenbergii was identified through amplified fragment length polymorphism (AFLP). The AFLPderived sequence-characterized amplified region (SCAR) marker was tested in over 200 individuals, giving reproducible sex identification. Further molecular characterization of the sex-marker's genomic region (B3 kb long) revealed the presence of tandem and inverted repeats. The B3-kb sequence was identified both in male and female prawns, but with subtle differences: a deletion of 3 bp (present in female prawn but absent in male prawn) identified upstream of the SCAR marker sequence and two female-specific single-nucleotide polymorphisms, both indicating that male prawns are homozygous, whereas female prawns are heterozygous in this locus. Fluorescent in situ hybridization showed the B3-kb sequence to be unique: to the best of our knowledge, this is the first report of a unique sex-specific sequence observed in situ in crustaceans. The sex-specific marker identified in M. rosenbergii may have considerable applied merit for crustacean culture in that it will enable the determination of genetic sex at early developmental stages when phenotypic differences are not identifiable.

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The use of amplified fragment length polymorphism (AFLP) in the isolation of sex‐specific markers

Cited by 78 publications

References 11 publications

Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes

Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes

Identification of Sexes in Poultry Using DNA Based Methods

Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

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