The use of BIOCHIP mosaics in diagnostics of bullous pemphigoid: Evaluation and comparison to conventional multistep procedures

Abstract

BackgroundBullous pemphigoid (BP) is an autoimmune blistering disease associated with autoantibodies against BP180 and/or BP230 antigens. The immunoassays available for serological diagnostics include indirect immunofluorescence (IIF) on monkey esophagus (ME), salt‐split skin (SSS), and enzyme‐linked immunosorbent assay (ELISA) for BP180‐NC16a and BP230. Only a few studies validated innovative BIOCHIP mosaic, but none compared agreement between BIOCHIP substrates with conventional methods separately.MethodsWe evaluated the agreement between BIOCHIP and conventional methods and assessed sensitivity and specificity in BP diagnosis. The study comprised 51 BP patients and 39 controls.ResultsAnalysis showed very good agreement between BIOCHIP‐SSS vs classic IIF‐SSS (0.933, P < 0.001) and for BIOCHIP‐BP180‐NC16a vs ELISA‐BP180‐NC16a (0.933, P < 0.001). A good strength of agreement between BIOCHIP‐ME vs classic IIF‐ME was observed (0.694, P < 0.001) similar to BIOCHIP‐BP230 vs ELISA‐BP230 (0.793, P < 0.001). BIOCHIP‐ME sensitivity was 51.0%, whereas IIF‐ME was 76.5%. Epidermal reaction on BIOCHIP‐SSS was found in 94.1% of BP patients and in all patients on IIF‐SSS (sensitivity 100%). BIOCHIP‐BP180‐NC16a sensitivity was lower than in ELISA‐BP180‐NC16a (76.5% vs 82.4%). BP230 sensitivity of both methods was similar (45.1% vs 43.1%). The specificity for all antigens was 100%.ConclusionBIOCHIP mosaic is a useful method presenting satisfactory agreement with conventional immunoassays.

Comparison of the EUROIMMUN Dermatology Profile ELISA to the novel BIOCHIP Mosaic 7 for the diagnosis of immunobullous skin disease

“…[8][9][10][11][12]16,17 This is compared to sensitivities of 45.1-66.7% for the BP230 mosaic on the BIO-CHIP, and a specificity of 39.0-100%. [8][9][10][11][12]16,17 The lower sensitivity of the BP230 mosaic compared to the BP180 mosaic is not unexpected given that it is more common to have autoantibodies against BP180, compared to BP230 in bullous pemphigoid.…”

“…8,9 In bullous pemphigoid, the sensitivity of the BP180 mosaic on the BIOCHIP has been found to range more widely between 55.3 and 100%, [8][9][10][11][12]16,17 with a specificity between 96.5 and 100%. [8][9][10][11][12]16,17 This is compared to sensitivities of 45.1-66.7% for the BP230 mosaic on the BIO-CHIP, and a specificity of 39.0-100%. [8][9][10][11][12]16,17 The lower sensitivity of the BP230 mosaic compared to the BP180 mosaic is not unexpected given that it is more common to have autoantibodies against BP180, compared to BP230 in bullous pemphigoid.…”

Case Report: Pemphigoid Nodularis—Five Patients With Many Years of Follow-Up and Review of the Literature

“…The results were measured by an ELISA plate reader (KHB-ST 360). The standard recommended cutoff values were 20 RU/ml for both BP-180 NC16a and BP-230 ( 9 ).…”

“…In our study, we used the BIOCHIP Dermatology Mosaic 7 (Euroimmun, Lubeck, Germany), which consists of (i) monkey esophagus (BIOCHIP-ME), (ii) salt split skin (BICHIP-SSS), (iii) recombinant BP180 NC16a, (iv) HEK293 cells transfected with BP 230, (v) HEK293 cells transfected with Dsg1, and (vi) HEK293 cells transfected with Dsg3. The assay was carried out in accordance with the manufacturer’s instructions ( 9 ).…”

Low-Power pH Sensor Based on Narrow Channel Open-Gated Al0.25Ga0.75N/GaN HEMT and Package Integrated Polydimethylsiloxane Microchannels

“…It has broad application prospects, namely, clinical diagnosis, industrial control, food and drug analysis (including biopharmaceutical research and development) in the national economy, environmental protection, biotechnology, biochips, and other research areas. With the development of science and technology, biotechnology and electronics infiltrate and merge ulteriorly, therefore more and more electrochemical biosensors have been designed to solve the detection problem of ultra-low concentration [6][7][8].…”