The use of an IgM immunosorbent agglutination assay to diagnose congenital toxoplasmosis

Skinner, Louise J.; Chatterton, J. M. W.; Joss, A. W. L.; Moir, Isobel L.; Ho-Yen, D. O.

doi:10.1099/00222615-28-2-125

Cited by 29 publications

(6 citation statements)

References 11 publications

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“…The DT and the immunoblot assay were in-house tests and were performed as described previously (13,25). In addition, for determining the infection stage of the seroconverters, an IgM ISAGA (26) and an agglutination test with formalin-fixed tachyzoites (HS agglutination test) (28) were performed essentially as described before. Well-defined sera with known IgG, IgM, IgA, and IgE reactivities to T. gondii were used as internal controls.…”

Section: Methodsmentioning

confidence: 99%

Value of detecting immunoglobulin E antibodies for the serological diagnosis of Toxoplasma gondii infection

Groß

Keksel

Dardé

1997

Clin Diagn Lab Immunol

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The presence of immunoglobulin E (IgE) antibodies was determined by using the immunosorbent-agglutination assay (ISAGA) with 611 serum samples from patients with different clinical conditions to evaluate its value for the serodiagnosis of acute Toxoplasma gondii infection. By analyzing 43 consecutively drawn serum samples from 10 pregnant women who seroconverted, we could show that specific IgE antibodies seem to appear early after infection and are usually present for less than 3 to 5 months. Therefore, we assumed that IgE antibodies seem to be detectable only during the acute or reactivated stage of infection. According to our studies, the IgE ISAGA has an overall sensitivity of only 79.5%, but a specificity of 98.0%, with positive and negative predictive values of 95.5 and 89.8%, respectively. Detection of IgE antibodies in immunosuppressed patients with reactivation of latent T. gondii infection correlates with disease activity. Despite these encouraging results, one must note that IgE antibodies were not detectable in 4 of 14 patients with very recent infection proven by seroconversion. Therefore, detection of IgE antibodies seems to correlate with early acute or reactivated toxoplasmosis, whereas negative IgE results do not exclude the possibility of the acute stage of toxoplasmosis.

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Section: Methodsmentioning

confidence: 99%

Value of detecting immunoglobulin E antibodies for the serological diagnosis of Toxoplasma gondii infection

Groß

Keksel

Dardé

1997

Clin Diagn Lab Immunol

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“…Indeed, ISAgA is known to be highly sensitive and therefore enables the detection of very low levels of specific IgM antibodies in the serum. This allows their detection up to 18 months in congenitally infected infants [5] and even longer after acute infection; to our knowledge up to as long as 3.5 [6] and 5 years [7], respectively. Whatever the case, para sitemia, which is necessary both for triggering the pro duction of specific IgM antibodies and for transmission of the infection to the fetus, occurred long enough before conception not to present a risk for the fetus.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the patient was advised to continue her pregnancy and no treatment was applied. Finally, nega tive ISAgA, which is considered the reference test for congenital toxoplasmosis [4,5] in the newborn baby's blood, in addition to normal clinical appearance, sug gested the baby was not congenitally infected.…”

Section: Discussionmentioning

confidence: 99%

High Levels of IgM Antibodies Specific forToxoplasma gondiiin Pregnancy 12 Years after Primary Toxoplasma Infection

Bobić

Sibalić

Djurković–Djaković

1991

Gynecol Obstet Invest

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A case of high levels of specific IgM antibodies registered by the immunosorbent agglutination assay (ISAgA) in a pregnant woman with a history of toxoplasmosis is reported. The patient had acute lymphoglandular toxoplasmosis diagnosed serologically by increases in the specific antibody titer detected by the Sabin-Feldman test (SFT) and pathohistologically 12 years before pregnancy. In pregnancy, she had stable titers of specific antibodies registered by the SFT, enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT), indirect hemagglutination test (IHAT) and direct agglutination test. Specific IgM antibodies were detected by the ISAgA but not by the IgM-IFAT, IgM-ELISA and IgM-IHAT. She had a normal pregnancy and gave birth to a clinically healthy baby who had a negative ISAgA finding at 7 days of age. This case indicates that ISAgA is not necessarily a marker of recent infection and is therefore not reliable for the diagnosis of toxoplasmosis in pregnancy.

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“…Diferentes técnicas têm a propriedade de detectar esses anticorpos no sangue dos pacientes. Testes como imunofluorescência, hemoaglutinação, immunosorbent agglutination assay (ISAGA), enzyme-linked immunosorbent assay (ELISA) ou microparticle enzyme immunoassay (MEIA) têm papel principal no cenário do diagnóstico da infecção pelo T. gondii (3,11,32,51,63) . Têm sido evidentes certas limitações desses métodos, uma vez que são descritas falhas ao detectar IgG e IgM durante a fase ativa da infecção, e tem sido demonstrado que esses anticorpos podem não ser produzidos durante as primeiras semanas da parasitemia (50) .…”

Section: Sorologiaunclassified

Diagnóstico molecular da toxoplasmose: revisão

Kompalic-Cristo¹,

Britto²,

Fernandes³

2005

J. Bras. Patol. Med. Lab.

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A toxoplasmose infecta milhões de pessoas no mundo inteiro, sendo que a prevalência da infecção humana na maioria dos países está entre 40% e 50%. No Brasil essa taxa aumenta até 80%, dependendo da área estudada. Na maioria dos hospedeiros a infecção é assintomática. A mulher grávida com sorologia negativa pode contribuir para o incremento da morbidade, transmitindo o Toxoplasma gondii para o feto, através da placenta, se adquirir toxoplasmose aguda durante a gravidez. O diagnóstico da toxoplasmose aguda é baseado na detecção de IgM anti-Toxoplasma gondii circulante. A alta sensibilidade das técnicas sorológicas atuais trouxe a realidade da presença de IgM residuais confundindo muitas vezes o diagnóstico final. Nesse sentido, as técnicas moleculares, tais como a reação em cadeia da polimerase (PCR), podem ajudar a uma melhor interpretação do estado real da interação parasito/homem, embora sejam ainda pouco validadas para uso na rotina de diagnóstico laboratorial da toxoplasmose. IntroduçãoO Toxoplasma gondii foi descoberto quase ao mesmo tempo por Nicolle e Manceaux, na Tunísia, e por Splendore, no Brasil, em 1908 (53, 65) . É um protozoário intracelular, que pode parasitar os mais diversos tecidos de vários mamíferos e aves. Seu ciclo de vida é conhecido desde finais da década de 1960, embora muitos pormenores da infecção ainda precisem ser esclarecidos (4) . A toxoplasmose é uma zoonose de distribuição universal que acomete milhões de pessoas no mundo.Em diversos países, têm sido descritas soroprevalências que variam de 15% a 85% na população humana (39) . Em crianças, a soroprevalência é relativamente baixa, aumentando de acordo com a idade, com a exposição a mais fatores de risco durante o transcorrer da vida (1, 11,67) .No Brasil, a soroprevalência tem sido determinada entre

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The use of an IgM immunosorbent agglutination assay to diagnose congenital toxoplasmosis

Cited by 29 publications

References 11 publications

Value of detecting immunoglobulin E antibodies for the serological diagnosis of Toxoplasma gondii infection

Value of detecting immunoglobulin E antibodies for the serological diagnosis of Toxoplasma gondii infection

High Levels of IgM Antibodies Specific forToxoplasma gondiiin Pregnancy 12 Years after Primary Toxoplasma Infection

Diagnóstico molecular da toxoplasmose: revisão

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