The use of cis-parinaric acid to determine lipid peroxidation in human erythrocyte membranes. Comparison of normal and sickle erythrocyte membranes

Berg, Jeroen J.M. van den; Kuypers, Frans A.; Qju, JH; Chiu, Daniel T.; Lubin, Bertram H.; Roelofsen, B.; Kamp, J.A.F. Op den

doi:10.1016/0005-2736(88)90313-6

Cited by 62 publications

(24 citation statements)

References 25 publications

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“…3) have been used to assess lipid peroxidation. Two often-used lipid-soluble probes are cis-parinaric acid [70][71][72][73][74][75][76][77][78] and C11-BODIPY(581/591) [77,[79][80][81][82][83][84]. cis-Parinaric acid oxidation products are non-fluorescent, thus it is the loss of parent fluorescence intensity that is monitored, whereas C11-BODIPY(581/591) undergoes a green-shift in its fluorescence allowing ratio-based methods to track both the parent and oxidation product(s) [80,85].…”

Section: Do We Know Where Tocopherol Does Its Most Important Job?mentioning

confidence: 99%

The location and behavior of α‐tocopherol in membranes

Atkinson

Harroun

Wassall

et al. 2010

Molecular Nutrition Food Res

173

128

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Vitamin E (a-tocopherol) has long been recognized as the major antioxidant in biological membranes, and yet many structurally related questions persist of how the vitamin functions. For example, the very low levels of a-tocopherol reported for whole cell extracts question how this molecule can successfully protect the comparatively enormous quantities of PUFAcontaining phospholipids found in membranes that are highly susceptible to oxidative attack. The contemporary realization that membranes laterally segregate into regions of distinct lipid composition (domains), we propose, provides the answer. We hypothesize a-tocopherol partitions into domains that are enriched in polyunsaturated phospholipids, amplifying the concentration of the vitamin in the place where it is most needed. These highly disordered domains depleted in cholesterol are analogous, but organizationally antithetical, to the wellstudied lipid rafts. We review here the ideas that led to our hypothesis. Experimental evidence in support of the formation of PUFA-rich domains in model membranes is presented, focusing upon docosahexaenoic acid that is the most unsaturated fatty acid commonly found. Physical methodologies are then described to elucidate the nature of the interaction of a-tocopherol with PUFA and to establish that the vitamin and PUFA-containing phospholipids co-localize in non-raft domains.

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Section: Do We Know Where Tocopherol Does Its Most Important Job?mentioning

confidence: 99%

The location and behavior of α‐tocopherol in membranes

Atkinson

Harroun

Wassall

et al. 2010

Molecular Nutrition Food Res

173

128

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“…The length of the lag phase provides a convenient index for comparison among LDL samples but is frequently misinterpreted as a period during which no changes are occurring in LDL lipids. Rather, the conjugated diene lag phase masks an active period involving radical initiations, radical chain terminations, and antioxidant depletion (13)(14)(15)(16).The oxidative fluorescence decay of parinaric acid (PnA) has been used to monitor lipid peroxidation processes in bilayer systems (18)(19)(20)(21). This polyunsaturated fatty acid is similar in structure to arachidonic acid but contains a conjugated polyene system that accounts for its fluorescent properties and oxidative susceptibility.…”

mentioning

confidence: 99%

Oxidative susceptibility of low density lipoprotein subfractions is related to their ubiquinol-10 and alpha-tocopherol content.

Tribble

Berg

Motchnik

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

135

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The conjugated polyene fatty acid parinaric acid (PnA) undergoes a stoichiometric loss in fluorescence upon oxidation and can be used to directly monitor peroxidative stress within lipid environments. We evaluated the course of potentially atherogenic oxidative changes in low density lipoproteins (LDL) by monitoring the oxidation of PnA following its incorporation into buoyant (p = 1.026-1.032 g/ml) and dense (p = 1.040-1.054 g/ml) LDL subfractions. Copperinduced oxidation of LDL-associated PnA exhibited an initial lag phase followed by an increased rate of loss until depletion. Increased PnA oxidation occurred immediately after the antioxidants ubiquinol-10 and a-tocopherol were consumed but before there were marked elevations in conjugated dienes. Despite differences in sensitivity to early oxidation events, PnA oxidation and conjugated diene lag times were correlated (r = 0.582; P = 0.03), and both indicated a greater susceptibility of dense than buoyant LDL in accordance with previous reports. The greater susceptibility of PnA in dense LDL was attributed to reduced levels of ubiquinol-10 and a-tocopherol, which were =50% lower than in buoyant LDL (mol of antioxidant/mol of LDL) and together accounted for 80% of the variation in PnA oxidation lag times. These results suggest that PnA is a useful probe of LDL oxidative susceptibility and may be superior to conjugated dienes for monitoring the initial stages of LDL lipid peroxidation. Differences in oxidative susceptibility among LDL density subfractions are detected by the PnA assay and are due in large part to differences in their antioxidant content.Free-radical-mediated oxidation induces potentially atherogenic changes in low density lipoproteins (LDL) (1). Chief among these is an ability to stimulate cholesterol engorgement by macrophages (2, 3), which are progenitors of lipidladen foam cells found in early atherosclerotic lesions. In addition, oxidized-LDL influences the chemotactic behavior of monocytes and monocyte-derived macrophages (4, 5), promotes endothelial cell damage (6, 7), and induces potent mediators of cell proliferation, monocyte adherence, and platelet aggregation (8-12), all of which may contribute to lesion formation and growth.LDL oxidation is believed to proceed through initial oxidation of the fatty acyl components. The resulting lipidderived radicals are intercepted by radical scavenging antioxidants including ubiquinol-10 and a-tocopherol. However, if the oxidant stress is sufficiently great or prolonged, these antioxidants are depleted (13)(14)(15)(16) and injury is propagated among LDL lipids with spread to the protein component through reactive lipid breakdown products (6,17). The atherogenic properties of oxidized-LDL are attributed to this extensively altered particle (1)(2)(3)(4)(5)(6)(7)17), although minimal modifications associated with the initial stages of injury also may be important (10-12).As evidence has accumulated in support of a role for LDL oxidation in atherogenesis, assays of oxidative susceptibility hav...

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“…Регистрация флуоресценции цис-паринариевой кислоты в присутствии липидов, экстрагированных из эритроцитов крови крыс. Для регистрации флуоресценции цис-паринариевой кислоты в присутствии липидов, экстрагированных из эритроцитов крови крыс, использовали стандартную методику [12][13][14][15]20] …”

Section: методикаunclassified

“…Для этого мы использовали метод, основанный на измерении флуоресценции цис-паринариевой кислоты (цисПК) [12][13][14][15]20]. Известно, что флуоресценция цисПК снижается при её окислении свободными радикалами.…”

Section: при обработке результатов рассчитывали процент падения флуорunclassified

Effects of low power laser radiation of blue, green and red ranges on free radical processes in rat blood in endotoxic shock

IuA

et al. 2013

BIOMED KHIM

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Целью данной работы было исследование влияния низкоинтенсивного лазерного излучения синего (441,2 нм), зелёного (532,5 нм) и красного (632,8 нм) диапазонов на свободнорадикальные процессы в крови крыс при экспериментальном эндотоксическом шоке. В работе использовали модель эндотоксического шока, получаемую интраперитонеальным введением липополисахарида В (25 мг/кг). Были проведены измерения функциональной активности лейкоцитов крови (методом люминол-зависимой хемилюминесценции), супероксиддисмутазной активности плазмы крови (методом с нитросиним тетразолием) и окисления липидов мембран эритроцитов (по измерению флуоресценции цис-паринариевой кислоты). Обнаружено, что низкоинтенсивное лазерное излучение оказывает сильное влияние на все исследуемые процессы как в группах животных, которым вводили липополисахарид В, так и в контрольных группах. Наиболее выраженное действие излучения наблюдали в группах животных, которым вводили липополисахарид. В этих группах лазерное излучение вызывало возрастание всех исследуемых показателей. При дозе облучения (0,75 Дж/см 2 ) наиболее эффективным оказалось излучение в зеленом диапазоне спектра. При дозе 1,5 Дж/см 2 сильное влияние оказывало излучение, как в зелёной, так и в красной областях спектра. Обсуждаются возможные механизмы наблюдаемых процессов.Ключевые слова: эндотоксический шок, низкоинтенсивное лазерное излучение, хемилюминесценция, активные формы кислорода, лейкоциты, супероксиддисмутазная активность, свободнорадикальное окисление липидов.ВВЕДЕНИЕ. Сепсис (эндотоксический шок, синдром системного воспаления (ССВ)) и последующий синдром полиорганной недостаточности (СПН) являются наиболее серьезными проявлениями внедрения бактериальной инфекции. Сегодня СПН -одна из частых причин смерти и довольно распространенный патофизиологический процесс. Около 1% всех больных и до 20-30% больных в реанимационных палатах так или иначе имеют 411 * -адресат для переписки

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The use of cis-parinaric acid to determine lipid peroxidation in human erythrocyte membranes. Comparison of normal and sickle erythrocyte membranes

Cited by 62 publications

References 25 publications

The location and behavior of α‐tocopherol in membranes

The location and behavior of α‐tocopherol in membranes

Oxidative susceptibility of low density lipoprotein subfractions is related to their ubiquinol-10 and alpha-tocopherol content.

Effects of low power laser radiation of blue, green and red ranges on free radical processes in rat blood in endotoxic shock

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