The Use of Culture Filtrate of the Fungus to Screen for Verticillium Albo-Atrum in Strawberry.

Gaggioli, D.; Maas, John L.; Mezzetti, Bruno; Rosati, P.

doi:10.17660/actahortic.1989.265.5

Cited by 4 publications

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“…Sowik et al (2001) suggested that in vitro screening of strawberry could be useful for identifying somaclonal variants tolerant to infection with Verticillium dahliae, since they observed that the reaction of strawberry cultivars in vitro was similar to their sensitivity to Verticillium wilt in the field. However, this is in contrast to Gaggioli et al (1989) who were unsuccessful in developing an in vitro assay to screen for resistance to V. dahliae.…”

Section: Inoculation Evaluations For Strawberry Cultivarsmentioning

confidence: 87%

In vitro response of strawberry cultivars and regenerants to Colletotrichum acutatum

Hammerschlag¹,

Garcés²,

Koch-Dean³

et al. 2006

Plant Cell Tiss Organ Cult

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Diseases affecting strawberry (Fragaria Â ananassa Duch.) have been of major concern in recent years because of their widespread occurrence and potential for yield loss. Anthracnose, caused by the fungus Colletotrichum acutatum, is one of the most serious diseases of strawberry worldwide. Tissue-culture induced (somaclonal) variation provides one strategy for generating disease-resistant genotypes. As part of a program to generate strawberry germplasm resistant to anthracnose, an in vitro screening system was used to evaluate several commercial cultivars, Chandler, Delmarvel, Honeoye, Latestar, Pelican and Sweet Charlie propagated in vitro, and shoots regenerated from leaf explants of these cultivars for resistance to C. acutatum isolate Goff (highly virulent). Regenerants with increased levels of resistance were identified from all of the cultivars. The greatest increases in disease resistance were observed for regenerants from leaf explants of cultivars Pelican and Chandler that exhibited 17.5-and 6.2-fold increases in resistance, respectively. The highest levels of anthracnose resistance (2 to 6% leaf necrosis) were exhibited by regenerants from explants of cultivars Pelican and Sweet Charlie. These studies suggest that generating somaclonal variation may be a viable approach to obtaining strawberry plants with increased levels of anthracnose resistance.

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Section: Inoculation Evaluations For Strawberry Cultivarsmentioning

confidence: 87%